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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 25,186 results for: Spr;

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Showing: 2461 - 2480 of 25186 results

  1. pCMV_dCas9_VP64

    Plasmid
    #49015
    Purpose
    encodes human-optimized dCas9_VP64 synthetic transcription factor
    Depositor
    Timothy Lu
    Article
    Farzadfard et al ACS Synth Biol. 2013 Sep 11.
    Insert
    dCas9_VP64 (human-codon-optimized)
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    49015-plasmid-map-sequence-id-79086

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  2. pSBY3_ligD

    Plasmid
    #104624
    Purpose
    CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD
    Depositor
    Sheng Yang
    Article
    Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d
    Insert
    FnCpf1
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    FnCpf1 codon-optimized C.glutamicum
    Promoter
    hsp60
    Available Since
    June 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    104624-plasmid-map-sequence-id-201663

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  4. pYPQAT03

    Plasmid
    #223375
    Purpose
    T-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-SpCas9-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223375-plasmid-map-sequence-id-452219

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  14. pMB66

    Plasmid
    #47946
    Purpose
    Expresses C. elegans optimized Cas9::mEGFP from the hsp-16.48 promoter
    Depositor
    Mike Boxem
    Article
    Waaijers et al Genetics. 2013 Aug 26.
    Insert
    Cas9
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, egl-13 NLS, and mEGFP
    Expression
    Worm
    Promoter
    hsp-16.48
    Available Since
    Dec. 6, 2013
    Availability
    Academic Institutions and Nonprofits only
    47946-plasmid-map-sequence-id-68806

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  18. pAEF5

    Plasmid
    #136305
    Purpose
    Plasmid encoding the Cas9 gene and a gRNA expression cassette allowing to clone a single or multiple gRNAs for DSBs induction in yeast. Constructed by Aubin Fleiss
    Depositor
    Gilles Fischer
    Article
    Agier et al Methods Mol Biol. 2021;2196:181-198. doi
    Type
    Empty backbone
    Use
    CRISPR
    Available Since
    Feb. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    136305-plasmid-map-sequence-id-271550

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Showing: 2461 - 2480 of 25186 results