Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 626 results for: SPEN

Showing: 551 - 600 of 626 results

  3. pGG442

    Plasmid
    #165616
    Purpose
    Vector for expression of SpCas9-Zif268 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t (KanR cassette in place of the PID)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    TEF1p-SpCas9::KanR(in place of PID)-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t
    Use
    CRISPR
    Tags
    3x HA, SV40 NLS, Zif268, and c-Myc-like NLS
    Expression
    Yeast
    Mutation
    Corrected homology relative to wild type SpCas9 (…
    Promoter
    TEF1
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165616-plasmid-map-sequence-id-330958

    Enlarge

  5. pRM14

    Plasmid
    #163693
    Purpose
    hsp70 promoter inducible expression of DHB:mNeonGreen-P2A-H2B:mScarlet
    Depositor
    David Matus
    Article
    Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1
    Insert
    DHB:mNeonGreen-P2A-H2B:mScarlet (HELB, H2BC11 Human)
    Use
    Unspecified; Zebrafish expression
    Tags
    mNeonGreen and mScarlet
    Expression
    Mutation
    Promoter
    HSP70
    Available since
    March 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    163693-plasmid-map-sequence-id-324024

    Enlarge

  7. pGL4.22-PGK1-HRE::dLUC

    Plasmid
    #128095
    Purpose
    Hypoxia response element real-time luciferase reporter
    Depositor
    Chi Van Dang
    Article
    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:
    Insert
    Hypoxia response element (x3) from PGK1 promoter (PGK1 Human)
    Use
    Luciferase
    Tags
    drives expression of destabilized luciferase (2CP…
    Expression
    Mammalian
    Mutation
    Promoter
    inserted PGK1 hypoxia response elements (HRE) (x3)
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    128095-plasmid-map-sequence-id-249667

    Enlarge

  9. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP

    Plasmid
    #112677
    Purpose
    An AAV vector that expresses a Cre-dependent nuclear-localized Red to Green Fluorescent protein
    Depositor
    Brandon Harvey
    Article
    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3
    Has Service
    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    Nuclear-localized floxed-mCherry EGFP
    Use
    AAV
    Tags
    Nuclear localization signal
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    March 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    112677-plasmid-map-sequence-id-237606

    Enlarge

  12. pαH-S-GSAS-2P-Omicron-BA.5

    Plasmid
    #213828
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein of OMICRON.2P.BA5 variant
    Depositor
    Priyamvada Acharya
    Article
    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.
    Insert
    pαH-S-GSAS/Omicron-2P-BA5 (S )
    Use
    Tags
    HRV 3C cleavage site (before tags) 8X His tag 2X…
    Expression
    Mammalian
    Mutation
    G339D,R346T,S371F,S373P,S375F,T376A,D405N,R408S,K…
    Promoter
    Available since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    213828-plasmid-map-sequence-id-423246

    Enlarge

  16. pLZRS-ER-Rac1-Q61L-shortlinker

    Plasmid
    #128584
    Purpose
    For mammalian cell expression of Q61L mutant murine RAC1 carrying a modified hormone-binding domain of murine ERalpha at the N-terminus. Activation of expressed latent fusion protein induced by 4-OHT
    Depositor
    Julian Downward
    Article
    Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.
    Insert
    Rac1 (Rac1 Mouse)
    Use
    Retroviral
    Tags
    Modified hormone binding domain of ER alpha
    Expression
    Mammalian
    Mutation
    Changed Glutamine61 to Leucine in the RAC1 moiety.
    Promoter
    Available since
    Aug. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    128584-plasmid-map-sequence-id-254045

    Enlarge

  17. pLZRS-ER-Rac1-P29S-shortlinker

    Plasmid
    #128583
    Purpose
    For mammalian cell expression of P29S mutant murine RAC1 carrying a modified hormone-binding domain of murine ERalpha at the N-terminus. Activation of expressed latent fusion protein induced by 4-OHT
    Depositor
    Julian Downward
    Article
    Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.
    Insert
    Rac1 (Rac1 Mouse)
    Use
    Retroviral
    Tags
    Modified hormone binding domain of ER alpha
    Expression
    Mammalian
    Mutation
    Changed Proline29 to Serine in the RAC1 moiety.
    Promoter
    Available since
    Aug. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    128583-plasmid-map-sequence-id-253120

    Enlarge

  23. pGL3-noSV40-humanEC1.45_min1-2_1XCoordinatorMutant

    Plasmid
    #173963
    Purpose
    Firefly luciferase enhancer reporter plasmid.
    Depositor
    Joanna Wysocka
    Article
    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783
    Insert
    Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with 1 mutated Coordinator motif
    Use
    Luciferase
    Tags
    Expression
    Mutation
    1 mutated Coordinator motif
    Promoter
    SV40 promoter
    Available since
    Sept. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    173963-plasmid-map-sequence-id-341768

    Enlarge

  25. pGG369

    Plasmid
    #165606
    Purpose
    Vector for expression of ω-dCas9 and sgRNA in E. coli: lacUV2-ω-1xFLAG-dCas9(Wt PID)-1xNLS-3xHA-1xNLS and UV5-BsaI-sgRNA (BsaI sites in place of the spacer)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    ω-1xFLAG-dCas9(PAM-interacting domain and overall coding scheme from Wt SpCas9)-1xNLS-3xHA-1xNLS and BsaI-sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    1x FLAG, 3x HA, Omega (ω) subunit of RNA polymera…
    Expression
    Bacterial
    Mutation
    Recoding of BsaI site in the AmpR cassette, two B…
    Promoter
    lacUV2 driving ω-dCas9 and UV5 driving sgRNA
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165606-plasmid-map-sequence-id-330964

    Enlarge

  28. pGL3-noSV40-EC1.45-human_min1-opossum_min2

    Plasmid
    #173966
    Purpose
    Firefly luciferase enhancer reporter plasmid.
    Depositor
    Joanna Wysocka
    Article
    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783
    Insert
    Human minimal enhancer region 1 (min1) and opossum minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45
    Use
    Luciferase
    Tags
    Expression
    Mutation
    See Depositor Comments Below
    Promoter
    SV40 promoter
    Available since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    173966-plasmid-map-sequence-id-341757

    Enlarge

  29. pGL3-noSV40-humanEC1.45_p300peak1-2_7XCoordinatorMutant

    Plasmid
    #173994
    Purpose
    Firefly luciferase enhancer reporter plasmid.
    Depositor
    Joanna Wysocka
    Article
    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783
    Insert
    p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with all 7 Coordinator motifs mutated
    Use
    Luciferase
    Tags
    Expression
    Mutation
    7X Coordinator motif mutation #1-2-3-4-5-6-7
    Promoter
    SV40 promoter
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173994-plasmid-map-sequence-id-341795

    Enlarge

  30. pGL3-noSV40-humanEC1.45_min1-2_4XCoordinatorMutant

    Plasmid
    #173962
    Purpose
    Firefly luciferase enhancer reporter plasmid.
    Depositor
    Joanna Wysocka
    Article
    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783
    Insert
    Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with 4 mutated Coordinator motifs
    Use
    Luciferase
    Tags
    Expression
    Mutation
    4 mutated Coordinator motifs
    Promoter
    SV40 promoter
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173962-plasmid-map-sequence-id-341792

    Enlarge

  34. pGL3-noSV40-humanEC1.45_p300peak1-2_4XCoordinatorMutant#1-2-3-4

    Plasmid
    #173992
    Purpose
    Firefly luciferase enhancer reporter plasmid.
    Depositor
    Joanna Wysocka
    Article
    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783
    Insert
    p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with Coordinator motifs #1, #2, #3 and #4 mutated
    Use
    Luciferase
    Tags
    Expression
    Mutation
    4X Coordinator motif mutations #1-2-3-4
    Promoter
    SV40 promoter
    Available since
    Sept. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    173992-plasmid-map-sequence-id-341764

    Enlarge

  42. pGL3-noSV40-humanEC1.45_p300peak1-2_3XCoordinatorMutant#5-6-7

    Plasmid
    #173993
    Purpose
    Firefly luciferase enhancer reporter plasmid.
    Depositor
    Joanna Wysocka
    Article
    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783
    Insert
    p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with Coordinator motifs #5, #6 and #7 mutated
    Use
    Luciferase
    Tags
    Expression
    Mutation
    3X Coordinator motif mutation #5-6-7
    Promoter
    SV40 promoter
    Available since
    Sept. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    173993-plasmid-map-sequence-id-341706

    Enlarge

  43. pGL3-noSV40-humanEC1.45_min1-2_3XCoordinatorMutant

    Plasmid
    #173964
    Purpose
    Firefly luciferase enhancer reporter plasmid.
    Depositor
    Joanna Wysocka
    Article
    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783
    Insert
    Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with 3 mutated Coordinator motifs
    Use
    Luciferase
    Tags
    Expression
    Mutation
    3 mutated Coordinator motifs
    Promoter
    SV40 promoter
    Available since
    Sept. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    173964-plasmid-map-sequence-id-341641

    Enlarge

  49. pGL4.22-VEGF-HRE::dLUC

    Plasmid
    #128096
    Purpose
    Hypoxia response element real-time luciferase reporter
    Depositor
    Chi Van Dang
    Article
    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:
    Insert
    Hypoxia response element (x5) from VEGF promoter (VEGFA Human)
    Use
    Luciferase
    Tags
    drives expression of destabilized luciferase (2CP…
    Expression
    Mammalian
    Mutation
    Promoter
    inserted VEGF hypoxia response elements (HRE) (x5)
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    128096-plasmid-map-sequence-id-249669

    Enlarge

Showing: 551 - 600 of 626 results