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pET-32a(+)-NRG1B1

Plasmid

#120287

Purpose

Produce human recombinant NRG1 in DE3 cells

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

NRG1-beta 1

Tags

6x His and S-Tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 promoter

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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AAVS1 hPGK-PuroR-pA donor

Plasmid

#22072

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

PGK-Puro (PGK1 Human)

Use

Zfn donor

Available Since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pET-32a(+)-TGFB1m

Plasmid

#120286

Purpose

Produce human recombinant mTGFB1 in DE3 cells

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

monomeric Transforming Growth Factor-β1

Tags

6x His and S-Tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 Promotor

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pTRE-TIGHT-EGFP-donor fw copy

Plasmid

#22074

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

TETO-eGFP

Available Since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pC13N-iCAG.copGFP

Plasmid

#66578

Purpose

CLYBL safe harbor gene targeting donor expressing CAG-driven copGFP, compatible with pZT-C13 TALENs

Depositor

Jizhong Zou

Article

Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

Insert

copGFP

Expression

Mammalian

Promoter

CAG

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET-32a(+)-TGFB3m

Plasmid

#120289

Purpose

Produce human recombinant mTGFB3 in DE3 cells

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

monomeric Transforming Growth Factor-β3

Tags

6x His and S-Tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 Promoter

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pmCherry-Synaptojanin-1-145

Plasmid

#223563

Purpose

Expresses the short isoform of SYNJ1 (neuronal) with mCherry tagged at the n-terminus

Depositor

Pietro De Camilli

Article

Mohd Rafiq et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

SYNJ1 (SYNJ1 Human)

Tags

His and mCherry

Expression

Mammalian

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pU6-ACTA2_gRNA1-SpCas9-T2A-GFP

Plasmid

#126706

Purpose

to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human ACTA2 locus at the end of the endogenous coding sequence

Depositor

Darrell Kotton

Article

Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

Insert

ACTA2_gRNA1-SpCas9-T2A-GF (ACTA2 Human)

Use

CRISPR

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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PB-tetO-NGN2

Plasmid

#197090

Purpose

PiggyBac plasmid with tet-inducible expression of transcription factors for cortical neuron differentiation

Depositor

Brian Wainger

Article

Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

Insert

NGN2 (NEUROG2 Human)

Expression

Mammalian

Promoter

TRE3G

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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AAT-PB-CD2APtk

Plasmid

#86004

Purpose

homology donor for AAT locus with piggyBac-flanked DsRedEx_T2A_puroTK cassette

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Insert

DsRedEx_T2A_puro_dTK

Use

Piggybac exciseable

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pET-3c-rhVTN-N

Plasmid

#30225

Depositor

James Thomson

Article

Chen et al Nat Methods. 2011 May;8(5):424-9. doi: 1

Insert

rhVitronectin with N terminus cleavage (VTN Human)

Expression

Bacterial

Mutation

deleted amino acids: 20*-62

Available Since

May 25, 2011

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-TCF21

Plasmid

#192894

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of TCF21

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

TCF21 (TCF21 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pET-3c-rhVTN_FL

Plasmid

#30223

Depositor

James Thomson

Article

Chen et al Nat Methods. 2011 May;8(5):424-9. doi: 1

Insert

rhVitronectin without deletions (VTN Human)

Expression

Bacterial

Mutation

No deletions

Available Since

May 24, 2011

Availability

Academic Institutions and Nonprofits only

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pAAVS1P-iCAG.copGFP

Plasmid

#66577

Purpose

AAVS1 safe harbor gene targeting donor expressing CAG-driven copGFP, compatible with pZT-AAVS1 TALENs

Depositor

Jizhong Zou

Article

Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

Insert

copGFP

Expression

Mammalian

Promoter

CAG

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pLM-mCerulean-cMyc

Plasmid

#23244

Depositor

Michel Sadelain

Article

Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

Insert

mCerulean-2A-MYC (MYC synthetic fluorescent protein, Human)

Use

Lentiviral

Available Since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pLM-mCherry-Klf4

Plasmid

#23243

Depositor

Michel Sadelain

Article

Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

Insert

mCherry_2A_KLF4 (KLF4 synthetic fluorescent protein, Human)

Use

Lentiviral

Available Since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pAAVS1P-iCLHN

Plasmid

#66579

Purpose

AAVS1 safe harbor gene targeting donor expressing CAG-driven Nanoluc-HaloTag, compatible with pZT-AAVS1 TALENs

Depositor

Jizhong Zou

Article

Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

Insert

Nanoluc-HaloTag

Expression

Mammalian

Promoter

CAG

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET-3c-rhVTN-NC

Plasmid

#30226

Depositor

James Thomson

Article

Chen et al Nat Methods. 2011 May;8(5):424-9. doi: 1

Insert

rhVitronectin with N and C terminus cleavages (VTN Human)

Expression

Bacterial

Mutation

deleted amino acids: 399-478, 20-62

Available Since

Sept. 26, 2011

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-TAF4B

Plasmid

#192927

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of TAF4B

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

TAF4B (TAF4B Human)

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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AAT-PB-CG2APtk

Plasmid

#86003

Purpose

homology donor for AAT locus with piggyBac-flanked eGFP_T2A_puroTK cassette

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Insert

eGFP_T2A_puro_dTK

Use

Piggybac exciseable

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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