We narrowed to 32,159 results for: uros

1. pLV-CDH5-mRuby3-IRES-puro

   Plasmid

   #85144

   Purpose

   lentiviral expression of human VE-cadherin, C-terminally tagged with mRuby3

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Insert

   CDH5-mRuby3 (VE-cadherin, C-terminally tagged with mRuby3 (CDH5 Human)
   

   Use

   Lentiviral

   Tags

   mRuby3

   Promoter

   CAG

   Available Since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-DmrB-Casp8-2A-mCherry-puro

   Plasmid

   #231971

   Purpose

   Tet inducible expression of DmrB-Casp8 with mCherry to induce Casp8 apoptosis

   Depositor

   Brian Ruffell

   Article

   Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

   Insert

   Casp8 dimerizing construct with bi-cistronic mCherry (CASP8 Human)
   

   Use

   Lentiviral

   Available Since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCW-HA-hRb-delta-CDK-puro

   Plasmid

   #212673

   Purpose

   Express tagged hRb delta-CDK phosphosite mutant

   Depositor

   Yumi Konagaya, Tobias Meyer

   Article

   Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

   Insert

   Rb (RB1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   All 15 CDK phospho-sites are mutated to alanines

   Promoter

   TRE

   Available Since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSCV(gfp)U6sgRNA5(BbsI)-PGKpuroBFP

   Plasmid

   #102798

   Purpose

   Retrovirus for delivery of one sgRNA (self-targeting) - GFP-targeting control plasmid contains GFP instead of PuroR

   Depositor

   Sarah Teichmann

   Article

   Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

   Inserts

   sgRNA
   EGFP
   

   Use

   CRISPR and Retroviral

   Expression

   Mammalian

   Mutation

   H231L

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti CMV/TO RasV12 Puro (w119-1)

   Plasmid

   #22262

   Depositor

   Eric Campeau

   Insert

   HRAS G12V (HRAS Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   G12V mutant

   Available Since

   Dec. 15, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCEP-Puro-TetOn-L1RP-ORF1_mNeonGreen2

   Plasmid

   #202566

   Purpose

   Expresses an endogenous-like L1RP LINE-1 element with a C-terminal mNeonGreen2 fluorophore on the ORF1 protein for fluorescence microscopy

   Depositor

   Liam Holt

   Article

   Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

   Insert

   LINE-1
   

   Tags

   mNeonGreen2 on ORF1p

   Expression

   Mammalian

   Available Since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. N-Flag-IPO9_Lenti SFFV Puro WPRE

   Plasmid

   #217916

   Purpose

   Express IPO9 in mammalian cells

   Depositor

   Benjamin Cravatt

   Article

   Ogasawara et al Cell Chem Biol. 2024 Nov 7:S2451-9456(24

   Insert

   importin 9 (IPO9 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHDR_G3BP1-V5-APEX2-mScarlet-I EF1a-Puro

   Plasmid

   #196196

   Purpose

   homologous recombination donor top insert V5-APEX2-mScarlet-I into the C-terminus of the human G3BP1 locus

   Depositor

   Jürgen Knoblich

   Article

   Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

   Insert

   G3BP1 homology arms flanking V5-APEX2-mScarlet-I and EF1a-puro selection cassette (G3BP1 Human)
   

   Use

   Homology repair donor

   Tags

   V5-APEX2-mScarlet-I

   Available Since

   April 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-FLAG-puro-PTPIP51(1-470)

   Plasmid

   #170536

   Purpose

   Expresses PTPIP51(1-470) in human HeLa cells after transfection

   Depositor

   Byung Il Lee

   Article

   Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

   Insert

   Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
   

   Tags

   FLAG tag

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

10. pCMV6-G1-ATP6-Myc-FLAG (puromycin)

    Plasmid

    #86851

    Purpose

    mammalian expression of ATP6 with mitochondrial targeting sequence, Myc, and FLAG tags

    Depositor

    Matthew O'Connor

    Article

    Boominathan et al Nucleic Acids Res. 2016 Nov 2;44(19):934

    Insert

    ATP6 (ATP6 Human)
    

    Tags

    ATP5G1 (G1) (MQTAGALFISPALIRCCTRGLIRPVSASFLNS PVN…

    Expression

    Mammalian

    Mutation

    codon corrected

    Promoter

    CMV

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. AttB_ACE2-2A-TMPRSS2_IRES_iRFP670-H2A-P2A-PuroR

    Plasmid

    #190077

    Purpose

    express human ACE2 and human TMPRSS2 with selectable markers iRFP670 and puromycin resistance gene

    Depositor

    Kenneth Matreyek

    Article

    Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

    Insert

    ACE2 (ACE2, TMPRSS2 Human)
    

    Expression

    Mammalian

    Promoter

    none

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pL-U6-sgRNA-SFFV-Puro-P2A-EGFP

    Plasmid

    #175037

    Purpose

    Lentiviral delivery of sgRNA. Expresses PuroR and eGFP from SFFV promoter.

    Depositor

    Kristian Helin

    Article

    Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

    Insert

    Sp sgRNA scaffold
    

    Use

    Lentiviral

    Promoter

    U6

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti puro EF2 LUC-2A-Dendra2

    Plasmid

    #129427

    Purpose

    expression of LUC and Dendra2 reporters under EF2 promoter

    Depositor

    Oskar Laur

    Insert

    LUC Dendra2
    

    Use

    Lentiviral

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRISPaint-APEX2-GFP-3xFLAG-PuroR

    Plasmid

    #221238

    Purpose

    CRISPaint gene tagging

    Depositor

    Jonathan Kagan

    Article

    Fisch et al Nature. 2024 Jul;631(8021):635-644. doi:

    Insert

    APEX2-GFP-3xFLAG
    

    Use

    CRISPR

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. PiggyBac EF1a-eGFP-Puro U6 PPP2CA g2

    Plasmid

    #170823

    Purpose

    PiggyBac Cas13d sgRNA plasmid for PPP2CA knockdown

    Depositor

    Xiaoping Bao

    Article

    Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

    Insert

    Cas13d PPP2CA gRNA2
    

    Use

    Piggybac transposon

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLQ5465_pHR_hU6-crScaffold_EF1a-PuroR-T2A-BFP

    Plasmid

    #155307

    Purpose

    Rfx Cas13d guide cloning lentiviral vector with constitutively expressed puromycin resistance 2A-tagged with tagBFP

    Depositor

    Stanley Qi

    Article

    Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

    Insert

    Rfx Cas13d CRISPR RNA puromycin resistance 2A-tagged BFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hU6, EF1a

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCXLE-hOCT3/4-shp53-F + mCherry-2A-puro

    Plasmid

    #74947

    Purpose

    Dosage control and tracing of reprogramming episomes

    Depositor

    Ann Zovein

    Article

    Schmitt et al Stem Cell Res Ther. 2017 Jun 5;8(1):132.

    Insert

    Oct3/4 (POU5F1 Human)
    

    Expression

    Mammalian

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMJA284= pSin-Pur-MCS-Puro

    Plasmid

    #116877

    Purpose

    Lentiviral transfer vector containing a multible cloning site

    Depositor

    Marcos Alcocer

    Article

    Wang et al Biotechnol Lett. 2019 Oct;41(10):1095-11

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-MPAct-mRuby3-IRES-Puro

    Plasmid

    #155231

    Purpose

    lentiviral MPAct construct

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    Lentiviral expression of MPAct-mRuby sensor
    

    Use

    Lentiviral

    Tags

    mRuby3

    Available Since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDH-CB-IRES-copGFP-T2A-Puro

    Plasmid

    #72299

    Purpose

    Express gene of interest under CB promoter

    Depositor

    Kazuhiro Oka

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    chicken beta-actin + CMV enhancer

    Available Since

    Jan. 25, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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