We narrowed to 23,813 results for: Spr;

1. pSBY3_ligD

   Plasmid

   #104624

   Purpose

   CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD

   Depositor

   Sheng Yang

   Article

   Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

   Insert

   FnCpf1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   FnCpf1 codon-optimized C.glutamicum

   Promoter

   hsp60

   Available since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ154

   Plasmid

   #69305

   Purpose

   Gateway entry vector with AteCas9 (Arabidopsis codon optimized)

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Insert

   AteCas9 (Arabidopsis codon optimized)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   NLS fusion

   Promoter

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330A_dCas9-1x2

   Plasmid

   #63596

   Purpose

   Expresses dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   humanized S. pyogenes dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D1361Y

   Promoter

   CBh

   Available since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCFD4d-U6-1:white1-U6-3:white1

   Plasmid

   #84005

   Purpose

   pCFD4d expresses white sgRNA-1 from a U6:3 promoter and the same white sgRNA-1 from a U6:1 promoter

   Depositor

   Phillip Zamore

   Article

   Ge et al G3 (Bethesda). 2016 Aug 26. pii: g3.116.

   Inserts

   white sgRNA-1
   white sgRNA-1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6:1, U6:3, and dU6-1; dU6-3

   Available since

   Jan. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV_dCas9_VP64

   Plasmid

   #49015

   Purpose

   encodes human-optimized dCas9_VP64 synthetic transcription factor

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Insert

   dCas9_VP64 (human-codon-optimized)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTX171

   Plasmid

   #89258

   Purpose

   Express STU (Single Transcriptional Unit) Cas9 with CaMV 35s promoter in plants

   Depositor

   Daniel Voytas

   Article

   Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOC4 cloning template vector

   Plasmid

   #183423

   Purpose

   FlpON knock-in vector with FLAG-SpCas9 expression

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRISPomyces-Sth1Cas9

   Plasmid

   #129552

   Purpose

   Contains codon-optimized Sth1Cas9 and gRNA for streptomyces

   Depositor

   Mingzi M. Zhang, Fong Tian Wong

   Article

   Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

   Insert

   Sth1Cas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rpsL(XC)-BbsI

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. MLM3639

   Plasmid

   #42252

   Purpose

   Cas9 expression vector

   Depositor

   Keith Joung

   Article

   Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Insert

   Streptococcus pyogenes Cas9-3X Flag
   

   Use

   CRISPR; Zebrafish expression

   Tags

   3X Flag

   Expression

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSMVP-Cas9FL-P2A-turboGFP

    Plasmid

    #80941

    Purpose

    Plasmid that expresses Cas9FL in mammalian cells; co-expresses turboGFP.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9FL
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAN-NOR

    Plasmid

    #62306

    Purpose

    synthetic circuit: NOR gate

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

    Inserts

    A2NT sgRNA
    A2NT sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PPhlF and pBAD

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pColE1_sgRNA_T2_AmpR

    Plasmid

    #119159

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    J23119

    Available since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pColE1_sgRNA_T3_AmpR

    Plasmid

    #119160

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    J23119

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pColE1_sgRNA_T1_AmpR

    Plasmid

    #119158

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    J23119

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSBY2_ligD(FnCpf1)

    Plasmid

    #104623

    Purpose

    CRISPR system used to genome editing in Mycobacterium smegmatis, expresses crRNA to ligD

    Depositor

    Sheng Yang

    Article

    Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

    Insert

    crRNA to ligD
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pj23119

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRE57I-GNAc

    Plasmid

    #170637

    Purpose

    DNA donor for transposition

    Depositor

    Sheng Yang

    Article

    Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

    Insert

    Mini-Tn6677, GNAc cassette, lacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. MiniCoopR U6:gRNA, mitfa:Cas9

    Plasmid

    #118840

    Purpose

    Targets a gene and expresses zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Type

    Empty backbone

    Use

    CRISPR; Tol2

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCCM935

    Plasmid

    #58202

    Purpose

    Expresses unc-22 sgRNA in C.elegans. Can be used in Co-CRISPR experiments.

    Depositor

    Craig Mello

    Article

    Kim et al Genetics. 2014 May 30. pii: genetics.114

    Insert

    unc-22 sgRNA
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available since

    July 30, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pMB66

    Plasmid

    #47946

    Purpose

    Expresses C. elegans optimized Cas9::mEGFP from the hsp-16.48 promoter

    Depositor

    Mike Boxem

    Article

    Waaijers et al Genetics. 2013 Aug 26.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, egl-13 NLS, and mEGFP

    Expression

    Worm

    Mutation

    Promoter

    hsp-16.48

    Available since

    Dec. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. EC59103

    Plasmid

    #191785

    Purpose

    LbCas12a expression in plants

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    AtUbi10::Cas12a+ 8 introns-T-E9
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D156R

    Promoter

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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