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We narrowed to 3,270 results for: Streptococcus

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Showing: 2581 - 2600 of 3270 results

  1. pDual_dsCas9_Venus_hs_NC_chr2

    Plasmid
    #214683
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human non-coding DNA region of chromosome 2 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr2
    Use
    CRISPR and Lentiviral
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214683-plasmid-map-sequence-id-428804

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  2. pDual_dsCas9_Puro_hs_NC_chr1

    Plasmid
    #214688
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr1
    Use
    CRISPR and Lentiviral
    Mutation
    Puromycin resistance cassette has silent mutation…
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214688-plasmid-map-sequence-id-428817

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  3. pAG1guide_RUBY_EggCas9

    Plasmid
    #225983
    Purpose
    To make mutant alleles of AtAGAMOUS1 gene in Arabidopsis thaliana. The transgenics can be selected by red color appearance of plants
    Depositor
    Imran Siddiqi
    Insert
    Guide RNA against AtAGAMOUS1
    Use
    CRISPR
    Expression
    Plant
    Promoter
    35s promoter to Drive RUBY and DD45p to drive Cas9
    Available since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    225983-plasmid-map-sequence-id-448020

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  7. pLenti-nSpCas9-NoCBE

    Plasmid
    #209038
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized SpCas9(D10A)-6xNLS (Cas9 nickase) linked to ssDNA binding domain from RAD51 and uracil DNA glycosylase inhibitor (UGI)
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Publication
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209038-plasmid-map-sequence-id-413620

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  8. pLenti-nSpCas9-BEACON2

    Plasmid
    #209041
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where BEACON2 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Publication
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    BEACON2-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209041-plasmid-map-sequence-id-419488

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  9. pLenti-nSpCas9-TadA8.20m

    Plasmid
    #209045
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized adenine base editor where TadA8.20m is fused to the N-terminus of SpCas9(D10A)-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Publication
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    TadA8.20m-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209045-plasmid-map-sequence-id-413618

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  11. AAVS1-iPE2-Hygro

    Plasmid
    #214019
    Purpose
    inducible PE2 system for controllable prime editing; this plasmid is used to insert PE2 prime editor in one allele of AAVS1 locus
    Depositor
    Ting Zhou
    Publication
    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE2
    Expression
    Mammalian
    Promoter
    TRE-tight
    Available since
    June 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    214019-plasmid-map-sequence-id-429369

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  12. pSCBE3-NG

    Plasmid
    #218159
    Purpose
    This plasmid harbors the base editor SCBE3-NG along with an sgRNA cloning cassette, facilitating cytosine base editing at targets bearing NG PAM in Streptomyces.
    Depositor
    Yuhui Sun
    Publication
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    scbe3-NG
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutations in the portion of SpCas9 : D10A / L1111…
    Promoter
    rpsL promoter
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218159-plasmid-map-sequence-id-433199

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  13. peSCBE3-NG-HF1

    Plasmid
    #218164
    Purpose
    This plasmid harbors the base editor eSCBE3-NG-HF1 along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.
    Depositor
    Yuhui Sun
    Publication
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    escbe3-NG-HF1
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutation in the portion of deaminase hAPOBEC3A : …
    Promoter
    rpsL promoter
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218164-plasmid-map-sequence-id-433171

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  14. AA063

    Plasmid
    #216020
    Purpose
    Fragmid fragment: (Cas protein) nickase Cas for prime editing
    Depositor
    John Doench
    Publication
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nCas9-SpRY (H840A)_v1.1 [Sp]
    Use
    CRISPR; Fragment
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216020-plasmid-map-sequence-id-431526

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  16. AA007

    Plasmid
    #216002
    Purpose
    Fragmid fragment: (Cas protein) canonical Cas9; NGG PAM
    Depositor
    John Doench
    Publication
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9_v1.1 [Sp]
    Use
    CRISPR; Fragment
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216002-plasmid-map-sequence-id-431498

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  17. AA183

    Plasmid
    #216025
    Purpose
    Fragmid fragment: (Cas protein) for prime editing; R221K;N394K for increased efficiency
    Depositor
    John Doench
    Publication
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nCas9 (H840A)_v2.1 [Sp]
    Use
    CRISPR; Fragment
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216025-plasmid-map-sequence-id-431472

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Showing: 2581 - 2600 of 3270 results