We narrowed to 116,650 results for: mpi

1. 1553 pcDNA3 HA gamma adaptin 2

   Plasmid

   #10713

   Depositor

   William Sellers

   Insert

   Gamma adaptin 2 (AP1G2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2005

   Availability

   Academic Institutions and Nonprofits only

2. AAV-syn-axon-jGCaMP8m-WPRE

   Plasmid

   #186042

   Purpose

   jGCaMP8m expression in axons under the control of the synapsin promoter

   Depositor

   Loren Looger

   Insert

   axon-jGCaMP8m
   

   Use

   AAV

   Tags

   GAP43-6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   synapsin

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRSII405

   Plasmid

   #35440

   Depositor

   Steven Haase

   Article

   Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

   Type

   Empty backbone

   Use

   Yeast integrating vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSLAX13 (CT#232)

   Plasmid

   #13918

   Depositor

   Cliff Tabin

   Article

   Logan et al Methods. 1998 Apr . 14(4):407-20.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 9, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pnos-Fok1:dCas9-nos

   Plasmid

   #62210

   Purpose

   Expresses Fok1:dCas9 under control of nanos promoter and 3'UTR. For germ line restricted high specificity genome engineering in Drosophila melanogaster.

   Depositor

   Simon Bullock

   Insert

   Fok1-dCas9
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   nanos

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SPOCK2_22-424_AviN_HisC

   Plasmid

   #195877

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   SPOCK2 (SPOCK2 Human)
   

   Use

   Pfhmsp-avin-lic-c, baculovirus expression

   Tags

   Expression

   Mutation

   Promoter

   Polyhedrin

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRSII306

   Plasmid

   #35441

   Depositor

   Steven Haase

   Article

   Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

   Type

   Empty backbone

   Use

   Yeast integrating vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET15b/GgVcl 884-1066-ECFP

   Plasmid

   #46182

   Depositor

   Susan Craig

   Insert

   Vcl 884-1066 (VCL Chicken)
   

   Use

   Tags

   ECFP and His

   Expression

   Bacterial

   Mutation

   aa 884-1066 (tail domain)

   Promoter

   T7

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pGRB2.1

   Plasmid

   #45341

   Depositor

   Brendan Cormack

   Article

   Zordan et al G3 (Bethesda). 2013 Oct 3;3(10):1675-86.

   Type

   Empty backbone

   Use

   C. glabrata

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TRIOBP-TwinStrepII

    Plasmid

    #83201

    Purpose

    Expression of tagged protein in mammalian cells

    Depositor

    Peter Barr-Gillespie

    Insert

    TRIOBP (Triobp Mouse)
    

    Use

    Tags

    Twin Strep Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLV-NT-3-mCherry

    Plasmid

    #190201

    Purpose

    To express the mouse NT-3 tagged with the FP mCherry

    Depositor

    Pantelis Tsoulfas

    Insert

    NT-3
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    The codon were optimized for better expression

    Promoter

    CMV

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKB233

    Plasmid

    #170040

    Purpose

    Encodes PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Insert

    mNeonGreen
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PvirK

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTH385-GST-TEV-SUP35

    Plasmid

    #29744

    Depositor

    Tobias von der Haar

    Insert

    SUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    wild type

    Promoter

    Available since

    Aug. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. IDG_PANX2_OE_1

    Plasmid

    #161697

    Purpose

    Overexpresses PANX2

    Depositor

    Mike McManus

    Insert

    PANX2 (PANX2 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGL3-Basic-OPN-1200

    Plasmid

    #11996

    Depositor

    Steven Johnson

    Insert

    Osteopontin (SPP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The following construct contains the OPN promoter…

    Promoter

    Available since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pLIX403-EBFP2-ccdB-G418

    Plasmid

    #158556

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAc5.1B-EGFP-DmRRP40

    Plasmid

    #65797

    Purpose

    expresses EGFP-tagged RRP40 in Drosophila cells

    Depositor

    Elisa Izaurralde

    Insert

    RRP40 (Rrp40 Fly)
    

    Use

    Drosophila expression

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Actin 5C

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPro24

    Plasmid

    #17805

    Depositor

    Jay Keasling

    Article

    Lee et al Appl Environ Microbiol. 2005 Nov . 71(11

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 28, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pLenti-ePEG-LINE1-loxPsym

    Plasmid

    #216866

    Purpose

    Lentiviral guide RNA vector to insert the loxPsym sequence into LINE1 elements

    Depositor

    Leopold Parts

    Insert

    spacerLINE1_cr772scaffold_PBS-loxPsm-HA-tevopreQ
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MIG-REST

    Plasmid

    #91898

    Purpose

    Expression of mouse REST

    Depositor

    Julien Sage

    Insert

    REST (Rest Mouse)
    

    Use

    Retroviral

    Tags

    ires-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. IDG_TMC5_OE_1

    Plasmid

    #161621

    Purpose

    Overexpresses TMC5

    Depositor

    Mike McManus

    Insert

    TMC5 (TMC5 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET MBP TEV expression vector with BrioBrick polypromoter restriction sites (14-Q)

    Plasmid

    #48311

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. mouse ptc probe M2-3

    Plasmid

    #58701

    Purpose

    makes mouse ptc probe for in situ hybridization

    Depositor

    Matthew Scott

    Insert

    ptc (Ptch1 Mouse)
    

    Use

    In situ probe

    Tags

    Expression

    Bacterial

    Mutation

    only EcoRI fragment (5' end)

    Promoter

    T7

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

24. pJF3H

    Plasmid

    #63897

    Purpose

    For phage display of cDNA libraries

    Depositor

    Carlos Barbas

    Article

    Barbas et al Cold Spring Harbor, NY: Cold Spring Harb

    Insert

    cDNA cloning region
    

    Use

    Phage display

    Tags

    Fos

    Expression

    Bacterial

    Mutation

    Promoter

    lacZ

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pKSB- 3xP3-mTurqSV40 GGGG AAGA

    Plasmid

    #174533

    Purpose

    3xP3-mTurquoise fluorescence marker cassette for gene knock-in in mosquitoes

    Depositor

    Eric Marois

    Insert

    3xP3-mTurquoise BsaI cassette
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    3xP3

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pB-CAG-PE2-NatMX

    Plasmid

    #158567

    Purpose

    Piggybac vector that allows for the generation of PE2-expressing stable cell lines

    Depositor

    Alejandro Chavez

    Insert

    PE2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. IDG_TMC4_OE_1

    Plasmid

    #161652

    Purpose

    Overexpresses TMC4

    Depositor

    Mike McManus

    Insert

    TMC4 (TMC4 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLIX403-EBFP2-ccdB-Blast

    Plasmid

    #158557

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJK615

    Plasmid

    #71699

    Purpose

    Produces E. coli thioredoxin with a C-terminal His6 tag (EcTrxAH6)

    Depositor

    T. J. Kappock

    Insert

    thioredoxin
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLXIN2-RFP

    Plasmid

    #99205

    Purpose

    RFP expressing bicistronic retroviral vector

    Depositor

    Saroj Mathupala

    Insert

    Red fluorescent protein
    

    Use

    Retroviral; Bicistronic retroviral expression vec…

    Tags

    Expression

    Mammalian

    Mutation

    The dsRed cDNA was PCR amplified off AddGene plas…

    Promoter

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pPD708 DsRed-Express2-ZF21

    Plasmid

    #138795

    Purpose

    Constitutive expression of DsRed-Express2-ZF21 under the CMV promoter.

    Depositor

    Joshua Leonard

    Insert

    DsRed-Express2-ZF21
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. WT-long-UBASH3A-V5-6xHis

    Plasmid

    #192101

    Purpose

    Mammalian expression of human wild-type long-form UBASH3A with V5-6xHis tag

    Depositor

    Patrick Concannon

    Insert

    UBASH3A (UBASH3A Human)
    

    Use

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pUA97

    Plasmid

    #69340

    Purpose

    It encodes a fluorescent protein that can be fused to a gene of interest using SEC CRISPR strategy

    Depositor

    Oliver Hobert

    Insert

    ceBFP SEC 3xFlag
    

    Use

    Tags

    3xFlag and ceBFP (mTagBFP)

    Expression

    Worm

    Mutation

    sqt-1(e1350)

    Promoter

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. AAV-syn-LifeAct-jGCaMP8m-WPRE

    Plasmid

    #186039

    Purpose

    AAV-mediated expression of LifeAct-jGCaMP8m under the synapsin promoter

    Depositor

    Loren Looger

    Insert

    LifeAct-jGCaMP8m
    

    Use

    AAV

    Tags

    LifeAct-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. AAV-syn-axon-jGCaMP8f-WPRE

    Plasmid

    #186041

    Purpose

    jGCaMP8f expression in axons under the control of the synapsin promoter

    Depositor

    Loren Looger

    Insert

    axon-jGCaMP8f
    

    Use

    AAV

    Tags

    GAP43-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRL-TK CXCR4 6x

    Plasmid

    #11308

    Depositor

    Phil Sharp

    Article

    Doench et al Genes Dev. 2003 Feb 15. 17(4):438-42.

    Insert

    6 bulged bind sites for CXCR4 siRNA antisense
    

    Use

    Luciferase

    Tags

    Rr-luc

    Expression

    Mutation

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pExp-PpiB

    Plasmid

    #112572

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and PpiB (peptidyl-prolyl cis-trans isomerase B from E. coli) fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis-PpiB and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHH0103 NEDD4 WW domain #3

    Plasmid

    #104210

    Purpose

    Bacterial expression of WW domain #3 from NEDD4

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    NEDD4 WW domain #3 (NEDD4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBMTBX-1

    Plasmid

    #26072

    Depositor

    Ryan Gill

    Article

    Prior et al Biotechnol Bioeng. 2010 Jun 1. 106(2):32

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pHH0103 NEDD4L WW domain #2

    Plasmid

    #104205

    Purpose

    Bacterial expression of WW domain #2 from NEDD4L

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    NEDD4L WW domain #2 (NEDD4L Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. T7-U4

    Plasmid

    #111336

    Purpose

    for in vitro snRNA transcription

    Depositor

    Christine Guthrie

    Article

    Ghetti et al RNA. 1995 Apr;1(2):132-45.

    Insert

    snR14
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pAc5.1B-EGFP-DmG3BP_G

    Plasmid

    #146349

    Purpose

    Insect Expression of DmG3BP

    Depositor

    Elisa Izaurralde

    Insert

    DmG3BP (rin Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    V632G compared to AF231031.1, exact match to NP_5…

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pINER5

    Plasmid

    #185895

    Purpose

    In vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).

    Depositor

    Claudia Vickers

    Insert

    PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PSk.GAL2>YFAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 25, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

44. LHP398 - Ub-K48R/K63R

    Plasmid

    #32167

    Depositor

    Linda Hicke

    Insert

    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    K48R and K63R

    Promoter

    CUP1

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

45. pGAL-flp(H305L)-NAT

    Plasmid

    #160380

    Purpose

    overexpression of Flp recombinase step-arrest mutant in yeast

    Depositor

    John McCusker

    Insert

    FLP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    H305L

    Promoter

    GAL

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pGEX-MK2

    Plasmid

    #30187

    Depositor

    Bruce Tempel

    Insert

    MK2 (Kcna2 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

47. pLinker-BirA-3xHA-HXGPRT-loxP

    Plasmid

    #86668

    Purpose

    for generation of BirA tagged strains using CRISPR tagging technology

    Depositor

    David Sibley

    Insert

    Linker-BirA-3xHA-HXGPRT 3'UTR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pZHY051

    Plasmid

    #44987

    Depositor

    Daniel Voytas

    Article

    Zhang et al Plant Physiol. 2012 Nov 2.

    Type

    Empty backbone

    Use

    Plant expression

    Tags

    Expression

    Mutation

    Promoter

    35S

    Available since

    June 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pHH0103 NEDD4 WW domain #1

    Plasmid

    #104208

    Purpose

    Bacterial expression of WW domain #1 from NEDD4

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    NEDD4 WW domain #1 (NEDD4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Feb. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pTYB2-eIF1

    Plasmid

    #37217

    Depositor

    Jon Lorsch

    Article

    Acker et al Methods Enzymol. 2007;430:111-45.

    Insert

    eIF1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only