We narrowed to 13,812 results for: cas9 genes

1. PM-SP!TB

   Plasmid

   #48650

   Purpose

   Bacterial SP crRNA expression: targets SP to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   Bacterial SP crRNA to prototspacer B
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   J23100 promoter

   Available since

   Oct. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG-TetR-Rad18UBD

   Plasmid

   #119010

   Purpose

   Mammalian expression of a fusion protein of single chain Tet repressor with the human Rad18 Ubiquitin binding domain and BFP expression

   Depositor

   Ralf Kuehn

   Article

   Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

   Insert

   TetR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAG-Trex2-bpA

   Plasmid

   #86984

   Purpose

   Expression of mouse Trex2

   Depositor

   Ralf Kuehn

   Article

   Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

   Insert

   mouse Trex2 coding region (Trex2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAS_gpyrG1

   Plasmid

   #90276

   Purpose

   (also pMST621-BB3_gpyrG1_cas9) CRISPR/Cas9 plasmid with gRNA for pyrG1, Cas9

   Depositor

   Michael Sauer

   Article

   Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

   Insert

   gRNA (pyrg1)
   

   Use

   A. niger

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMLS234

   Plasmid

   #73682

   Purpose

   SapTrap 3-site Destination vector for N-terminal GFP tagging with embedded Cbr-unc-119 selectable marker

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   GFP + Cbr-unc-119
   

   Use

   CRISPR and Cre/Lox

   Tags

   GFP

   Expression

   Worm

   Mutation

   Promoter

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PM-TD!TB

   Plasmid

   #48656

   Purpose

   Bacterial TD crRNA expression: targets TD to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   Bacterial TD crRNA to prototspacer B
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   J23100 promoter

   Available since

   Oct. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pX330_HR_Prnp_3

   Plasmid

   #78621

   Purpose

   pX330 vector encoding SpCas9 and a chimeric guide RNA targeting Prnp coding sequence

   Depositor

   Walker Jackson

   Article

   Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

   Insert

   pX330_HR_Prnp_3
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAG-TetR-RNF169UBD

   Plasmid

   #119011

   Purpose

   Mammalian expression of a fusion protein of single chain Tet repressor with the human RNF169 Ubiquitin binding domain and BFP expression

   Depositor

   Ralf Kuehn

   Article

   Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

   Insert

   TetR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. SK-YFP-TD-B

   Plasmid

   #48663

   Purpose

   Bacterial TD repression YFP reporter: protospacer B

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   TD prototspacer B/PAM with reporter EYFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   lambda pR promoter

   Available since

   Oct. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SK-YFP-ST1-A

    Plasmid

    #48665

    Purpose

    Bacterial ST1 repression YFP reporter: protospacer A

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    ST1 prototspacer A/PAM with reporter EYFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    lambda pR promoter

    Available since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PM-NM!TA

    Plasmid

    #48651

    Purpose

    Bacterial NM crRNA expression: targets NM to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Bacterial NM crRNA to prototspacer A
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    J23100 promoter

    Available since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PM-TD!TA

    Plasmid

    #48655

    Purpose

    Bacterial TD crRNA expression: targets TD to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Bacterial TD crRNA to prototspacer A
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    J23100 promoter

    Available since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PM-ST1!TB

    Plasmid

    #48654

    Purpose

    Bacterial ST1 crRNA expression: targets ST1 to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Bacterial ST1 crRNA to prototspacer B
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    J23100 promoter

    Available since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PM-NM!TB

    Plasmid

    #48652

    Purpose

    Bacterial NM crRNA expression: targets NM to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Bacterial NM crRNA to prototspacer B
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    J23100 promoter

    Available since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PM-SP!TA

    Plasmid

    #48649

    Purpose

    Bacterial SP crRNA expression: targets SP to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Bacterial SP crRNA to prototspacer A
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    J23100 promoter

    Available since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SK-YFP-ST1-B

    Plasmid

    #48662

    Purpose

    Bacterial ST1 repression YFP reporter: protospacer B

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    ST1 prototspacer B/PAM with reporter EYFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    lambda pR promoter

    Available since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PM-ST1!TA

    Plasmid

    #48653

    Purpose

    Bacterial ST1 crRNA expression: targets ST1 to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Bacterial ST1 crRNA to prototspacer A
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    J23100 promoter

    Available since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. L1p6_barley_sgRNA_scaffold

    Plasmid

    #231033

    Purpose

    Cloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 6

    Depositor

    Wilma van Esse

    Article

    Winkelmolen et al bioRxiv 2024.08.22.609225

    Insert

    Scaffold for Cas9 mediated CRISPR
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TaU6

    Available since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. L1p5_barley_sgRNA_scaffold

    Plasmid

    #231032

    Purpose

    Cloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 5

    Depositor

    Wilma van Esse

    Article

    Winkelmolen et al bioRxiv 2024.08.22.609225

    Insert

    Scaffold for Cas9 mediated CRISPR
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TaU6

    Available since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. L1p4_barley_sgRNA_scaffold

    Plasmid

    #231031

    Purpose

    Cloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 4

    Depositor

    Wilma van Esse

    Article

    Winkelmolen et al bioRxiv 2024.08.22.609225

    Insert

    Scaffold for Cas9 mediated CRISPR
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TaU6

    Available since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. L1p3_barley_sgRNA_scaffold

    Plasmid

    #231030

    Purpose

    Cloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 3

    Depositor

    Wilma van Esse

    Article

    Winkelmolen et al bioRxiv 2024.08.22.609225

    Insert

    Scaffold for Cas9 mediated CRISPR
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TaU6

    Available since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSCL194

    Plasmid

    #222225

    Purpose

    Expresses site 1/Cas9/Eco1 RT in HIS3 locus.

    Depositor

    Seth Shipman

    Article

    Crawford et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

    Insert

    site 1/Cas9/Eco1 RT
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSCL195

    Plasmid

    #222226

    Purpose

    Expresses site 2/Cas9/Eco1 RT in HIS3 locus.

    Depositor

    Seth Shipman

    Article

    Crawford et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

    Insert

    site 2/Cas9/Eco1 RT
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSCL368

    Plasmid

    #222227

    Purpose

    Expresses site 3/Cas9/Eco1 RT in HIS3 locus.

    Depositor

    Seth Shipman

    Article

    Crawford et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

    Insert

    site 3/Cas9/Eco1 RT
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMLS1272

    Plasmid

    #188891

    Purpose

    Ric-4 sgRNA expression vector

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    PU6(R07E5.16)::Ric-4 sgRNA (CELE_Y22F5A.3 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMLS715

    Plasmid

    #188887

    Purpose

    Multisite Gateway [4-3] Destination vector; lox2272-flanked Cbr-unc-119 marker; homology to 5' miniMos Arm

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Type

    Empty backbone

    Use

    Multisite gateway [4-3] destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PV384

    Plasmid

    #132344

    Purpose

    Cas9 DsRed targeting sgRNA expression cassette for Zea mays

    Depositor

    Joshua Young

    Article

    Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

    Insert

    Cas9 DsRed targeting sgRNA
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAS_gpyrG2

    Plasmid

    #90277

    Purpose

    (also pMST620-BB3_gpyrG2_cas9) CRISPR/Cas9 plasmid with gRNA for site pyrG2, Cas9

    Depositor

    Michael Sauer

    Article

    Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

    Insert

    gRNA (pyrg2)
    

    Use

    A. niger

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. SK-YFP-NM-A

    Plasmid

    #48664

    Purpose

    Bacterial NM repression YFP reporter: protospacer A

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    NM prototspacer A/PAM with reporter EYFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    lambda pR promoter

    Available since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. SK-YFP-TD-A

    Plasmid

    #48666

    Purpose

    Bacterial TD repression YFP reporter: protospacer A

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    TD prototspacer A/PAM with reporter EYFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    lambda pR promoter

    Available since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pRC319

    Plasmid

    #61272

    Purpose

    Also referred to as pΦRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA targeting the NDM-1 gene. Contains a ColE1 origin, kanamycin resistance cassette and f1 origin for packaging in M13 particles.

    Depositor

    Timothy Lu

    Article

    Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103

    Insert

    Cas9, tracrRNA, crRNA
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. peft-3::cas-9::tbb-2 3'UTR

    Plasmid

    #48960

    Purpose

    this plasmid contains the codon optimized gene encoding Cas9 for C. elegans.

    Depositor

    Mario de Bono

    Article

    Chen et al Nucleic Acids Res. 2013 Sep 5.

    Insert

    cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    eef-1A.1 (eft-3)

    Available since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCfB8792

    Plasmid

    #126910

    Purpose

    Template for PCR amplification of ADE2 gRNA biobrick for targeting Saccharomyces cerevisiae ADE2 gene

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    ADE2 gRNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pWR95(URA3)

    Plasmid

    #203917

    Purpose

    BLINCAR plasmid for gene deletion, URA3 target

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    URA3 upstream homology
    URA3 downstream homology
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pWR95

    Plasmid

    #203916

    Purpose

    BLINCAR plasmid for gene deletion, no genomic target

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Insert

    modified MCS
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCF738

    Plasmid

    #122032

    Purpose

    EF1a-WNV-protease-GFP

    Depositor

    David Savage

    Article

    Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

    Inserts

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pU6a:sgRNA#1

    Plasmid

    #64245

    Purpose

    expresses sgRNA under U6a promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pU6b:sgRNA#3

    Plasmid

    #64247

    Purpose

    expresses sgRNA under U6b promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pU6c:sgRNA#4

    Plasmid

    #64248

    Purpose

    expresses sgRNA under U6c promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pU6a:sgRNA#2

    Plasmid

    #64246

    Purpose

    expresses sgRNA under U6a promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge