Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 9,532 results for: SUB

Showing: 6501 - 6550 of 9532 results

  11. pCri System

    Plasmid Kit
    #1000000058
    Purpose
    pCri System: plasmids for cytoplasmic and periplasmic/extracellular heterologous protein expression in Escherichia coli, Bacillus subtilis, and Pichia pastoris.
    Depositor
    F Xavier Gomis-Ruth
    Available since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. FX cloning Kit

    Plasmid Kit
    #1000000039
    Purpose
    Insert sequence of interest into a cloning vector and subclone into expression vectors, containing a variety of N- and C-terminal tags; for use in both prokaryotic and eukaryotic systems
    Depositor
    Eric Geertsma
    Available since
    April 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. TRUPATH

    Plasmid Kit
    #1000000163
    Purpose
    A collection of 20 plasmids encoding alpha, beta, or gamma subunits of the heterotrimeric G protein complex for detection of heterotrimer dissociation. GPCR
    Depositor
    Bryan Roth
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only

  14. pJC224

    Plasmid
    #231169
    Purpose
    Empty Module A plasmid compatible with MoClo and Cermak (2017) genome engineering plasmids
    Depositor
    Daniel Voytas
    Type
    Empty backbone
    Use
    Subcloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    231169-plasmid-map-sequence-id-460659

    Enlarge

  15. pJC225

    Plasmid
    #231170
    Purpose
    Empty Module B plasmid compatible with MoClo and Cermak (2017) genome engineering plasmids
    Depositor
    Daniel Voytas
    Type
    Empty backbone
    Use
    Subcloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    231170-plasmid-map-sequence-id-459161

    Enlarge

  16. pJC227

    Plasmid
    #231172
    Purpose
    Empty Module C' plasmid compatible with MoClo and Cermak (2017) genome engineering plasmids
    Depositor
    Daniel Voytas
    Type
    Empty backbone
    Use
    Subcloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    231172-plasmid-map-sequence-id-459157

    Enlarge

  17. pHyVec11

    Plasmid
    #34794
    Depositor
    Rob Steele
    Type
    Empty backbone
    Use
    Hydra expression
    Tags
    Intergenic region between Hydra replication facto…
    Expression
    Mutation
    Promoter
    Hydra vulgaris actin promoter
    Available since
    Jan. 25, 2012
    Availability
    Academic Institutions and Nonprofits only
    34794-plasmid-map-sequence-id-41836

    Enlarge

  18. pEYFP-C1-p85beta

    Plasmid
    #1408
    Depositor
    Lewis Cantley
    Insert
    PI3K regulatory subunit p85beta (Pik3r2 Mouse)
    Use
    Tags
    eYFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 22, 2006
    Availability
    Academic Institutions and Nonprofits only

  19. pCDNA3-p110alpha-HA

    Plasmid
    #1397
    Depositor
    Lewis Cantley
    Insert
    PI3K catalytic subunit p110-alpha (PIK3CA Bovine)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2005
    Availability
    Academic Institutions and Nonprofits only

  20. pHM6-HA-p55gamma-His

    Plasmid
    #1396
    Depositor
    Lewis Cantley
    Insert
    PI3K regulatory subunit p55gamma (Pik3r3 Mouse)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 16, 2005
    Availability
    Academic Institutions and Nonprofits only

  21. pCR2.1-p55alpha

    Plasmid
    #1409
    Depositor
    Lewis Cantley
    Insert
    PI3K regulatory subunit p55alpha (Pik3r1 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 16, 2005
    Availability
    Academic Institutions and Nonprofits only

  22. pHyVec7

    Plasmid
    #34793
    Depositor
    Rob Steele
    Type
    Empty backbone
    Use
    Hydra expression
    Tags
    GFP, RFP, and intergenic region between Hydra rep…
    Expression
    Mutation
    Promoter
    Hydra vulgaris actin promoter
    Available since
    Jan. 25, 2012
    Availability
    Academic Institutions and Nonprofits only
    34793-plasmid-map-sequence-id-41832

    Enlarge

  23. pcDNA3.1-CnA-H151A

    Plasmid
    #191956
    Purpose
    Expression of phosphatase dead mutant of calcineurin A (CnA-H151A).
    Depositor
    Luca Scorrano
    Insert
    Calcineurin subunit alpha H151A
    Use
    Tags
    Expression
    Mammalian
    Mutation
    H151A
    Promoter
    Available since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    191956-plasmid-map-sequence-id-379808

    Enlarge

  24. pDONR223 MitoFlux (pSu9-HaloTag7-mGFP-STOP)

    Plasmid
    #215001
    Purpose
    Shuttling vector for a mitophagy reporter
    Depositor
    Wade Harper
    Insert
    pSu9 (Presequence of Fo-ATPase subunit 9)
    Use
    Gateway
    Tags
    HaloTag(7)-mGFP
    Expression
    Mutation
    Promoter
    Available since
    July 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    215001-plasmid-map-sequence-id-425587

    Enlarge

  25. pBS-p85alpha

    Plasmid
    #1407
    Depositor
    Lewis Cantley
    Insert
    PI3K regulatory subunit p85alpha (Pik3r1 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 16, 2005
    Availability
    Academic Institutions and Nonprofits only

  26. pGEX-4T3-p85beta

    Plasmid
    #1406
    Depositor
    Lewis Cantley
    Insert
    PI3K regulatory subunit p85beta (Pik3r2 Mouse)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 14, 2005
    Availability
    Academic Institutions and Nonprofits only

  27. pIREShyg-p50alpha-Flag

    Plasmid
    #1405
    Depositor
    Lewis Cantley
    Insert
    PI3K regulatory subunit p50alpha (Pik3r1 Mouse)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 16, 2005
    Availability
    Academic Institutions and Nonprofits only

  28. pMIMY

    Plasmid
    #191954
    Purpose
    A pMSCV-based backbone where the MCS has been substituted with a MCS-IRES-mitoYFP for expression of a gene of interest and a mitochondrially targeted YFP
    Depositor
    Luca Scorrano
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    191954-plasmid-map-sequence-id-379817

    Enlarge

  29. pCMV6-p85alpha-Flag

    Plasmid
    #1399
    Depositor
    Lewis Cantley
    Insert
    PI3K regulatory subunit p85alpha (Pik3r1 Mouse)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2006
    Availability
    Academic Institutions and Nonprofits only
    1399-plasmid-map-sequence-id-340532

    Enlarge

  30. pCMV6-p55alpha-Flag

    Plasmid
    #1402
    Depositor
    Lewis Cantley
    Insert
    PI3K regulatory subunit p55alpha (Pik3r1 Mouse)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2006
    Availability
    Academic Institutions and Nonprofits only

  31. pOpen-ALPI

    Plasmid
    #165555
    Purpose
    CIAP reporter; can be used in spectrophotometric or luminescence assay when appropriate substrate is used
    Depositor
    Jennifer Molloy, FreeGenes Project, Drew Endy
    Insert
    CIP (calf intestinal phosphatase)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165555-plasmid-map-sequence-id-339907

    Enlarge

  32. pOpen-9N7polA (CT)

    Plasmid
    #165502
    Purpose
    9 Degrees North DNA polymerase variant with enhanced ability to incorporate modified substrates
    Depositor
    Jennifer Molloy, FreeGenes Project, Drew Endy
    Insert
    9 degrees N-7 DNA Polymerase chain terminating
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165502-plasmid-map-sequence-id-338438

    Enlarge

  34. OsMATE

    Plasmid
    #117093
    Purpose
    protein expression in yeast
    Depositor
    Geoffrey Chang
    Insert
    AtTPT (ORF-HIS)
    Use
    Tags
    His
    Expression
    Yeast
    Mutation
    Promoter
    AOX
    Available since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    117093-plasmid-map-sequence-id-224955

    Enlarge

  35. C85m

    Plasmid
    #120996
    Purpose
    MoClo golden gate assembly CD part for sigmaX (Bacillus subtilis RNA polymerase involved in heat response and biofilm formation). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Sigma X (ECF20_992)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120996-plasmid-map-sequence-id-236125

    Enlarge

  36. pGR420

    Plasmid
    #213782
    Purpose
    Integrates onto p1 a partial LEU2 sequence to be subsequently used as an integration flank
    Depositor
    Chang Liu
    Insert
    MET15
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    213782-plasmid-map-sequence-id-424626

    Enlarge

  37. pLenti UBC MitoFlux (pSu9-HaloTag7-mGFP-STOP)

    Plasmid
    #215010
    Purpose
    Express a Mitophagy reporter in mammalian cells (untested)
    Depositor
    Wade Harper
    Insert
    pSu9 (Presequence of Fo-ATPase subunit 9)
    Use
    Lentiviral
    Tags
    HaloTag(7)-mGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    215010-plasmid-map-sequence-id-429275

    Enlarge

  38. pOpen-T3gene1

    Plasmid
    #165576
    Purpose
    DNA-dependent RNA polymerase derived from T3 bacteriophage that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
    Depositor
    Jennifer Molloy, FreeGenes Project, Drew Endy
    Insert
    T3 RNA Polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165576-plasmid-map-sequence-id-340988

    Enlarge

  40. pSiM1

    Plasmid
    #199380
    Purpose
    Encodes dual cistronic membrane localized GCaMP6s and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    GCaMP6s::ras-2CAAX::SL2::mScarlet-I::ras-2CAAX
    Use
    Tags
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Promoter
    Available since
    April 24, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199380-plasmid-map-sequence-id-391037

    Enlarge

  41. pSiM4

    Plasmid
    #199383
    Purpose
    Encodes dual cistronic membrane localized jGCaMP7f and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    jGCaMP6f::ras-2CAAX::SL2::mScarlet-I::ras-2CAAX
    Use
    Tags
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Promoter
    Available since
    April 24, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199383-plasmid-map-sequence-id-391021

    Enlarge

  42. pSiM3

    Plasmid
    #199382
    Purpose
    Encodes dual cistronic membrane localized jGCaMP7s and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    jGCaMP7s::ras-2CAAX::SL2::mScarlet-I::ras-2CAAX
    Use
    Tags
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Promoter
    Available since
    April 24, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199382-plasmid-map-sequence-id-391043

    Enlarge

  43. SnFR_S074

    Plasmid
    #182811
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Insert
    S074-CholineSnFR
    Use
    Tags
    6xHis, HA and Myc
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    182811-plasmid-map-sequence-id-358311

    Enlarge

  44. SnFR_S062

    Plasmid
    #182810
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Insert
    S062-CholineSnFR
    Use
    Tags
    6xHis, HA and Myc
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    182810-plasmid-map-sequence-id-358310

    Enlarge

  45. Ec holD-pET(3c)

    Plasmid
    #237236
    Purpose
    over-express E. coli holD (Pol III psi subunit)
    Depositor
    Michael O'Donnell
    Insert
    holD (psi) (holD E. coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    237236-plasmid-map-sequence-id-469263

    Enlarge

  46. Ec holC-pET(3c)

    Plasmid
    #237235
    Purpose
    over-express E. coli holC (Pol III chi subunit)
    Depositor
    Michael O'Donnell
    Insert
    holC (chi) (holC E. coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    237235-plasmid-map-sequence-id-469249

    Enlarge

  47. pET41-FeoB-C675S

    Plasmid
    #216756
    Purpose
    Investigation of the potential interaction of the FeoB Cys675 residue with ferrous iron
    Depositor
    Rietie Venter
    Insert
    feoB (PA4358 P. aeruginosa (Bacteria))
    Use
    Tags
    8x His
    Expression
    Bacterial
    Mutation
    Amino acid C675 is substituted with S.
    Promoter
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    216756-plasmid-map-sequence-id-434497

    Enlarge

  48. pET41-FeoB-C429S

    Plasmid
    #216755
    Purpose
    Investigation of the potential interaction of the FeoB Cys429 residue with ferrous iron
    Depositor
    Rietie Venter
    Insert
    feoB (PA4358 P. aeruginosa (Bacteria))
    Use
    Tags
    8x His
    Expression
    Bacterial
    Mutation
    Amino acid C429 is substituted with S.
    Promoter
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    216755-plasmid-map-sequence-id-434494

    Enlarge

  49. pET41-FeoB-D123N

    Plasmid
    #216754
    Purpose
    Production of a FeoB that is unable to bind GTP for a negative control in GTPases assays
    Depositor
    Rietie Venter
    Insert
    feoB (PA4358 P. aeruginosa (Bacteria))
    Use
    Tags
    8x His
    Expression
    Bacterial
    Mutation
    Amino acid D123 is substituted with N.
    Promoter
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    216754-plasmid-map-sequence-id-434490

    Enlarge

  50. pHAGE pGK MitoFlux (pSu9-HaloTag7-mGFP-STOP)

    Plasmid
    #215011
    Purpose
    Express a Mitophagy reporter in mammalian cells (untested)
    Depositor
    Wade Harper
    Insert
    pSu9 (Presequence of Fo-ATPase subunit 9)
    Use
    Lentiviral
    Tags
    HaloTag(7)-mGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    215011-plasmid-map-sequence-id-429274

    Enlarge

Showing: 6501 - 6550 of 9532 results