161,697 results

1. pCMV-MMLVgag-3xNES-ABE7.10-NG

   Plasmid

   #181753

   Purpose

   MMLV-Gag fused to 3xNES-ABE7.10-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

   Depositor

   David Liu

   Article

   Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

   Insert

   MMLVgag-3xNES-ABE7.10-NG
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. SpCas9 TadCBEd-V106W

   Plasmid

   #193839

   Purpose

   Express TadCBEd-V106W (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadA-CDd V106W-SpCas9 D10A-2xUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLVX-NLS-HA-TurboID

   Plasmid

   #215075

   Purpose

   Expresses NLS-HA-TurboID in mammalian cells from a lentiviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   TurboID (birA )
   

   Use

   Lentiviral

   Tags

   NLS-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJSK659

   Plasmid

   #205128

   Purpose

   Expression of GFP-SnxA in mammalian cells

   Depositor

   Jason King

   Article

   Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

   Insert

   SnxA
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEGFP-LC3

   Plasmid

   #21073

   Purpose

   Mammalian expression of rat LC3 fused to EGFP

   Depositor

   Tamotsu Yoshimori

   Article

   Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   First ATG is not included.

   Promoter

   CMV

   Available since

   May 11, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEP4 E02S EN2L

   Plasmid

   #20922

   Purpose

   used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Nanog and Lin28

   Depositor

   James Thomson

   Article

   Yu et al Science. 2009 Mar 26.

   Insert

   Oct4 and Sox2; Nanog and Lin28 (POU5F1, SOX2, NANOG, LIN28A Human)
   

   Use

   Episomal/ebna

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 20, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Mouse Chromatin Regulator Library

   Pooled Library

   #200011

   Purpose

   CRISPR KO library targeting a comprehensive set of genes with annotated function as chromatin regulators.

   Depositor

   Bradley Bernstein, Gabriel Griffin, Robert Manguso

   Article

   Griffin et al Nature. 2021 Jul;595(7866):309-314. doi:

   Expression

   Mammalian

   Use

   CRISPR and Lentiviral

   Available since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

8. pGP-AAV-syn-jGCaMP7s-WPRE (AAV9)

   Viral Prep

   #104487-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pGP-AAV-syn-jGCaMP7s-WPRE (#104487). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP7s-WPRE plasmid DNA. Synapsin-driven GCaMP7s calcium sensor. These AAV preparations are suitable purity for injection into animals.

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Promoter

   Syn

   Tags

   None

   Available since

   Aug. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

9. pLenti6.3-hEPO

   Plasmid

   #50436

   Purpose

   express human erythropoietin using lentiviral delivery system

   Depositor

   Juan Melero-Martin

   Article

   Lin et al Blood. 2011 Nov 17;118(20):5420-8. doi:

   Insert

   EPO (EPO Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

10. H3K9me3 biosensor

    Plasmid

    #120802

    Purpose

    FRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRET

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Truncated YPet-HP1-EV linker-ECFP-mouse histone H3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2

    Plasmid

    #64512

    Purpose

    Expression of St1 dCas9-3XTagBFP2 in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XmTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pIDK-HDAC1fl

    Plasmid

    #109158

    Purpose

    Encodes human full-length HDAC1 to be expressed in a baculovirus/insect cell expression system

    Depositor

    Monika Golas

    Article

    Inui et al Protein Sci. 2017 May;26(5):997-1011. do

    Insert

    human full-length HDAC1, sequence-optimized for insect cells (HDAC1 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    p10

    Available since

    Jan. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Flag-hCRM1

    Plasmid

    #17647

    Depositor

    Xin Wang

    Article

    Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

    Insert

    CRM1 (XPO1 Human)
    

    Use

    Tags

    3x Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-endo-Nb37

    Plasmid

    #205514

    Purpose

    Endosome-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.

    Depositor

    Jin Zhang

    Article

    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

    Insert

    endo-Nb37
    

    Use

    Tags

    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-ICUE3

    Plasmid

    #61622

    Purpose

    FRET-based biosensor for monitoring cAMP

    Depositor

    Jin Zhang

    Article

    DiPilato et al Mol Biosyst. 2009 Aug;5(8):832-7. doi: 1

    Insert

    ICUE3 (RAPGEF3 Human)
    

    Use

    Tags

    ECFP and cpVenus[L194]

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-Ef1a-Coff/Fon-sRGECO (AAV8)

    Viral Prep

    #137128-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Coff/Fon-sRGECO (#137128). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Coff/Fon-sRGECO plasmid DNA. EF1a-driven, Flp-dependent expression of cytosolic sRGECO calcium sensor (inhibited in presence of Cre). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    EF1a

    Tags

    None

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

17. pAAV-Ef1a-DIO EYFP (AAV9)

    Viral Prep

    #27056-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-Ef1a-DIO EYFP (#27056). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO EYFP plasmid DNA. EF1a-driven, cre-dependent EYFP expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    Tags

    EYFP (Cre-dependent)

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

18. LAMP1-RpHLuorin2

    Plasmid

    #171720

    Purpose

    Lysosomal expression of ratiometric pHLuorin2

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    Ratiometric pHLuorin2 fused to LAMP1 (LAMP1 Human)
    

    Use

    Tags

    ratiometric pHluorin2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEGFPc1 IP3R3 WT

    Plasmid

    #236453

    Purpose

    transient overexpression of IP3R3 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Kuchay et al Nature. 2017 Jun 22;546(7659):554-558. d

    Insert

    IP3R3 (Itpr3 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGP-CMV-jGCaMP8m

    Plasmid

    #162372

    Purpose

    Mammalian expression of ultrafast protein calcium sensor

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8m
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    A25G F286Y

    Promoter

    CMV

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLentiCMV Puro DEST ERKKTRClover

    Plasmid

    #59150

    Purpose

    Lentiviral vector to express ERK KTR mClover under CMV promoter (With Puromycin Resistance)

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    ERK Kinase Translocation Reporter
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. iBE4max YE1 NG CLYBL

    Plasmid

    #174570

    Purpose

    doxycycline inducible BE4max YE1 NG expression for human CLYBL locus knock-in

    Depositor

    Mathew Garnett

    Article

    Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

    Insert

    BE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tet ON

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDuet TDP-43 Q331K

    Plasmid

    #27465

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Coexpression

    Tags

    Expression

    Bacterial

    Mutation

    Glutamine 331 mutated to Lysine (Q331K)

    Promoter

    Available since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGW_GPHN.FingR-YFP

    Plasmid

    #46298

    Depositor

    Don Arnold

    Article

    Gross et al Neuron. 2013 Jun 19;78(6):971-85. doi: 1

    Insert

    GPHN.FingR (GPHN Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CMV-mito-Citron1

    Plasmid

    #134305

    Purpose

    mitochondria-targeted direct-response citrate sensor

    Depositor

    Robert Campbell

    Article

    Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

    Insert

    Citron1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFB1.HMBP.PrS.EZH2

    Plasmid

    #125161

    Purpose

    Expresses human EZH2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Baculovirus expression vector

    Tags

    His-MBP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28-hTDG

    Plasmid

    #70758

    Purpose

    bacterial expression of human TDG

    Depositor

    Primo Schaer

    Article

    Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

    Insert

    TDG (TDG Human)
    

    Use

    Tags

    6-HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7/lac operon

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA5/FRT/TO_H2B-HaloTag10-T2A-Tomm20-HaloTag9

    Plasmid

    #175525

    Purpose

    Co-expression of HaloTag10 (nuclear) and 9 (mitochondrial surface) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag10 and Tomm20-HaloTag9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag10 = HaloTag7-Q165W and HaloTag9 = HaloTag…

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFB-EPCP1

    Plasmid

    #226783

    Purpose

    Expression of Z-tagged LRRK2 RCKW using Baculoviruses

    Depositor

    Sebastian Mathea

    Article

    Dederer et al J Biol Chem. 2024 Jul;300(7):107469. doi

    Insert

    Leucine-rich repeat kinase 2 (LRRK2 Human)
    

    Use

    Tags

    His-Z-TEV

    Expression

    Insect

    Mutation

    Amino acids deleted 1-1326

    Promoter

    Available since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTn7-SCOUT12.G

    Plasmid

    #201000

    Purpose

    pLv1-pUC18R6KT-miniTn7-Km-sfGFP, KmR, ApR

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Insert

    superfolder GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23104

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge