We narrowed to 30,802 results for: REP

1. pX330A_D10A-1x5

   Plasmid

   #58775

   Purpose

   Expresses Cas9 nickase and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Promoter

   CBh

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBFv-UAS3

   Plasmid

   #138399

   Purpose

   10xUAS expression vector with a vermilion marker and a phiC31 attB sequence

   Depositor

   Shu Kondo

   Article

   Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   UAS

   Available since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. PNCA-GFP-dYY1

   Plasmid

   #67791

   Purpose

   Use together with pCMV-intron and VSV-G to package MMLV reporter virus

   Depositor

   Stephen Goff

   Article

   Schlesinger et al Cell Rep. 2013 Jul 11;4(1):50-8. doi: 10

   Insert

   MMLV-LTR-GFP
   

   Use

   Tags

   Expression

   Mutation

   Deletion of LTR U3 YY1 binding domain

   Promoter

   Available since

   Sept. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pKO (Kan)

   Plasmid

   #110086

   Purpose

   Suicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Use

   Bacterial knockout generation

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPB-EF1a-MegaGate-HA-Hygro

   Plasmid

   #175268

   Purpose

   MegaGate destination with C-terminal HA tag and hygromycin resistance

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEF6.HA-ARRDC3

   Plasmid

   #38317

   Depositor

   Quan Lu

   Article

   Nabhan et al EMBO Rep. 2010 Aug;11(8):605-11. Epub 20

   Insert

   ARRDC3 (ARRDC3 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. MAC_C_LMTK3

   Plasmid

   #187773

   Purpose

   MAC-tagged gene expression of human LMTK3

   Depositor

   Markku Varjosalo

   Article

   Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

   Insert

   LMTK3 (LMTK3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-EF1a-MegaGate-HA-Blast

   Plasmid

   #175270

   Purpose

   MegaGate destination with C-terminal HA tag and blasticidin resistance

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. TOPO GFP

   Plasmid

   #68488

   Purpose

   TOPO construct expressing GFP for generation of GMAP-compatible gene

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   Green Fluorescent Protein
   

   Use

   Gmap

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Plac

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCZGY2850

    Plasmid

    #87758

    Purpose

    Pcol-19-PH::miniSOG(Q103L), expression of enhanced mutant version of membrane targeted PH-miniSOG (Q103L) in epidermis.

    Depositor

    Andrew Chisholm

    Article

    Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

    Insert

    Pcol-19-PH-miniSOG(Q103L)
    

    Use

    Tags

    PH

    Expression

    Worm

    Mutation

    Q103L

    Promoter

    Pcol-19

    Available since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. Parp14-c011

    Plasmid

    #173098

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    PARP14 (PARP14 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    K791-F978

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. A1-20B1(P004)

    Plasmid

    #184056

    Purpose

    This plasmid carries Yn situ preamplifier A1-20B1 sequence and is used for nickase based synthesis.

    Depositor

    C. Ron Yu

    Article

    Wu et al Cell Rep Methods. 2022 Apr 18;2(4):10020

    Insert

    A1-20B1 preamplifier
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. mito-mGarnet

    Plasmid

    #104309

    Purpose

    codes for a fusion of fluorescent protein mGarnet and mito, stains mictochondrial content in live cells, i.e. human cytochrome c oxidase subunit VIII, the mitochondrial targeting sequence mito

    Depositor

    Ulrich Nienhaus

    Article

    Hense et al Sci Rep. 2015 Dec 9;5:18006. doi: 10.103

    Insert

    mito-mGarnet
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKO (Apr)

    Plasmid

    #110088

    Purpose

    Suicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Use

    Bacterial knockout generation

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMK486 (Hygro-P2A-2mAID)

    Plasmid

    #214398

    Purpose

    2mAID for C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    Hygro-P2A-2mAID
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-DUET-1-mRhubarb713-HO

    Plasmid

    #141199

    Purpose

    Expresses mRhubarb713 fluorescent protein and heme oxygenase

    Depositor

    Elad Firnberg

    Article

    Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

    Inserts

    mRhubarb713
    heme oxygenase
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    R134H, W309R, Q310A compared to iRFP

    Promoter

    T7

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. eGFP L202 gRNA

    Plasmid

    #119132

    Purpose

    gRNA that guides Cas9 and APOBEC complexes to L202 in eGFP.

    Depositor

    Reuben Harris

    Article

    Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

    Insert

    eGFP L202 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    None

    Promoter

    U6

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMK464 (mAB-Neo)

    Plasmid

    #214390

    Purpose

    mAID-BromoTag for C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    mAID-BromoTag-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. cmv-sfGFP-GB1-scAB-ubc-nls-pcp-12xSunTag(SRv1.2-2P)

    Plasmid

    #185802

    Purpose

    one plasmid encoding scFv GFP and pcp-10xSunTag components for SunRISER SRv1.2

    Depositor

    Robin Lee

    Article

    Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

    Insert

    cmv-sfGFP-GB1-scAB--ubc-nls-pcp-12xSunTag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFA6a-link-ymYPET-URA3

    Plasmid

    #168056

    Purpose

    Replaces Addgene plasmid 118460. Yeast tagging vector to create a gene fusion with yeast optimized mYPET with CaUra3 selection; Contains the linker region at the 5’ end of the FP

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymYPET
    

    Use

    Tags

    ymYPET

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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