-
Plasmid#72460PurposeProduces Treponema denticola ATCC 35405 PurE with an N-terminal His6 tag (H6TdPurE2)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_1HKA
Plasmid#234169PurposeHeterologous protein expression of 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase in Escherichia coliDepositorInsert1HKA (folK Escherichia coli)
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJK547
Plasmid#72449PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide synthetase, Val319>Phe mutant (AaPurK-V319F)DepositorInsertN5-carboxyaminoimidazole ribonucleotide synthetase
UseTagsExpressionBacterialMutationVal319 to PhePromoterT7Available sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK550
Plasmid#72450PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with inserted Val2 (AaPurE1-V2ins)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationinserted Val2PromoterT7Available sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
lenti-RfxCas13d-Blast-BFP
Plasmid#196726PurposeSortable and selectable RfxCas13d.DepositorInsertRfxCas13d-T2A-BSD-TagBFP
UseCRISPR and LentiviralTagsSV40 NLSExpressionMutationPromoterEF1aAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJRD215
Plasmid#167555PurposeBackbone plasmid to pRMA154DepositorTypeEmpty backboneUseBroad host range mobilisableTagsExpressionMutationPromoterAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLL1270
Plasmid#129281PurposeThe vector used for enzyme expression in Thermoanaerobacterium. thermosaccharolyticum throughout this study. Contains the CBP promoter from Clostridium thermocellumDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterClostridium thermocellum DSM1313 Cellobiose Phosp…Available sinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE502B
Plasmid#128971PurposeBroad-host-range GenR plasmid expressing PapRecT and EcMutL_E32K with Toluic acid inductionDepositorInsertsPapRecT
mutL E32K
UseTagsExpressionBacterialMutationE32KPromoterxyl-PmAvailable sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcB6-Myc-Mfn2 IYFFT
Plasmid#191953PurposeExpression of Mfn2 protein, in which the C-terminus, after the transmembrane domain, is replaced by a stretch of hydrophobic amino acids (IYFFT)DepositorInsertMyc-Mfn2-IYFFT
UseTagsExpressionMammalianMutationC- terminus replaced by the stretch of hydrophobi…PromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-Reps1-EH domain
Plasmid#36860DepositorInsertReps1 (REPS1 Human)
UseTagsGSTExpressionBacterialMutationdeleted amino acids 1-225 and 315-769PromoterAvailable sinceJuly 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE302A
Plasmid#128970PurposeBroad-host-range KanR plasmid expressing PapRecT and EcMutL_E32K with high temperature inductionDepositorInsertsPapRecT
mutL E32K
UseTagsExpressionBacterialMutationE32KPromotercI-pLAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJK323
Plasmid#73495PurposeProduces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes and N5-carboxyaminoimidazole ribonucleotide synthetaseDepositorInsertshypothetical protein
N5-carboxyaminoimidazole ribonucleotide synthetase
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK322
Plasmid#73494PurposeProduces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes and N5-carboxyaminoimidazole ribonucleotide mutaseDepositorInsertshypothetical protein
N5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationPromotern/aAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK553
Plasmid#72452PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal His6 tag (H6AaPurE1)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsHis6ExpressionBacterialMutationsynthetic gene with ~40 silent substitutionsPromoterT7Available sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK636
Plasmid#72466PurposeProduces Treponema denticola ATCC 35405 PurE with a TEV protease-cleavable N-terminal His6 tag (H6tTdPurE2)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTP223
Plasmid#13263DepositorInsertPlac-gam-bet-exo
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSA_0_mKate2_synCoTC
Plasmid#199469PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertmKate2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSA_2_mKate2_synCoTC
Plasmid#199471PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertmKate2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSA_2_mTagBFP2_synCoTC
Plasmid#199474PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertmTagBFP2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSA_0_mTagBFP2_synCoTC
Plasmid#199472PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertmTagBFP2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only