We narrowed to 55,915 results for: plasmid

1. pWPI-IRES-Puro-Ak-ACE2

   Plasmid

   #154985

   Purpose

   Lentiviral vector expressing Human ACE2

   Depositor

   Sonja Best

   Insert

   ACE2 (ACE2 Human)
   

   Use

   Lentiviral

   Available Since

   July 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-Flex-Axon-EGFP

   Plasmid

   #135429

   Purpose

   Can be used to drive axon-enriched expression of EGFP in the presence of Cre recombinase.

   Depositor

   Rylan Larsen

   Has Service

   AAV1

   Insert

   axon-EGFP
   

   Use

   AAV and Cre/Lox

   Tags

   GAP43 palmitoylation domain

   Expression

   Mammalian

   Promoter

   hSyn1

   Available Since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-BE4max-P2A-EGFP (pRZ33)

   Plasmid

   #140083

   Purpose

   CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with P2A-EGFP

   Depositor

   Keith Joung

   Insert

   Human codon optimized pCAG BE4max with P2A-EGFP
   

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   D10A (SpCas9)

   Promoter

   CAG

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR221 LRRK2 wt

   Plasmid

   #39529

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Gateway compatible cloning vector

   Available Since

   Oct. 1, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. Q-IL6-9

   Plasmid

   #166552

   Purpose

   scFv of a human scaffold targeting Interleukin-6. Antigen coverage aa 28-212 of 212, full-length excl. signal peptide

   Depositor

   Susanne Gräslund

   Insert

   single chain-Fv, scFv
   

   Use

   Recombinant antibody expression

   Tags

   3xFLAG, Sortag, His6 and OmpA leader sequence

   Expression

   Bacterial

   Promoter

   LacZ

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOttc1501 - pscAAV CMV-IE hCDNF

   Plasmid

   #188538

   Purpose

   An AAV packaging vector that expresses hCDNF under control of the CMV-IE promoter.

   Depositor

   Brandon Harvey

   Insert

   hCDNF (CDNF Human)
   

   Use

   AAV

   Promoter

   CMV-IE

   Available Since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. P48

   Plasmid

   #212155

   Purpose

   Plasmid expressing 6x His tagged secondary alcohol dehydrogenase fused with superfolder green fluorescent protein at N terminus under constitutive promoter PgyrA control.

   Depositor

   Kang Zhou

   Insert

   superfolder green fluorescent protein, secondary alcohol dehydrogenase
   

   Tags

   6x His

   Expression

   Bacterial

   Promoter

   PgyrA

   Available Since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWPI-IRES-Bla-Ak-ACE2

   Plasmid

   #154981

   Purpose

   Lentiviral vector expressing Human ACE2

   Depositor

   Sonja Best

   Insert

   ACE2 (ACE2 Human)
   

   Use

   Lentiviral

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX6P1 METTL3

   Plasmid

   #200731

   Purpose

   Bacterial expression of GST-Tagged codon optimized human METTL3

   Depositor

   Claudio Alarcón

   Insert

   METTL3 (METTL3 Human)
   

   Tags

   GST from backbone plasmid

   Expression

   Bacterial

   Mutation

   Codon optimized for expression in E. coli

   Available Since

   July 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. IMBB2.4-pUC57-mini

    Plasmid

    #88845

    Purpose

    plasmid for "Tip Snip" cloning (described in forthcoming Biotechniques article)

    Depositor

    Ichiro Matsumura

    Article

    Matsumura et al Biotechniques. 2017 Mar 1;62(3):99-106.

    Type

    Empty backbone

    Use

    Synthetic Biology; Cloning

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. SIRT6

    Plasmid

    #41564

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SIRT6 (SIRT6 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    aa 3-318

    Promoter

    T7

    Available Since

    April 9, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

12. T-IL2-12

    Plasmid

    #166564

    Purpose

    scFv of a human scaffold targeting Interleukin-2. Antigen coverage aa 21-153 of 153, full-length excl. signal peptide

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, Sortag, His6 and OmpA leader sequence

    Expression

    Bacterial

    Promoter

    LacZ

    Available Since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Q-IL18-6

    Plasmid

    #166554

    Purpose

    scFv of a human scaffold targeting Interleukin-18. Antigen coverage aa 37-193 of 193, full-length excl. signal peptide

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, Sortag, His6 and OmpA leader sequence

    Expression

    Bacterial

    Promoter

    LacZ

    Available Since

    April 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. AiP13318 - pAAV-AiE0390m_3xC2-minBG-SYFP2-4x2C-WPRE3-BGHpA (Alias: CN3318)

    Plasmid

    #208167

    Purpose

    AiE0390m_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated transgene expression in brain astrocytes

    Depositor

    Allen Institute for Brain Science, Jonathan Ting

    Insert

    SYFP2
    

    Use

    AAV

    Mutation

    NA

    Promoter

    Beta Globin minimal promoter

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.2-V5-DEST-hSFRP1-VF

    Plasmid

    #189542

    Purpose

    Protein expression of SFRP1 in mammalian cells.

    Depositor

    Haian Fu

    Insert

    secreted frizzled related protein 1 (SFRP1 Human)
    

    Tags

    VF

    Expression

    Mammalian

    Available Since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEMB52 / Human_LRRK1_1_360

    Plasmid

    #40549

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    LRRK1 (LRRK1 Human)
    

    Use

    Baculovirus

    Tags

    6xHis

    Mutation

    WT

    Promoter

    polH

    Available Since

    Oct. 1, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pAAV hSyn-Flex RG-cerulean

    Plasmid

    #98221

    Purpose

    Cre dependent Cerulean-E2A-SADB19 rabies glycoprotein expression, under hSyn promoter, for producing AAV virus

    Depositor

    Troy Margrie

    Insert

    Cerulean-E2A-RG
    

    Use

    AAV

    Tags

    cerulean

    Promoter

    human Synapsin

    Available Since

    Aug. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. SDCBPA-c000

    Plasmid

    #175480

    Purpose

    Protein expression in bacterial cells. Entire CDS, M1-V288.

    Depositor

    Opher Gileadi

    Insert

    SDCBP (SDCBP Human)
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUPD2 PhyB (GB2721)

    Plasmid

    #160603

    Purpose

    chimeric construct comprising the N-terminal fragment of A. thaliana PhyB (amino acids 1-650) fused to the VP16 activation domain and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switch.

    Depositor

    Diego Orzaez

    Insert

    PhyB
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. Z-AIMP1-1

    Plasmid

    #166560

    Purpose

    scFv of a human scaffold targeting Aminoacyl tRNA synthase complex-interacting multifunctional protein 1. Antigen coverage aa 147-312 of 312

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Promoter

    LacZ

    Available Since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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