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We narrowed to 26,115 results for: Nes;

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Showing: 4681 - 4720 of 26115 results

  4. Antares-SEPLuc C1A4E

    Plasmid
    #141088
    Purpose
    precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES and weaker precursor of Nanoluc
    Depositor
    Jeak Ling Ding
    Article
    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41
    Insert
    Antares-T2A-puromycin-IRESv24-SEP-C1A4E
    Expression
    Mammalian
    Mutation
    Please see depositor comments
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    141088-plasmid-map-sequence-id-296867

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  6. pCIG1_UL136_19kDa_EE

    Plasmid
    #74951
    Purpose
    Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 19kda isoform of UL136. Also expresses EGFP.
    Depositor
    Felicia Goodrum
    Article
    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:
    Insert
    UL136 (UL136 Human Cytomegalovirus strain TB40/E)
    Use
    Lentiviral
    Tags
    Glu-Glu tag (EYMPME)
    Expression
    Mammalian
    Mutation
    delta 1-127
    Promoter
    CMV, lentiviral LTR
    Available Since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    74951-plasmid-map-sequence-id-218061

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  7. pCIG1_UL136_23kDa_Myc

    Plasmid
    #74949
    Purpose
    Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 23kda isoform of UL136. Also expresses EGFP.
    Depositor
    Felicia Goodrum
    Article
    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:
    Insert
    UL136 (UL136 Human Cytomegalovirus strain TB40/E)
    Use
    Lentiviral
    Tags
    HA Tag (YPYDVPDYA)
    Expression
    Mammalian
    Mutation
    delta 1-99 M128A
    Promoter
    CMV, Lentiviral LTR
    Available Since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    74949-plasmid-map-sequence-id-218062

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  8. CoxVIIIx2-CeNL(Ca2+)_1.2μ-pcDNA3

    Plasmid
    #111922
    Purpose
    Cyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_1.2μ
    Depositor
    Takeharu Nagai
    Article
    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/
    Insert
    CeNL(Ca2+)_1.2μ
    Tags
    CoxVIIIx2
    Expression
    Mammalian
    Mutation
    E67D, E104D, D133E at CaM
    Promoter
    CMV
    Available Since
    June 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    111922-plasmid-map-sequence-id-214013

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  11. PatoL-LOG241

    Plasmid
    #72846
    Purpose
    Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). LOG241 backbone.
    Depositor
    Chris Barnes
    Article
    Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278
    Insert
    PatoL
    Expression
    Bacterial
    Promoter
    PatoL
    Available Since
    Aug. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    72846-plasmid-map-sequence-id-133612

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  26. pCIG1_UL136_25kDa_Myc

    Plasmid
    #74954
    Purpose
    Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 25kda isoform of UL136. Also expresses EGFP.
    Depositor
    Felicia Goodrum
    Article
    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:
    Insert
    UL136 (UL136 Human Cytomegalovirus strain TB40/E)
    Use
    Lentiviral
    Tags
    3xFLAG (GGGGDYKDDDDKGADYKDDDDKEFDYKDDDDK)
    Expression
    Mammalian
    Mutation
    delta 1-77, M100A M128A
    Promoter
    CMV, Lentiviral LTR
    Available Since
    May 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    74954-plasmid-map-sequence-id-141774

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  30. ML43

    Bacterial Strain
    #61922
    Purpose
    C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. cyo ilvE avtA aspC hisG argH metA lysA thrC asnA asnB genes deleted.
    Depositor
    Robert Gennis, Toshio Iwasaki
    Article
    Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1
    Bacterial Resistance
    None
    Available Since
    Jan. 26, 2015
    Availability
    Academic Institutions and Nonprofits only

  31. ML42

    Bacterial Strain
    #61921
    Purpose
    C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. cyo ilvE avtA aspC hisG argH metA lysA thrC asnB genes deleted.
    Depositor
    Robert Gennis, Toshio Iwasaki
    Article
    Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1
    Bacterial Resistance
    None
    Available Since
    Jan. 26, 2015
    Availability
    Academic Institutions and Nonprofits only

  32. Transposon-for RMCE_Sleeping Beauty_LIR-CAG-Frt-red(katushka)-P2A-Puro-F3-PA-RIR

    Plasmid
    #67275
    Purpose
    For sleeping beauty transposition and establishment of an recombinase mediated cassette exchange docking site. To make RMCE in cell line instead of flp in cell lines
    Depositor
    Jannik Elverløv-Jakobsen
    Article
    Callesen et al PLoS One. 2016 Aug 19;11(8):e0161471. do
    Insert
    puromycin resistant gene
    Tags
    linked to red flouresent protein gene (katushka)
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    July 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    67275-plasmid-map-sequence-id-118496

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  37. VP12

    Plasmid
    #72247
    Purpose
    Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A and Q926A
    Promoter
    CMV
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72247-plasmid-map-sequence-id-485302

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  38. YopH (residues 164-468)

    Plasmid
    #79749
    Purpose
    This plasmid encodes the active catalytic domain (amino acid residues 164-468) of YopH. Inteded for co-expression with human Tyr kinase plasmids to enhance bacterial kinase expression.
    Depositor
    John Chodera, Nicholas Levinson, Markus Seeliger
    Article
    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/
    Insert
    YopH (residues 164-468)
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    79749-plasmid-map-sequence-id-443904

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Showing: 4681 - 4720 of 26115 results