We narrowed to 12,313 results for: 112

1. SLF155: AAV-syn-DO-H2B-jRGECO1a

   Plasmid

   #112278

   Purpose

   For AAV based expression of nuclear jRGECO1a in neurons in Cre-negative neurons with FLEx switch

   Depositor

   Adam Cohen

   Article

   Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

   Insert

   H2B-jRGECO1a
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   May 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. AT-U1

   Plasmid

   #112059

   Purpose

   U1 snRNA tagged with 1 copy of Riboglow (truncated variant A)

   Depositor

   Amy Palmer

   Article

   Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

   Insert

   U1 (RNU1-1 Human)
   

   Expression

   Mammalian

   Mutation

   1 copy of Riboglow tag (truncated variant A) near…

   Available Since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. LUC05 (L/Cys/P)

   Plasmid

   #112146

   Purpose

   Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

   Depositor

   Paul Copeland

   Article

   Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.

   Insert

   Luciferase coding region with wild type Cysteine 258 and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Rat, Photinus pyralis)
   

   Expression

   Mammalian

   Mutation

   none

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. hTPST1

   Plasmid

   #11252

   Depositor

   Hyeryun Choe

   Article

   Choe et al Cell. 2003 Jul 25. 114(2):161-70.

   Insert

   TPST1 (TPST1 Human)
   

   Tags

   C9 (GTETSQVAPA)

   Expression

   Mammalian

   Available Since

   Jan. 19, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pCMV_ABEmax_3xHA

   Plasmid

   #112098

   Purpose

   A:T-to-G:C base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

   Insert

   ABEmax_3xHA
   

   Use

   CRISPR

   Tags

   3xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pExp-TF

   Plasmid

   #112575

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and TF (Trigger factor (tig) from E. coli) fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Tags

   8xHis-TriggerFactor and Strep tag

   Expression

   Bacterial

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST-APLO

   Plasmid

   #112805

   Purpose

   gateway cloning destination vector to express LexAop2 driven transgene in Drosophila

   Depositor

   Chun Han

   Article

   Poe et al Proc Natl Acad Sci U S A. 2017 Sep 19;11

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJW1662

   Plasmid

   #112050

   Purpose

   C-terminal tag with - AID*(AtIAA17_71-114)-HA and OsTIR1 expression cassette marked with LEU

   Depositor

   Jonathan Weissman

   Article

   Costa et al Science. 2018 Jan 18. pii: science.aar36

   Inserts

   IAA17
   TIR1
   

   Tags

   HA

   Expression

   Yeast

   Mutation

   only amino acids 71-114

   Promoter

   ADH1 and None

   Available Since

   Oct. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. mTurquoise2-Rab7(WT)

   Plasmid

   #112959

   Purpose

   In vivo visualization of Rab7 (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   Rab7
   

   Tags

   mTurquoise2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPB-R1R2_EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid

    #112924

    Purpose

    piggyBac transposon vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidin resistant marker

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
    

    Use

    Piggybac transposon vector

    Expression

    Mammalian

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPB-R1R2-EM7neoPheS

    Plasmid

    #112923

    Purpose

    piggyBac Transposon vector with a Gateway destination cassette (neoPheS selection)

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    neoPheS
    

    Use

    Piggybac transposon gateway destination vector

    Expression

    Mammalian

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. EB3-3xmScarletI-N1

    Plasmid

    #112928

    Purpose

    In vivo visualization of microtuble plus ends (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    EB3
    

    Tags

    3x mScarlet-I

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. CD4-C-GFP HDRT Source (pTR 152)

    Plasmid

    #112018

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with GFP

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    CD4-GFP HDRT (CD4 Human)
    

    Use

    CRISPR and Synthetic Biology

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTORGm43GWY

    Plasmid

    #112906

    Purpose

    Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mCitrine.

    Depositor

    Sebastian Schornack

    Article

    Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

    Type

    Empty backbone

    Use

    Oomycete expression

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1(+)-NES-ZapCV2 (cpV143)

    Plasmid

    #112060

    Purpose

    Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)

    Depositor

    Amy Palmer

    Article

    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi

    Insert

    NES-ZapCV2
    

    Tags

    Nuclear Export Signal (NES) derived from HIV-1

    Expression

    Mammalian

    Mutation

    ECFP gene is truncated 36 bp at 3' end. Venu…

    Promoter

    CMV

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. EasyFusion mCherry

    Plasmid

    #112849

    Purpose

    General donor vector with mCherry coding sequence flanking by restriction sites for generating knock-in donor

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Promoter

    No

    Available Since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOP5GP

    Plasmid

    #112610

    Purpose

    Used for expression of protein in E. coli as PreScission cleavable N-terminual 8His and GST tag fusions and with C-terminal Avi-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Tags

    8His-GST and Avi

    Expression

    Bacterial

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV.hSyn-FLEX.iGABASnFR.F102G

    Plasmid

    #112164

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Has Service

    AAV1

    Insert

    iGABASnFR.F102G
    

    Use

    AAV

    Mutation

    F102G

    Promoter

    Synapsin

    Available Since

    July 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. EasyFusion eGFP

    Plasmid

    #112848

    Purpose

    General donor vector with EGFP coding sequence flanking by restriction sites for generating knock-in donor

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    EGFP
    

    Use

    Unspecified; General donor

    Promoter

    No

    Available Since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. ITPKA_3xmScarletI

    Plasmid

    #112960

    Purpose

    In vivo visualization of F-actin (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    ITPKA
    

    Tags

    3x mScarlet-I

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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