We narrowed to 2,709 results for: dcas9

1. pGinB-8X(GGS)-dCas9-FLAG-NLS_SpecR

   Plasmid

   #81206

   Purpose

   CMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus. Spec resistance

   Depositor

   David Liu

   Article

   Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

   Insert

   pGinB-8X(GGS)-dCas9-FLAG-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZLCv2-gMYOD1-3xFLAG-dCas9-HA-2xNLS

   Plasmid

   #106355

   Purpose

   CRISPR/Cas9 engineered chromatin immunoprecipitation of MYOD1 locus

   Depositor

   Stephen Tapscott

   Article

   Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

   Insert

   MYOD1 gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZLCv2-gD4Z4-2-3xFLAG-dCas9-HA-2xNLS

   Plasmid

   #106353

   Purpose

   CRISPR/Cas9 engineered chromatin immunoprecipitation of D4Z4 locus

   Depositor

   Stephen Tapscott

   Article

   Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

   Insert

   D4Z4 gRNA-2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBDKRB2_TCS(Q’L)_HA-dCas9(N)_DHFR

   Plasmid

   #101109

   Purpose

   Encodes dCas9(N)-DHFR fused to BDKRB2

   Depositor

   Tudor Fulga

   Article

   Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

   Insert

   BDKRB2-dCas9(N)-DHFR
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA-dCas9-p300 Core (C1204R)

   Plasmid

   #61361

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation C1204R) driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9; C1204R mutation i…

   Promoter

   CMV

   Available since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-SFFV-KRAB-dCas9-P2A-mCherry

   Plasmid

   #60954

   Purpose

   2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9 fusion protein and mCherry

   Depositor

   Jonathan Weissman

   Article

   Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

   Insert

   KRAB-dCas9-P2A-mCherry fusion
   

   Use

   CRISPR and Lentiviral

   Tags

   2x NLS, HA, KRAB domain, and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Jan. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV hUbC-dCas9 KRAB-T2A-GFP

   Plasmid

   #67620

   Purpose

   Co-expressed human optimized S. pyogenese dCas9 fused to KRAB repressor domain and GFP

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Insert

   humanized dead Cas9 KRAB T2A GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mutation

   D10A and H840A

   Promoter

   Available since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV hUbC-dCas9-MQ1(Q147L)-EGFP

   Plasmid

   #89793

   Purpose

   Targeted CpG methylation

   Depositor

   Margaret Goodell

   Article

   Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

   Insert

   site-specific DNA-methyltransferase SssI
   

   Use

   CRISPR and Lentiviral

   Tags

   3*FLAG and T2A-EGFP

   Expression

   Mammalian

   Mutation

   Q147L, CAA-->CTA

   Promoter

   hUbC

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV hUbC-dCas9 VP64-T2A-GFP

   Plasmid

   #53192

   Purpose

   Co-expresses human optimized S. pyogenes dCas9-VP64 and GFP

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Insert

   humanized dead Cas9 VP64 T2A GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D10A and H840A

   Promoter

   hUbC

   Available since

   June 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. Fuw-dCas9-dead Tet1CD-P2A-BFP

    Plasmid

    #108246

    Purpose

    Lentiviral vector to express dCas9-dead Tet1CD-P2A-tagBFP

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

    Insert

    dCas9-dead Tet1CD-HA-P2A-BFP
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    dCas9-D10A, dCas9-H840A, Tet1CD-H1672Y, Tet1CD-D1…

    Promoter

    Ubc

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-CAG-DDdCas9VPH-T2A-GFP-IRES-Neo

    Plasmid

    #102886

    Purpose

    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC18-mini-Tn7T-Lac-dCas9 (Ptac)

    Plasmid

    #105234

    Purpose

    Tn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Ptac promoter for expression in P. putida and P. fluorescens

    Depositor

    Kristala Prather

    Article

    Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

    Insert

    S. pasteurianus dCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    HA

    Expression

    Bacterial

    Mutation

    D10A, H599A

    Promoter

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE-TO-nls-st1dCas9-3nls-3XGFP-2nls

    Plasmid

    #64113

    Purpose

    Expression of St1 dCas9-3XGFP in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XsfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. dSV40-NLS-dCas9-HA-NLS-NLS-10xGCN4

    Plasmid

    #107310

    Purpose

    Encoding dCas9-10xGCN4 driven by dSV40 promoter

    Depositor

    Hui Yang

    Article

    Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

    Insert

    dSV40-dCas9-10xGCN4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR-EF1a-dCas9-HA-BFP-KRAB-NLS

    Plasmid

    #102244

    Purpose

    Lentiviral expression of dCas9-HA-BFP-KRAB-NLS for CRISPRi driven by a hEF1alpha promoter

    Depositor

    Jacob Corn

    Insert

    dCas9-HA-BFP-KRAB-NLS
    

    Use

    CRISPR and Lentiviral

    Tags

    HA-TagBFP-KRAB-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC18-mini-Tn7T-Plac-dCas9 (Plac)

    Plasmid

    #105235

    Purpose

    Tn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Plac promoter for expression in P. aeruginosa

    Depositor

    Kristala Prather

    Article

    Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

    Insert

    S. pasteurianus dCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    HA

    Expression

    Bacterial

    Mutation

    D10A, H599A

    Promoter

    Available since

    Aug. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. CAG-DDdCas9VP192-T2A-EGFP-ires-puro

    Plasmid

    #69534

    Purpose

    DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

    Insert

    DD-dCas9VP192-T2A-EGFP
    

    Use

    Tags

    C-term T2A-EGFP and DHFR Destabilised Domain

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-TO-nls-st1dCas9-3nls-3XGFP-nls

    Plasmid

    #64112

    Purpose

    Expression of St1 dCas9-3XGFP in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XsfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB-CAG-DDdCas9VP192-T2A-GFP-IRES-Neo

    Plasmid

    #102885

    Purpose

    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PB-CAG-DDdCas9VPP300-T2A-GFP-IRES-Neo

    Plasmid

    #102887

    Purpose

    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-P300-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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