164,060 results
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Plasmid#187924PurposeExpresses Luciferase reporter RNA construct under the 5' and 3' UTRs of NA segment of Influenza B/Brisbane/60/2008 virus by RNA polymerase I in mammalian cellsDepositorInsertReporter RNA (RNA encoding firefly luciferase under the viral promoter of segment 6 of Influenza B/Brisbane/60/2008 virus)
UseTagsExpressionMammalianMutationPromoterRNA polymease I PromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLIK_hygro_BirA
Plasmid#136537PurposeLentiviral expression vector for an inducible BirADepositorInsertBirA
UseLentiviral; Destinatioin vector for gateway cloni…TagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-OMM-GCaMP6f
Plasmid#127874PurposeFluorescent reporter for mitochondrial outer membrane Ca2+DepositorInsertOuter membrane localized GCaMP6f
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
AID* Kit
Plasmid Kit#1000000120PurposeExpanded tool kit for the auxin-inducible degron (AID) system in yeast ( S. cerevisiae). Includes tags for easy detection by antibodies/microscopy and a set of selection markers.DepositorAvailable sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dLight1.5
Plasmid#111058PurposeExpresses dLight1.5 in mammalian cellsDepositorInsertdLight1.5
UseTagsFlag tagExpressionMammalianMutationPromoterAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMT85_PS-mEos3.2_GentaR
Plasmid#173895PurposeTransposon allowing the expression of the fluorescent protein mEos3.2 in multiple mycoplasma species, under the control of the Spiralin promoterDepositorInsertmEos3.2
UseTagsExpressionBacterialMutationPromoterSpiralinAvailable sinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPICZaa-STE2-cohis
Plasmid#133433PurposeYeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.DepositorInsertSTE2 (STE2 Budding Yeast)
UseTagsExpressionYeastMutationC59S/C252A, Codon optimized for P. pastoris, and …PromoterAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P29
Plasmid#120040PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Strep-IIDepositorInsertStrep-II
UsePcr templateTagsStrep-IIExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF0335
Plasmid#144358PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF91 (ZNF91 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKI1.1R
Plasmid#85808PurposeCRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.DepositorInserthuman-codon-optimized SpCas9
UseCRISPRTagsFLAG and NLSExpressionPlantMutationPromoterAtRPS5AAvailable sinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHM6-alphasynuclein-WT
Plasmid#40824DepositorInsertSNCA (SNCA Human)
UseTags6x His and HAExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
P2A-NLS-tdtomato-EPG backbone
Plasmid#215548PurposeAn empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of puromycin-EGFPDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
M77 pIRESpuro-GLUE-hDsh3
Plasmid#15102DepositorInsertDsh3 (DVL3 Human)
UseTagsCBP tag, HA, and streptagExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HshnRNPH1_Z
Plasmid#148120PurposeMammalian Expression of HshnRNPH1DepositorInsertHshnRNPH1 (HNRNPH1 Human)
UseTagsExpressionMammalianMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
V5-ERG-R367K
Plasmid#118624PurposeExpresses V5 tagged ERG R367K (DNA binding mutant) proteinDepositorInsertERG (R367K DNA binding mutant) (ERG Human)
UseLentiviralTagsV5ExpressionMammalianMutationR367KPromoterEF1aAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-sgRNA
Plasmid#71409PurposeThere is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBIDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT85_P438-mEOS3.2_GentaR
Plasmid#173894PurposeTransposon allowing the expression of the fluorescent protein mEos3.2 in multiple mycoplasma species, under the control of the P438 promoterDepositorInsertmEos3.2
UseTagsExpressionBacterialMutationPromoterP438Available sinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTSlb-wt
Plasmid#60728PurposeExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD.DepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…PromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
p2xFlag CMV2-YAP2-1st WW mutant
Plasmid#19046DepositorInsertYes-kinase associated protein isoform 3 (YAP1 Human)
UseTags2xFlagExpressionMammalianMutationTryptophan 199 changed to Alanine,and Proline 202…PromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEGFP VAMP7 (102-220)
Plasmid#42314DepositorInsertVAMP7 Coiled Coil domain (102-220) (VAMP7 Human)
UseTagsEGFPExpressionMammalianMutationΔ1-102PromoterCMVAvailable sinceMarch 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
p2xFlag CMV2-YAP2-2nd WW mutant
Plasmid#19047DepositorInsertYes-kinase associated protein (YAP1 Human)
UseTags2xFlagExpressionMammalianMutationTryptophan 258 changed to Alanine, and Proline 2…PromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJSK659
Plasmid#205128PurposeExpression of GFP-SnxA in mammalian cellsDepositorInsertSnxA
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
P2A-mCherry-EhT backbone
Plasmid#215549PurposeAn empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of hygromycin-NLS-tdTomatoDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
PL452-hGH-polyA-PGK-neoR
Plasmid#191681PurposeDonor plasmid backbone PGK-neoRDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationWTPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO hChR2(H134R)-EYFP (AAV Retrograde)
Viral Prep#55639-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-Ef1a-fDIO hChR2(H134R)-EYFP (#55639). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO hChR2(H134R)-EYFP plasmid DNA. Ef1a-driven, Flp-dependent expression of ChR2(H134R)-EYFP. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsEYFPAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
5XUAS IVS CsChrimson tdtomato_tr
Plasmid#111545PurposeOptogenetics in Drosophila: UAS-induced expression of Chrimson tdTomatoDepositorInsertCsChrimson_tdtomato_trafficked
UseTagsExpressionInsectMutationwtPromoterhsp70Available sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
3CEG
Plasmid#60107PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertBIRC6:SDC136-F10:C41228 (BIRC6 Human)
UseTags6xHisExpressionBacterialMutationSee commentsPromoterT7Available sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
SF-SON IRES Puro
Plasmid#214402PurposeExpresses human SOX10, OLIG2, NKX6.2 and PAC (puromycin N-acetyl-transferase)DepositorUseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF1782
Plasmid#143315PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF239 (ZNF239 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL1200
Plasmid#138526PurposeFor expresson of mlaFEDCB operon from E. coli. N-terminal his tag on MlaD, araBAD promoter.DepositorUseTagsN-terminal His tag and TEV cleavage site on MlaDExpressionBacterialMutationPromoteraraBADAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only