We narrowed to 4,276 results for: 424

1. PLOD2-bio-His

   Plasmid

   #51756

   Purpose

   Expresses full-length Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   PLOD2 (PLOD2 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. WT A1-LCD +NLS

   Plasmid

   #178658

   Purpose

   Bacterial expression of N-terminally 6His tagged WT A1-LCD (LCD: low-complexity domain, aa 186-320)

   Depositor

   Tanja Mittag

   Article

   Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

   Insert

   WT A1-LCD +NLS (HNRNPA1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-FLAG-HA-CAD S1859A

   Plasmid

   #46239

   Purpose

   FLAG-HA-CAD with S1859 phosphorylation site mutated to alanine

   Depositor

   Brendan Manning

   Article

   Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. d

   Insert

   carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Serine 1859 changed to Alanine (S1859A)

   Promoter

   cmv

   Available since

   Nov. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pEBTet-SNAP-Cep63

   Plasmid

   #136811

   Purpose

   Mammalian expression of the centrosomal protein Cep63 N-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   SNAP-Cep63 (CEP63 Synthetic, Human)
   

   Use

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TetO2

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBetaActin-FKBP-mScarlet-Dync1h1motor

   Plasmid

   #191330

   Purpose

   Expresses the fluorescently labeled dynein motor domain fused to dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

   Depositor

   Frank Bradke

   Article

   Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

   Insert

   FKBP-mScarlet-Dync1h1motor (Dync1h1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   DYNC1H1 motor domain aa 1453-4644

   Promoter

   Available since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. psd44-iGFP-MFGE8-long

   Plasmid

   #46847

   Purpose

   expression of MFGE8 in mammalian cells

   Depositor

   Agnese Mariotti

   Article

   Carrascosa et al Oncogene. 2012 Mar 22;31(12):1521-32. do

   Insert

   MFGE8 (Mfge8 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK promoter

   Available since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pET21a-C2m2-mKate

   Plasmid

   #208765

   Purpose

   To express C2m2-mKate in bacteria

   Depositor

   Urte Neniskyte

   Article

   Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

   Insert

   C2m2-mKate (Mfge8 Mouse)
   

   Use

   Tags

   mKate

   Expression

   Bacterial

   Mutation

   Mutated 24 and 45 lysine to asparagine in C2 doma…

   Promoter

   T7 promoter

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. EGFP-SopB

   Plasmid

   #183657

   Purpose

   N-terminally tagged Salmonella Typhimurium SopB for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEF-Bos-FLAG MDA5 MIII

   Plasmid

   #52876

   Purpose

   Expresses MDA5 with a mutation to motif III in mammalian cells

   Depositor

   Curt Horvath

   Article

   Bamming et al J Biol Chem. 2009 Apr 10;284(15):9700-12

   Insert

   IFIH1 (IFIH1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   T488A S490A

   Promoter

   Available since

   May 9, 2014

   Availability

   Academic Institutions and Nonprofits only

10. MiniCoopR mitfa:NRAS

    Plasmid

    #118847

    Purpose

    Expresses human NRAS Q61R mutant and zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Insert

    NRAS Q61R (NRAS Zebrafish, Human)
    

    Use

    Zebrafish expression, tol3

    Tags

    Expression

    Mutation

    NRAS Q61R

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. GPR35-Tango

    Plasmid

    #66354

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR35 (GPR35 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pPBbsr2-Gnaq

    Plasmid

    #170298

    Purpose

    Encoding mouse Gnaq

    Depositor

    Michiyuki Matsuda

    Article

    Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

    Insert

    A recombinant mouse Gnaq (Gnaq Mouse)
    

    Use

    Tags

    N/A

    Expression

    Mammalian

    Mutation

    Asilent mutation was introduced into the sgRNA-ta…

    Promoter

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEBTetD-SNAP-Cep63

    Plasmid

    #136812

    Purpose

    Mammalian expression of the centrosomal protein Cep63 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-Cep63 (CEP63 Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. lentiCRISPR v2-sgACSL4

    Plasmid

    #186024

    Purpose

    knock out ACSL4 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Mao et al Nature. 2021 May;593(7860):586-590. doi:

    Insert

    Acyl-CoA Synthetase Long Chain Family Member 4 (ACSL4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLEX-FHH-NRASQ61K-IRES-Puro

    Plasmid

    #120570

    Purpose

    Expresses Flag-HA-6xHIS-N-Ras Q61K fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    Flag-HA-6xHIS

    Expression

    Mammalian

    Mutation

    Q61K

    Promoter

    CMV

    Available since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. SNAP-ACE2

    Plasmid

    #178592

    Purpose

    Expresses SNAP-fused ACE2 protein that can be fluorescently labelled using SNAP substrates.

    Depositor

    Ralf Jockers

    Article

    Cecon et al Cell Chem Biol. 2021 Jul 2. pii: S2451-9

    Insert

    angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Flag and SNAP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLEX-FHH-NRAS-IRES-Puro

    Plasmid

    #120569

    Purpose

    Expresses Flag-HA-6xHIS-N-Ras wildtype fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    Flag-HA-6xHIS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GPR1-Tango

    Plasmid

    #66300

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR1 (GPR1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

19. Flag-Jun4A-Myc

    Plasmid

    #47444

    Purpose

    mammalian expression of mutant c-Jun (four N-terminal residues phosphorylated by JNK are mutated into Alanines)

    Depositor

    Axel Behrens

    Article

    Aguilera et al Nature. 2011 Jan 13;469(7329):231-5. doi

    Insert

    Jun 4A mutant (Jun Mouse)
    

    Use

    Tags

    Flag and myc

    Expression

    Mammalian

    Mutation

    four N-terminal residues phosphorylated by JNK, s…

    Promoter

    CMV

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. His6tev CBP BCHZ (1079-1751)

    Plasmid

    #99341

    Purpose

    Bacterial expression of His tagged mouse CBP HAT core Brd-ZZ (1079-1751)

    Depositor

    Peter Wright

    Article

    Park et al Proc Natl Acad Sci U S A. 2017 Jun 19. p

    Insert

    mouse CBP BRD-ZZ domains (1079-1751) (Crebbp Mouse)
    

    Use

    Tags

    His6tev

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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