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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,065 results for: SPL;

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Showing: 2421 - 2440 of 4065 results

  8. pM162

    Plasmid
    #173221
    Purpose
    Expresses ScFV-GCN4-GFP10M2-GB1-T2A-OptGFP(1-9)-GB1 and Puro-T2A-MCP-mCherry-GFP11-GB1
    Depositor
    Hajin Kim
    Article
    Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do
    Inserts
    ScFV-GCN4-GFP10M2-GB1-T2A-OptGFP (1-10)-GB1
    MCP-mCherry-GFP11-GB1
    Use
    CRISPR and Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    173221-plasmid-map-sequence-id-341549

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  9. gRNA-CasRx_SD1-pSV40-TagBFP

    Plasmid
    #221004
    Purpose
    Transiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.
    Depositor
    Benjamin Blencowe, Mikko Taipale
    Article
    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e
    Insert
    CasRx 30nt processed direct repeat with SD1 mutation
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    DR1 A>T
    Promoter
    U6
    Available Since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    221004-plasmid-map-sequence-id-436033

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  11. TfR-sfGFP-myc tag-SpyCatcher003

    Plasmid
    #133451
    Purpose
    Expresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detection
    Depositor
    Mark Howarth
    Article
    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p
    Insert
    TfR-sfGFP-myc tag-SpyCatcher003
    Tags
    GSSGS and myc tag
    Expression
    Mammalian
    Mutation
    Contains C20 and A23 mutations that improve plasm…
    Promoter
    CMV
    Available Since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    133451-plasmid-map-sequence-id-267048

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  19. pEVOL-NBK-1

    Plasmid
    #207639
    Purpose
    A plasmid for the expression of M. mazei pyrrolysine aminoacyl-tRNA synthetase/tRNA pair (PylRS/tRNAPyl) in E. Coli.
    Depositor
    Cole DeForest
    Article
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Insert
    Pyl-tRNACUA and 2x pyrrolysyl-tRNA synthetase (MmPylRS)
    Expression
    Bacterial
    Mutation
    The evolved synthetase NBK-1 contains mutations Y…
    Promoter
    araBAD
    Available Since
    Oct. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    207639-plasmid-map-sequence-id-409890

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Showing: 2421 - 2440 of 4065 results