Addgene

We narrowed to 25,734 results for: STI;

Showing: 241 - 260 of 25734 results

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pIVTR(T7A)-ATOH1 (SA Substitutions)

Plasmid

#172309

Purpose

In vitro transcription of ATOH1 (Ser phosphosites substituted by Ala)

Depositor

Patrik Verstreken

Insert

ATOH1 (ATOH1 Human)

Use

Other

Tags

Expression

Mutation

S331A, S342A

Promoter

T7 promoter A-initiating

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT/TO MycLAP-STIL 5A

Plasmid

#80267

Purpose

mammalian expression plasmid for MycLAP-STIL 5A

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

STIL (STIL Human)

Use

Tags

Myc-GFP

Expression

Mammalian

Mutation

S1103A, S1108A, S1111A, S1116A, T1119A

Promoter

CMV

Available since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pLEX-FKBP-Kras-Blasticidin

Plasmid

#120714

Purpose

Expresses membrane-targeted recruiter protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

KRAS4B (KRAS Human)

Use

Lentiviral

Tags

FKBP and mCherry

Expression

Mammalian

Mutation

amino acids 158-188

Promoter

CMV

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pRW006-SM-destination-pro35S-Cas9

Plasmid

#104439

Purpose

Destination vector expressing plant-codon-optimized Cas9 under 35S promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free

Depositor

Detlef Weigel

Article

Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial and Plant

Mutation

Promoter

35S

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pIVTR(T7A)-NGN2 (SA Substitutions)

Plasmid

#172308

Purpose

In vitro transcription of NGN2 (Ser phosphosites substituted by Ala)

Depositor

Patrik Verstreken

Insert

NGN2

Use

Other

Tags

Expression

Mutation

S24A, S193A, S207A, S209A, S219A, S232A, S239A an…

Promoter

T7 promoter A-initiating

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-PP-Spastin(87-616)DeltaExon4

Plasmid

#128794

Purpose

Bacteiral expression of Spastin M87 varient without exon 4

Depositor

Chris Hill

Article

Han et al J Biol Chem. 2019 Nov 25. pii: AC119.009

Insert

Spastin (SPAST Human)

Use

`

Tags

GST

Expression

Bacterial

Mutation

residues 87-616 without exon 4.

Promoter

T7

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pEX-CFP-STIM1-deltaK

Plasmid

#18860

Depositor

Tobias Meyer

Article

Liou et al Proc Natl Acad Sci U S A. 2007 May 29. 1

Insert

STIM1 (STIM1 Human)

Use

Tags

CFP

Expression

Mammalian

Mutation

Stop codon at amino acid position 671 of STIM1. C…

Promoter

Available since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pLVX-mCherry-SPASTIN, F124D

Plasmid

#180648

Purpose

Lentiviral expression vector for SPASTIN. Has F124D mutation. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. SPASTIN starts on M87. Internal ID: WISP20-44.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPASTIN

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Starts at M87, F124D mutation, has siRNA resistan…

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLEX-FKBP-BclXL-Blasticidin

Plasmid

#120718

Purpose

Expresses mitochondrial membrane-targeted recruiter protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

Bl2-like protein 1, isoform Bcl-X(L)

Use

Lentiviral

Tags

FKBP and mCherry

Expression

Mammalian

Mutation

33 c-terminal amino acids

Promoter

CMV

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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Beta-arrestin2 K11,12R GFP

Plasmid

#35412

Depositor

Robert Lefkowitz

Article

Shenoy et al J Biol Chem. 2005 Apr 15;280(15):15315-2

Insert

βarrestin2 (Arrb2 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

K11,12R

Promoter

CMV

Available since

March 16, 2012

Availability

Academic Institutions and Nonprofits only
pecALPS gag-gfp Blasti

Plasmid

#115805

Purpose

Express gag-gfp fusion driven by EIF1a promoter

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

gag-gfp

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EIF1a

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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ptcALPS gag-gfp Blasti

Plasmid

#115806

Purpose

Express gag-gfp fusion driven by TK promoter

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

gag-gfp

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TK

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Collybistin [L120/12R]

Plasmid

#177575

Purpose

Mammalian Expression Plasmid of anti-Collybistin (Rat). Derived from hybridoma L120/12.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Collybistin (Rattus norvegicus) recombinant mouse monoclonal antibody (Arhgef9 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV syn Arrestin-TEVp-V5

Plasmid

#173118

Purpose

Expresses arrestin receiver construct in neurons

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

Arrestin-TEVp-V5

Use

AAV

Tags

Expression

Mutation

Promoter

Synapsin

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT/TO MycLAP-STIL ∆CC

Plasmid

#80268

Purpose

mammalian expression plasmid for MycLAP-STIL coiled-coil deletion

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

STIL (STIL Human)

Use

Tags

Myc-GFP

Expression

Mammalian

Mutation

missing conserved coiled-coil (amino acids 721-74…

Promoter

CMV

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-MC38-TCRA-30-picky_sticky

Plasmid

#138983

Purpose

IVT template for the alpha subunit of the mouse TCR #30 that is reactive against MC38

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRA (Tcra Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-MC38-TCRB-30-picky_sticky

Plasmid

#138984

Purpose

IVT template for the beta subunit of the mouse TCR #30 that is reactive against MC38

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRB (Tcrb Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-Collybistin [L120/30R]

Plasmid

#177465

Purpose

Mammalian Expression Plasmid of anti-Collybistin (Rat). Derived from hybridoma L120/30.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Collybistin (Rattus norvegicus) recombinant mouse monoclonal antibody (Arhgef9 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX-TCRb-FKBP-Blasticidin

Plasmid

#120723

Purpose

Expresses ER-targeted recruiter protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

T cell receptor β chain

Use

Lentiviral

Tags

FKBP and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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PX395 Roseburia intestinalis Cas9

Plasmid

#68332

Purpose

Roseburia intestinalis Cas9

Depositor

Feng Zhang

Insert

RiCas9

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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