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pMRE-Himar-131

Plasmid

#185923

Purpose

Himar transposon delivery plasmid that delivers a cyan fluorescent mTurquoise2 protein gene

Depositor

Mitja Remus-Emsermann

Article

Stocks et al Bacteria 2022, 1(1), 56-65

Insert

mTurquoise2

Expression

Bacterial

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pNJB80

Plasmid

#51518

Purpose

Gateway entry vector with attL5 and attL2 sites flanking a CmR and ccdB gene. In our hands, the multisite reaction still proceeds efficiently.

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Type

Empty backbone

Use

Gateway entry vector

Available Since

March 6, 2014

Availability

Academic Institutions and Nonprofits only

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pGCS-C2m(3xHA)

Plasmid

#85725

Purpose

Gateway destination vector

Depositor

Hai-Ning Du

Article

Wang et al Acta Biochim Biophys Sin (Shanghai). 201

Type

Empty backbone

Use

Mammalian/avian/xenopus/zebrafish

Tags

3xHA

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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LSL

Plasmid

#66590

Purpose

Lox-STOP-Lox reporter

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

LSL-Tomato

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pAO139 (Apr LEU2 mrcl-AQ)

Plasmid

#41924

Depositor

Stephen Elledge

Article

Osborn et al Genes Dev. 2003 Jul 15;17(14):1755-67.

Insert

Mediator of the Replication Checkpoint

Mutation

MRC1AQ

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
pGGF000

Plasmid

#48861

Purpose

Empty entry vector for creating GreenGate plant resistance cassette modules.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pBbS5c-Maqu_3494

Plasmid

#86205

Purpose

Expresses efflux pump in E. coli to provide resistance to biofuel (low copy)

Depositor

Mary Dunlop

Article

Turner et al ACS Synth Biol. 2015 Oct 16;4(10):1056-6

Insert

Efflux pump

Expression

Bacterial

Promoter

ATCGTTTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAA…

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pAG306-GPD-ccdB chr I

Plasmid

#41894

Depositor

Dan Gottschling

Article

Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

Type

Empty backbone

Expression

Yeast

Promoter

GPD

Available Since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pJJH1320

Plasmid

#36915

Depositor

Jürgen Heinisch

Article

Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

Insert

Cre recombinase

Expression

Bacterial

Available Since

July 17, 2012

Availability

Academic Institutions and Nonprofits only

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pDO23A

Plasmid

#27367

Depositor

Dirk Schnappinger

Article

Blumenthal et al PLoS One. 2010 Dec 22;5(12):e15667. doi:

Insert

pUC/ori, bla

Use

Gateway donor vector

Expression

Bacterial

Mutation

N/A

Available Since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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pJBL2801

Plasmid

#71207

Purpose

AD1 Sense Expression Plasmid

Depositor

Julius Lucks

Article

Chappell et al Nat Chem Biol. 2015 Mar;11(3):214-20. do

Insert

AD1 Sense

Use

Synthetic Biology

Expression

Bacterial

Promoter

BBa_J23119

Available Since

Jan. 21, 2017

Availability

Academic Institutions and Nonprofits only

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lvl0 alcSynth-34S

Plasmid

#153411

Purpose

Ethanol system inducible promoter

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

alcSynth-34S promoter

Expression

Plant

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pMU102

Plasmid

#61805

Purpose

Cloning and expression vector for Clostridium thermocellum

Depositor

Lee Lynd

Article

Olson et al J Biol Eng. 2012 May 11;6(1):5. doi: 10.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pINS-Blast

Plasmid

#31388

Depositor

Yegor Vassetzky

Article

Dmitriev et al BMC Res Notes. 2008 Dec 23;1:135. doi: 1

Insert

bsd

Use

Cre/Lox

Available Since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only

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MSCV-TRE-DsRed-IRES-d24EGFP

Plasmid

#41945

Purpose

Retroviral expression of DsRed in mammalian cells

Depositor

Stephen Elledge

Article

Yen et al Science. 2008 Nov 7;322(5903):918-23. do

Insert

DsRed

Use

Retroviral

Expression

Mammalian

Promoter

TRE

Available Since

May 2, 2013

Availability

Academic Institutions and Nonprofits only

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pSM040

Plasmid

#128294

Purpose

asymmetric plasmid partitioning E. coli - target plasmid

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

Piq - mRFP, parS

Expression

Bacterial

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pMYT018

Plasmid

#180671

Purpose

Encodes rtTA as a Type 3 part to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

rtTA

Use

Synthetic Biology

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT015

Plasmid

#180668

Purpose

Encodes pTet7 as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

pTet7

Use

Synthetic Biology

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAO122 (Apr LEU2 MRC1)

Plasmid

#41923

Depositor

Stephen Elledge

Article

Osborn et al Genes Dev. 2003 Jul 15;17(14):1755-67.

Insert

Mediator of the Replication Checkpoint

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
p{ie1-DsRed::GW,attB}

Plasmid

#182173

Purpose

fluorescent reporter gene with intron containing Gateway cloning cassette

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Expression

Insect

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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p{ie1-eGFP::GW,attB}

Plasmid

#182172

Purpose

fluorescent reporter gene with intron containing Gateway cloning cassette

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

eGFP

Expression

Insect

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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p7XC3H

Plasmid

#47065

Purpose

FX cloning E.coli expression vector with T7 promoter and C-terminal 3C protease cleavage site and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Bacterial

Promoter

T7

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pACYC.GFP1Amb

Plasmid

#102792

Purpose

Expresses GFP with a premature stop codon

Depositor

Andrew Ellington

Article

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Insert

Green Fluorescent Protein

Expression

Bacterial

Mutation

Y39Stop

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAC-EsaR-EsaI

Plasmid

#47660

Purpose

esaI gene downstream of esaR in pAC-EsaR (CmR)

Depositor

Cynthia Collins

Article

Shong et al ACS Synth Biol. 2013 Jul 23.

Insert

esaI-term

Use

Synthetic Biology

Expression

Bacterial

Promoter

Plac

Available Since

Sept. 6, 2013

Availability

Academic Institutions and Nonprofits only
pdestMB14

Plasmid

#26415

Depositor

Marc Vidal

Article

Dupuy et al Genome Res. 2004 Oct;14(10B):2169-75.

Insert

attR4-ccdB-attR2

Expression

Bacterial and Worm

Available Since

April 23, 2012

Availability

Academic Institutions and Nonprofits only

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pGGB000

Plasmid

#48857

Purpose

Empty entry vector for creating GreenGate N-terminal tag modules.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pXDC61-RalF

Plasmid

#21844

Depositor

Howard Shuman

Article

Charpentier et al PLoS Pathog. 2009 Jul . 5(7):e1000501.

Insert

RalF

Tags

TEM

Expression

Bacterial

Available Since

Sept. 2, 2009

Availability

Academic Institutions and Nonprofits only

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pdestDD03

Plasmid

#26414

Depositor

Marc Vidal

Article

Dupuy et al Genome Res. 2004 Oct;14(10B):2169-75.

Insert

attR4-ccdB-attR2 cassette

Expression

Bacterial and Worm

Available Since

April 23, 2012

Availability

Academic Institutions and Nonprofits only

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pdCas9-AsiA

Plasmid

#158063

Purpose

Expressing CasTA of dCas9 fused AsiA

Depositor

Harris Wang

Article

Ho et al Mol Syst Biol. 2020 Jul;16(7):e9427. doi

Insert

dCas9-AsiA

Expression

Bacterial

Promoter

TetO

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pBXC3H

Plasmid

#47068

Purpose

FX cloning E.coli expression vector with araBAD promoter and C-terminal 3C protease cleavage site and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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p7XC3GH

Plasmid

#47066

Purpose

FX cloning E.coli expression vector with T7 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Bacterial

Promoter

T7

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pCVD433

Plasmid

#63756

Purpose

Derivative of pACYC184 M1uI linkers inserted into EcoRV site inactivating Tc resistance gene. Now Tcs

Depositor

James Kaper

Article

Donnenberg et al Infect Immun. 1990 Jun;58(6):1565-71.

Type

Empty backbone

Expression

Bacterial

Available Since

April 6, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX6P1-DEST-HA

Plasmid

#119751

Purpose

Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal HA tag.

Depositor

Andrew Jackson, Martin Reijns

Type

Empty backbone

Tags

GST and HA

Expression

Bacterial

Promoter

tac promoter

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMA_Level 2A

Plasmid

#102705

Purpose

Mobius Assembly Level 2 Acceptor Vector A

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

J23106:B0034:sfGFP:rrnBT1-T7TE

Use

Synthetic Biology

Available Since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGM000

Plasmid

#48864

Purpose

Empty entry vector for creating GreenGate supermodules w/o plant resistance cassette.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGN000

Plasmid

#48865

Purpose

Empty entry vector for creating GreenGate supermodules with plant resistance cassette.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pPSSUB-101

Plasmid

#26580

Depositor

Ron Weiss

Article

Basu et al Proc Natl Acad Sci U S A. 2004 Apr 27. 1

Insert

destabilized GFP

Expression

Bacterial

Mutation

S2R, C-terminal extension RPAANDENYA(LVA)

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pAA6

Plasmid

#128282

Purpose

asymmetric plasmid partitioning E. coli - target plasmid

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

Piq- mRFP, parS

Expression

Bacterial

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pXDC61-FabI

Plasmid

#21842

Depositor

Howard Shuman

Article

Charpentier et al PLoS Pathog. 2009 Jul . 5(7):e1000501.

Insert

fabI

Tags

TEM

Expression

Bacterial

Available Since

Sept. 2, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGE000

Plasmid

#48860

Purpose

Empty entry vector for creating GreenGate terminator modules.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGCS-C1m(5xMYC)

Plasmid

#85724

Purpose

Gateway destination vector

Depositor

Hai-Ning Du

Article

Wang et al Acta Biochim Biophys Sin (Shanghai). 201

Type

Empty backbone

Use

Mammalian/avian/xenopus/zebrafish

Tags

5xMYC

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBLO 9.2_bCas9-CP199

Plasmid

#121496

Purpose

TetR_tetR/tetA promoters_dCas9-CP_CM_P15A

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Type

Empty backbone

Expression

Bacterial

Promoter

p15A

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
EYK054

Plasmid

#69512

Purpose

pBAD pduP1-18-gfpmut2-ssrA

Depositor

Danielle Tullman-Ercek

Article

Jakobson et al J Biol Chem. 2015 Aug 17. pii: jbc.M115.

Insert

GFPmut2

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_WTDHFR_WTTS

Plasmid

#191282

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Expression

Bacterial

Mutation

WT DHFR, WT TYMS

Promoter

T7 (folA), Tet (thyA)

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pVS45

Plasmid

#20059

Depositor

Stephen Sligar

Article

Beck von Bodman et al Proc Natl Acad Sci U S A. 1986 Dec . 83(

Insert

rat hepatic cytochrome b5

Expression

Bacterial

Available Since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only
pMRE-Himar-175

Plasmid

#185943

Purpose

Himar transposon delivery plasmid that delivers a red fluorescent mScarlet-I protein gene

Depositor

Mitja Remus-Emsermann

Article

Stocks et al Bacteria 2022, 1(1), 56-65

Insert

mScarlet-I

Expression

Bacterial

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
lvl0 mTFP1 CTAG

Plasmid

#153400

Purpose

Cyan fluorescent protein with FLAG tag

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

mTFP1 CTAG

Tags

FLAG tag

Expression

Plant

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pKP1141

Plasmid

#160811

Purpose

Expression of TonB protein

Depositor

Kathleen Postle

Article

Gresock et al Front Microbiol. 2011 Oct 12;2:206. doi:

Insert

tonB (tonB Escherichia coli)

Expression

Bacterial

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC-PHYT

Plasmid

#19002

Depositor

Jay Keasling

Article

Smolke et al Metab Eng. 2001 Oct . 3(4):313-21.

Insert

crtE, crtB

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
lvl0 G10-90p

Plasmid

#153370

Purpose

Synthetic promoter for plant expression

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

G10-90 promoter

Expression

Plant

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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