We narrowed to 37,577 results for: ELL

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pSimpleII-NLS-NmCas9-HA-NLS(s)

Plasmid

#47867

Purpose

This plasmid encode NmCas9 with two NLS and a HA tag

Depositor

Erik Sontheimer, James Thomson

Publication

Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

Insert

NmCas9

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pGL2- Empty control -SV40-Luc

Plasmid

#26280

Purpose

Addgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.

Depositor

Rudolf Jaenisch

Publication

Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

Type

Empty backbone

Use

Luciferase

Expression

Mammalian

Available since

Oct. 20, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pAL101

Plasmid

#22474

Purpose

Expresses APB domain of PIF6 fused to mYFP, for red-light-induced binding to Phy

Depositor

Christopher Voigt

Publication

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

PIF6APB

Use

Synthetic Biology

Tags

mYFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 25, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-EWB-DIO-cyan pre-eGRASP(p32)

Plasmid

#111589

Purpose

An AAV vector that expresses double floxed cyan pre-eGRASP(p32) under the Ef1a promoter.

Depositor

Bong-Kiun Kaang

Publication

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

cyan pre-eGRASP(p32)

Use

AAV

Promoter

Ef1a

Available since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA5-FRT-TO-NOD2-EGFP

Plasmid

#131207

Purpose

mammalian expression, tet R

Depositor

Thomas Kufer

Publication

Pei et al EMBO J. 2021 Jul 1;40(13):e106272. doi:

Insert

NOD2 (NOD2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

pCMV

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pBp-FGFR2c-WT

Plasmid

#45699

Depositor

Matthew Meyerson

Publication

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIc (FGFR2 Human)

Use

Retroviral

Expression

Mammalian

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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syntaxin3-myc-myc-His

Plasmid

#12372

Depositor

Thomas Weimbs

Publication

Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

Insert

Syntaxin3 (STX3 Human)

Tags

2xMyc-His

Expression

Mammalian

Mutation

Insert is cloned into a modified vector

Available since

Sept. 28, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pClneoMyc-LATS1

Plasmid

#66851

Purpose

Express Myc-tagged LATS1 in mammalian cells

Depositor

Yutaka Hata

Publication

Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

Insert

LATS1 (LATS1 Human)

Tags

Myc

Expression

Mammalian

Mutation

P44L mutation in LATS1 compared to GenBank refere…

Promoter

CMV

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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WT lamin A minigene

Plasmid

#20291

Depositor

Tom Misteli

Publication

Scaffidi et al Nat Med. 2005 Apr . 11(4):440-5.

Insert

lamin A (LMNA Human)

Tags

GFP

Expression

Mammalian

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pC1-HyPerRed-mito

Plasmid

#60247

Purpose

Mitochondria-targeted genetically encoded hydrogen peroxide indicator with red fluorescence.

Depositor

Vsevolod Belousov

Publication

Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

Insert

HyPerRed-mito

Tags

Mitochondrial targeting signal

Expression

Mammalian

Promoter

CMV

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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sgCtr- LentiCRISPRv2

Plasmid

#107402

Purpose

Lentiviral expression of Cas9 and a control gRNA

Depositor

William Kaelin

Publication

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

control gRNA

Use

Lentiviral

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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p323-L10A-PATagRFP

Plasmid

#74172

Purpose

Fluorescent reporter for single ribosome tracking

Depositor

Robert Singer

Publication

Katz et al Elife. 2016 Jan 13;5. pii: e10415. doi:

Insert

ribosomal protein L10A (Rpl10a Rat)

Use

Lentiviral

Tags

PATagRFP

Promoter

UbC

Available since

April 4, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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HA-Mob

Plasmid

#32835

Depositor

Kunliang Guan

Publication

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

Mob (MOB1A Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Nov. 15, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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Str-KDEL_neomycin

Plasmid

#65306

Purpose

Luminal ER hook only (RUSH system)

Depositor

Franck Perez

Publication

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end

Tags

KDEL motif and Signal Sequence

Expression

Mammalian

Promoter

CMV

Available since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova)

Plasmid

#85008

Purpose

For Flpe/FRT-based Supernova-GFP, pK068 should be used with pK036. The GFP fragment of pK068 can be replaced with another gene by SalI and EcoRV.

Depositor

Takuji Iwasato

Publication

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

EGFP-ires-tTA

Expression

Mammalian

Available since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (AAV9)

Viral prep

#55645-AAV9

Purpose

Ready-to-use AAV9 particles produced from pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (#55645). In addition to the viral particles, you will also receive purified pAAV-hSyn Con/Fon hChR2(H134R)-EYFP plasmid DNA. Synapsin-driven, Cre-dependent and Flp-dependent channelrhodopsin-EYFP expression for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

Depositor

Karl Deisseroth

Publication

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Promoter

Syn

Tags

EYFP (Cre- and Flp-dependent)

Available since

June 8, 2020

Availability

Academic Institutions and Nonprofits only
AAVS1_Puro_hPGK1_EGFP_Donor

Plasmid

#178088

Purpose

HDR donor plasmid to introduce the EGFP cassette to AAVS1 using AAVS1 T2 sgRNA.

Depositor

Yannick Doyon

Publication

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

EGFP

Use

CRISPR

Expression

Mammalian

Promoter

Human PGK1

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pPS

Plasmid

#85801

Purpose

Backbone for pooled cloning of Perturb-seq libraries

Depositor

Aviv Regev

Publication

Dixit et al Cell. 2016 Dec 15;167(7):1853-1866.e17.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

March 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pQE30-His-PheRS

Plasmid

#124116

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Phenylalanyl-tRNA ligase (pheS, pheT Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLKO.1-GSK3β-#2

Plasmid

#32497

Depositor

Alex Toker

Publication

Yoeli-Lerner et al Mol Cancer Res. 2009 Mar;7(3):425-32. Ep

Insert

GSK3B (GSK3B Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available since

Sept. 26, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles