We narrowed to 10,326 results for: ada
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Plasmid#185274PurposeFor the mammalian expression of the human protein APOE_HUMAN_E114I. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertAPOE_HUMAN_E114I
ExpressionMammalianMutationE114IAvailable SinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_ELK3
Plasmid#167750PurposeExpresses ELK3 in mammalian cellsDepositorAvailable SinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_OTmut
Plasmid#185385PurposeExpresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in mammalian cellsDepositorInsertGPCR activation based oxytocin control sensor GRAB_OTmut
ExpressionMammalianPromoterCMVAvailable SinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-hFIP200(1363-1370Mut)-MBP
Plasmid#190038PurposeExpression of FIP200 mutantDepositorInsertFIP200 (RB1CC1 Human)
TagsGST and MBPExpressionMammalianMutation1363 RDKDLIES 1370 to GSSGGSSGPromoterCMVAvailable SinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-N-mSOG-bFos-IRES-mCherry
Plasmid#136599PurposeEncodes bFos domain tagged with the N-terminal fragment of split-miniSOG (mSOG1-94).DepositorInsertN-mSOG-bFos
ExpressionMammalianPromoterCMVAvailable SinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IPU-FLAG-NEK9T210A
Plasmid#168270PurposeExpresses kinase-dead NEK9 mutant in mammalian cellsDepositorAvailable SinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAVA-Gal11p-LGF2
Plasmid#127480PurposePositive control for the AVA-seq system. Gal11p (lambda CI associated) and LGF2 (RNAp associated) interactionDepositorInsertGal11p-LGF2 (GAL11 Budding Yeast)
ExpressionBacterialAvailable SinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAV10.K5
Plasmid#63210PurposepCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceFeb. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV10.K4
Plasmid#63212PurposepCACS backbone (Amp), TRP1, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into chrIXR: 387328-388330.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceFeb. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCold I-Hero7
Plasmid#187926PurposeBacterial expression of heat-resistant obscure (Hero) protein Hero7DepositorAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1 puro-luc (shRNA)
Plasmid#153962PurposeExpresses a shRNA against firefly luciferaseDepositorInsertshRNA against firefly luciferase (19-bp-long target)
UseLuciferase, RNAi, and RetroviralTagsN/AExpressionMammalianPromoterU6 promoterAvailable SinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mEGFP-mTrim21ΔPRYSPRY
Plasmid#105521PurposeT7 promotor drives in vitro transcription of mEGFP-tagged mouse TRIM21 with deleted PRYSPRY domain mRNADepositorInsertTRIM21 (Trim21 Mouse)
UseIn vitro transcriptionTagsmEGFPMutationdeletion of PRYSPRY domain (aa1-281)PromoterT7Available SinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
3xFN-Tel-TAL/pCMV-7.1
Plasmid#63589PurposeExpresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat.DepositorInsert3xFVN-Tel-TAL
UseTALENTags3xFLAG-tag, V5-tag, and nuclear localization sign…ExpressionMammalianPromoterCMVAvailable SinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST_AfrLEA3m (pBS0911)
Plasmid#185285PurposeFor the mammalian expression of the brine shrimp protein AfrLEA3m. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertAfrLEA3m
ExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST_PvLEA4_repeats_k3_26 (pBS0762)
Plasmid#185244PurposeFor the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k3_26. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertPvLEA4_repeats_k3_26
ExpressionMammalianAvailable SinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCI-TPI_PTC160-xrRNA-4H
Plasmid#108369PurposeExpresses TPI reporter with premature termination codon (PTC160); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertPTC160 TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
ExpressionMammalianMutationPTC160PromoterCMVAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEX-YFP-hLC3ΔG
Plasmid#24990DepositorInserta mutant human MAP1-LC3B fused with EYFP (MAP1LC3B Human)
TagsEYFPExpressionMammalianMutationdeleted Gly at 121Available SinceJune 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2441 - [1-2] ENTR - Fluor - ce-Dendra2(PATCs(900bp), NLS, no_atg, no stop)
Plasmid#159870PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-Dendra2(PATCs(900bp), NLS, no_atg, no stop)
ExpressionWormMutationNot applicableAvailable SinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
7749 Bicistronic_ires_hygro
Plasmid#64374PurposeThis a retroviral expression plasmid with a minimal polylinker followed by IRES and hygro resistance geneDepositorTypeEmpty backboneUseRetroviralAvailable SinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR RPS27A K113R-3xFlag_IRES-GFP
Plasmid#198391PurposeRPS27A K113R (ubiquitin/ribosomal protein eS31 fusion) expressionDepositorInsertRPS27A (RPS27A Human)
UseLentiviralTags3xFlagExpressionMammalianMutationubiquitination site mutant (K113R)PromoterEF1AAvailable SinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRvCAHS1-mEGFP-NLS
Plasmid#205013PurposeThe vector contains 1kbp of the upstream and downstream regions of the cahs1 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the cahs1 gene from Ramazzottius varieornatus
UseTardigrade expressionAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRvSAHS1-mCherry-NLS
Plasmid#205009PurposeThe vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the mCherry gene with NLS sequence positioned in between.DepositorInsertmCherry-NLS flanked by 1kbp upstream and downstream of the sahs1 gene from R. varieornatus
UseTardigrade expressionAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR v2-sgAMPK alpha1 clone2
Plasmid#162120PurposeLentiviral sgRNA plasmid targeting human AMPK alpha1DepositorInsertsgAMPK alpha1 (PRKAA1 Human)
UseLentiviralAvailable SinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
p7612 MSCV-P C-FlagHA 16E7 E10K
Plasmid#163303PurposeExpresses HPV16 E7 E10KDepositorInsertHPV16 E7 E10K
UseRetroviralTagsFlagHAMutationE10KPromoterMSCV LTRAvailable SinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
-111 hTF m2
Plasmid#15449DepositorInsertTissue factor promoter (F3 Human)
UseLuciferaseExpressionMammalianMutationAll 3 Sp1 sites mutatedAvailable SinceAug. 31, 2007AvailabilityAcademic Institutions and Nonprofits only -
Intersectin I SH3 B domain (human)
Plasmid#47414DepositorInsertIntersectin SH3 domain B (ITSN1 Human)
TagsGSTExpressionBacterialMutationonly SH3 domain BPromotertacAvailable SinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2443 - [1-2] ENTR - Fluor - ce-mMaple3(PATCs(900bp), NLS, no_atg, no stop)
Plasmid#159867PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-mMaple3(PATCs(900bp), NLS, no_atg, no stop)
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2309 - [1-2] ENTR - Fluor - mCardinal(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
Plasmid#159861PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - mCardinal(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
shCTL-mlpx-puro
Plasmid#65232Purposeencodes a non-targeting control shRNADepositorInsertnon-coding shRNA
ExpressionMammalianPromoterPGKAvailable SinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST_SERF2_HUMAN (pBS0751)
Plasmid#185239PurposeFor the mammalian expression of the human protein SERF2_HUMAN. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertSERF2_HUMAN
ExpressionMammalianAvailable SinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNAZeo(-)HSC73AS
Plasmid#86031PurposeAntisense for pCDNAZeo(-)HSC73DepositorAvailable SinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-RIPK3 2KR(55,363)
Plasmid#78811PurposeTo overexpress RIPK3 2KR(55,363) in Mammalian CellsDepositorAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
PRK5-FLAG-USP13/5-4
Plasmid#61746Purposeexpress N-terminally FLAG tagged human chimera USP13/5-4DepositorTagsFLAGExpressionMammalianMutationUSP5 (1-716aa) fused to USP13 (722-864aa)PromoterCMVAvailable SinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
PRK5-FLAG-USP13/5-3
Plasmid#61745Purposeexpress N-terminally FLAG tagged human chimera USP13/5-3DepositorTagsFLAGExpressionMammalianMutationUSP5 (1-628aa) fused to USP13 (653-864aa)PromoterCMVAvailable SinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
PRK5-FLAG-USP13/5-1
Plasmid#61743Purposeexpress N-terminally FLAG tagged human chimera USP13/5-1DepositorTagsFLAGExpressionMammalianMutationUSP5 (1-185aa) fused to USP13 (197-864aa)PromoterCMVAvailable SinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pONSY-mCherry
Plasmid#111874PurposeThis plasmid expresses mCherry fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.DepositorInsertmCherry
UseCapsaspora owczarzakiTagsmCherryPromoterElongation Factor 1 alpha (EF1a) from CapsasporaAvailable SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1 UBXN11
Plasmid#169017PurposeExpresses GST-UBXN11 (human) in bacteriaDepositorAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pST_MAHS_RAMVA (pBS0310)
Plasmid#185169PurposeFor the mammalian expression of the tardigrade protein MAHS_RAMVA. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertMAHS_RAMVA
ExpressionMammalianAvailable SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZA1
Plasmid#158479PurposeEntry clone for Golden Gate assembly. Golden Gate compatible wild-type A. thaliana ZAR1 with STOP codonDepositorAvailable SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-UBXD1-Strep-HA
Plasmid#113476PurposeExpression of human UBXD1 with C-terminal strep-HA tagDepositorInsertUBXD1 (UBXN6 Human)
Tags2xstrep-HAExpressionMammalianMutationC174T silent mutation, wildtype without stop codonPromoterCMVAvailable SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only