Addgene

We narrowed to 9,789 results for: otos

Showing: 3481 - 3510 of 9789 results

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pET17_K560_GFP_His

Plasmid

#15219

Depositor

Ron Vale

Article

Woehlke et al Cell. 1997 Jul 25. 90(2):207-16.

Insert

Human neuronal kinesin heavy chain (KIF5B Human)

Use

Tags

GFP and His

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pRDV5 containing Sb-loop

Plasmid

#132695

Purpose

pRDV5 plasmid containing a loop Sybody for the generation of a qPCR standard curve

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

Sb_loop including 5' and 3' flanking sequences used for ribosome display

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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NLS-SuperNova

Plasmid

#131858

Purpose

Expresses SuperNova fused to nuclear localization signal (NLS)

Depositor

Geert van den Bogaart

Article

Paardekooper et al Int J Mol Sci. 2019 Aug 25;20(17). pii:

Insert

SuperNova

Use

Tags

Myc tag and c-myc nuclear localization signal (NL…

Expression

Mammalian

Mutation

Promoter

cmv

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-tdTomato-P2A-paCaMKII

Plasmid

#165431

Purpose

Expresses tdTomato and paCaMKII in mammalian cells to induce synaptic plasticity.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

tdTomato-P2A-paCaMKII

Use

Tags

tdTomato

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_r5-HTmut

Plasmid

#208720

Purpose

Expresses the red 5-HT sensor GRAB_r5-HTmut in neurons

Depositor

Yulong Li

Article

Deng et al bioRxiv 2023.05.27.542566

Insert

Red fluorescent 5-HT sensor GRAB_r5-HTmut

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-Brainbow3.2-PGK-PuroTK

Plasmid

#159928

Purpose

Brainbow transgenesis

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Stem Cell Res. 2021 Jan 8;51:102164. doi

Inserts

NFPnls
mOrange2
EGFP
mKate2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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flex-ChrimsonR-EYFP-Kv

Plasmid

#135319

Purpose

Cre-dependent expression of soma-targeted ChrimsonR

Depositor

Hongkui Zeng

Article

Hage et al Elife. 2022 Jan 21;11:e71103. doi: 10.75

Insert

ChrimsonR

Use

AAV

Tags

EYFP

Expression

Mutation

Promoter

synapsin

Available since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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TagRFP-T-VAPB(1- 218)- eMagB

Plasmid

#162255

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

TagRFP-T-VAPB(1- 218)- eMagB

Use

Tags

Expression

Mammalian

Mutation

Promoter

beta-actin promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-FIP3

Plasmid

#74753

Purpose

Expressed sfGFP-tagged FIP3 in E. coli for protein production

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

Rab11 family interacting protein 3 (RAB11FIP3 Human)

Use

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 13, 2016

Availability

Academic Institutions and Nonprofits only

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AzoRS-4

Plasmid

#182884

Purpose

A pEvol-based plasmid containing one inducible (by arabinose) copy of the AzoRS-4 aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.

Depositor

Miriam Amiram

Article

Israeli et al Adv. Funct. Mater. 2021, 31, 2011276

Insert

AzoRS-4

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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ClCry4 PHR

Plasmid

#160552

Purpose

Expression of protein for various experiments including finding interaction partners

Depositor

Joseph Takahashi

Article

Zoltowski et al Proc Natl Acad Sci U S A. 2019 Sep 24;11

Insert

Clcry4 HR

Use

Tags

Hexa his

Expression

Insect

Mutation

Promoter

Available since

Nov. 6, 2020

Availability

Academic Institutions and Nonprofits only

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FusionRed-Lifeact-7

Plasmid

#54778

Purpose

Localization: Actin, Excitation: 580, Emission: 608

Depositor

Michael Davidson

Article

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Type

Empty backbone

Use

Tags

FusionRed-Lifeact

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
Lenti_sgRNA_MS2_neo

Plasmid

#118650

Purpose

To clone sgRNA for activation dCas9

Depositor

Christopher Vakoc

Article

Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pRS415_pGal-nCas9(D10A)

Plasmid

#79618

Purpose

Expresses nCas9(D10A) in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Use

Tags

Expression

Yeast

Mutation

D10A for nickase Cas9

Promoter

pGal1

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pHUC-JEDI-1P

Plasmid

#202619

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-1P under the HuC promoter for pan neuronal expression in zebrafish; JEDI-1P sequence codon-optimized for zebrafish expression

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

JEDI-1P

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

HuC

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pGP-CAG-iGABASnFR2n-WPRE-bGH-polyA

Plasmid

#218869

Purpose

Mammalian expression of improved GABA sensor (negative change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al bioRxiv 2025.03.25.644953

Insert

iGABASnFR2n

Use

Tags

Expression

Mammalian

Mutation

S99A F104H R168P

Promoter

CAG

Available since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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CAG-DIO-mRuby2

Plasmid

#104058

Purpose

AAV expression of Cre-dependent reporter mRuby2

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

mRuby2

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCCl-MCS_miniCMV_EGFP

Plasmid

#134984

Purpose

The plasmid includes a EGFP gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

Depositor

Claudio Mussolino

Article

Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

minCMV

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-3xFLAG-NCOA4 (dIDR2)

Plasmid

#192811

Purpose

Stable expression of 3xFLAH-NCOA4 (delta IDR2) in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

NCOA4 (NCOA4 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Deletion of the IDR2

Promoter

Available since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCSC-NES-RFP

Plasmid

#221812

Purpose

Lentiviral vector expressing red fluorescent protein (RFP) fused with nuclear export sequence (NES)

Depositor

Baojin Ding

Article

Akter et al J Neurosci. 2024 Apr 10;44(15):e17282320

Insert

NES-mCherry

Use

Lentiviral

Tags

NES

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-3xFLAG-NCOA4 (dN)

Plasmid

#192808

Purpose

Stable expression of 3xFLAH-NCOA4 (delta N) in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

NCOA4 (NCOA4 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Deletion of the N-terminal domain

Promoter

Available since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-3xFLAG-NCOA4 (dM)

Plasmid

#192810

Purpose

Stable expression of 3xFLAH-NCOA4 (delta M) in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

NCOA4 (NCOA4 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Deletion of the Middle domain

Promoter

Available since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-3xFLAG-NCOA4 (dIDR1)

Plasmid

#192809

Purpose

Stable expression of 3xFLAH-NCOA4 (delta IDR1) in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

NCOA4 (NCOA4 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Deletion of the IDR1

Promoter

Available since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-3xFLAG-NCOA4 (dC)

Plasmid

#192812

Purpose

Stable expression of 3xFLAH-NCOA4 (delta C) in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

NCOA4 (NCOA4 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Deletion of the C-terminal domain

Promoter

Available since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-3xFLAG-NCOA4 (I56E)

Plasmid

#192813

Purpose

Stable expression of 3xFLAH-NCOA4 (I56E mutant) in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

NCOA4 (NCOA4 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

I56E mutation in the N-terminal domain

Promoter

Available since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-3xFLAG-NCOA4 (L63R)

Plasmid

#192814

Purpose

Stable expression of 3xFLAH-NCOA4 (L63R mutant) in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

NCOA4 (NCOA4 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

L63R mutation in the N-terminal domain

Promoter

Available since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-OTp-mCherry

Plasmid

#184754

Purpose

To drive mCherry under the control of mouse oxytocin promoter

Depositor

Kazunari Miyamichi

Article

Inada et al Neuron. 2022 Apr 12. pii: S0896-6273(22)

Insert

mCherry

Use

AAV

Tags

Expression

Mutation

Promoter

mouse Oxytocin promoter

Available since

May 27, 2022

Availability

Academic Institutions and Nonprofits only

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pMax-E2F1

Plasmid

#16007

Depositor

Olimpia Meucci

Article

Shimizu et al Neurobiol Dis. 2007 Jan . 25(1):17-26.

Insert

E2F1 (E2F1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2007

Availability

Academic Institutions and Nonprofits only

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pTE5400_pMBP_CDS dropout vector

Plasmid

#221189

Purpose

Expression vector for N-terminally His-tagged maltose binding protein (10xHis-MBP) fused to a protein of interest

Depositor

Tobias Erb

Article

Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

Type

Empty backbone

Use

Tags

10x His-tag + E. coli maltose binding protein (MB…

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-Mito-mRFP

Plasmid

#169001

Purpose

Express Mito (Su9)-mRFP in zebrafish

Depositor

Noboru Mizushima

Article

Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

Insert

Mito (Su9)-mRFP (encoding a mitochondrial matrix targeting sequence of subunit 9 of the V0 ATPase Su9 of N. crassa)

Use

Unspecified

Tags

mRFP

Expression

Mutation

Promoter

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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