We narrowed to 31,194 results for: uros

1. pPuroR-MCS-LexA-VP16-MiniWhite

   Plasmid

   #165898

   Purpose

   Puromycin resistant LexA driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloning

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Selectable Empty Enhancer Driver
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. attB-mCh-Dnmt3b1-Poly(A)-PuroR

   Plasmid

   #65556

   Purpose

   Plasmid for Bxb1-mediated recombination of the mCh-Dnmt3b1 cDNA into a MIN-tagged locus using Puromycin selection

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   Dnmt3b (Dnmt3b Mouse)
   

   Use

   Mouse Targeting; Bxb1

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   isoform 1

   Promoter

   Available since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pIVTR(T7A)-Cas9n-RT-T2A-PuroR

   Plasmid

   #172296

   Purpose

   In vitro transcription of Cas9n-RT-T2A-PuroR synthetic construct for PRIME editing

   Depositor

   Patrik Verstreken

   Insert

   Cas9n-RT-T2A-PuroR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   H840A (Cas9)

   Promoter

   T7 promoter A-initiating

   Available since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLKO.1-puro U6 sgRNA Oct4A -158

   Plasmid

   #50922

   Purpose

   U6 driven sgRNA targeting OCT4 isoform A -158 bp from TSS

   Depositor

   Rene Maehr, Scot Wolfe

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Insert

   Oct4A -158 sgRNA (POU5F1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. 970 pBabe puroL PTEN R130G

   Plasmid

   #10783

   Depositor

   William Sellers

   Insert

   PTEN (PTEN Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   R130G

   Promoter

   Available since

   Sept. 27, 2005

   Availability

   Academic Institutions and Nonprofits only

6. pRetro-XT-PAK2-CFP(puro)-L107F

   Plasmid

   #81180

   Purpose

   Retroviral vector for expression of kinase active PAK2 with a CFP fusion

   Depositor

   Angeliki Malliri

   Article

   Whalley et al Nat Commun. 2015 Jun 16;6:7437. doi: 10.

   Insert

   p21 (RAC1) activated kinase 2 (PAK2 Human)
   

   Use

   Retroviral

   Tags

   CFP

   Expression

   Mutation

   L107F

   Promoter

   P-tight TRE

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

7. pBabe puro-HA-PIK3CA(H1047R;G320E)

   Plasmid

   #33254

   Depositor

   Thomas Roberts

   Article

   Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

   Insert

   PIK3CA (PIK3CA Human)
   

   Use

   Retroviral

   Tags

   Hemagglutinin (HA)

   Expression

   Mammalian

   Mutation

   changed histidine 1047 to arginine and glycine 32…

   Promoter

   SV40

   Available since

   Jan. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pBMN-puro-P2A-FRB-Fis1

   Plasmid

   #135297

   Purpose

   stably express FBR-Fis1 in mammalian cells with puromycin selection

   Depositor

   Richard Youle

   Article

   Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

   Insert

   FRB-Fis1 (FIS1 Human)
   

   Use

   Retroviral

   Tags

   FRB

   Expression

   Mammalian

   Mutation

   Fis1 tail (aa 93-152)

   Promoter

   Available since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Cenp-B-targeted Aurora B FRET sensor

   Plasmid

   #45232

   Depositor

   Michael Lampson

   Article

   Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

   Insert

   CENP-B targeted Aurora B FRET sensor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   t7

   Available since

   May 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGL3-sgACSL3.C-Cas9-P2A-Puro

    Plasmid

    #129412

    Purpose

    Encoding Cas9 and sgACSL3.C for CRISPR/Cas9 mediated HDR tagging of endogenous human ACSL3 C-terminus

    Depositor

    Elina Ikonen

    Article

    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

    Insert

    SpCas9 and sgACSL3.C (ACSL3 Human)
    

    Use

    CRISPR

    Tags

    P2A-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBabe-puro-miR-10b sponge

    Plasmid

    #25043

    Depositor

    Bob Weinberg

    Article

    Ma et al Nat Biotechnol. 2010 Apr . 28(4):341-7.

    Insert

    miR-10b sponge
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pSUPER retro puro macroH2A1 shRNA

    Plasmid

    #30517

    Depositor

    John Gurdon

    Article

    Pasque et al EMBO J. 2011 May 6. ():.

    Insert

    mH2A1 shRNA
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. qTAG-C-moxGFP-Puro-H2BC11-MMEJ

    Plasmid

    #207760

    Purpose

    MMEJ donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H2BC11 Short Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pCDH-puro/Flag PD-L1 N35/3NQ

    Plasmid

    #121455

    Purpose

    expression of human PD-L1 mutant in mammalian cells

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    CD274 (CD274 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N192Q, N200Q and N219Q

    Promoter

    CMV

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Puro-T2A-mCitrine-hSmad2-donor

    Plasmid

    #203663

    Purpose

    Donor plasmid for CRISPR knock-in

    Depositor

    Ali Hemmati Brivanlou

    Article

    Yoney et al Elife. 2018 Oct 12;7:e38279. doi: 10.755

    Insert

    Smad2
    

    Use

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pME-puro-CLIP-FLAG-CD59

    Plasmid

    #50375

    Purpose

    Express CLIP and FLAG-tagged human CD59, a GPI-AP in mammmalian cells

    Depositor

    Reika Watanabe

    Article

    Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

    Insert

    CLIP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SR alpha

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. integrin beta6 Y774F pBabe puro

    Plasmid

    #13599

    Depositor

    Dean Sheppard

    Insert

    integrin beta 6 Y774F (ITGB6 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Y774F

    Promoter

    Available since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

18. integrin alpha9 EGFP pBabe puro

    Plasmid

    #13605

    Depositor

    Dean Sheppard

    Insert

    integrin alpha 9 (ITGA9 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pSicoR-Ef1a-mCh-Puro-GFPi

    Plasmid

    #31848

    Depositor

    Bruce Conklin

    Article

    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

    Insert

    GFP RNAi
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA; Ef1alpha for mCherry-puromycin

    Available since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBABE-2xFLAG-2xSTREP-CCND3-Puro

    Plasmid

    #172622

    Purpose

    Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D3 and a puromycin resistance marker in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND3 (CCND3 Human)
    

    Use

    Retroviral

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge