We narrowed to 32,166 results for: uros

1. pLKO-Tet-puro-hRAF1-shRNA-1

   Plasmid

   #185371

   Purpose

   For tetracycline-inducible mammalian expression of shRNA: CATGAGTATTTAGAGGAAGTA that targets human RAF1

   Depositor

   Ayaz Najafov

   Insert

   RAF1 (RAF1 Human)
   

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSCV-FLAG-2xSTREP-CCND3-Puro

   Plasmid

   #172619

   Purpose

   Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3 and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND3 (CCND3 Human)
   

   Use

   Retroviral

   Tags

   FLAG-2xSTREP

   Expression

   Mammalian

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. 911 pBabe puroL PTEN G129E

   Plasmid

   #10771

   Depositor

   William Sellers

   Insert

   PTEN (PTEN Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   G129E

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

4. AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)_FS

   Plasmid

   #191527

   Purpose

   Donor for targeted integration of 3xFLAG-2xSTREP tagged S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locus

   Depositor

   Yannick Doyon

   Insert

   hGEM(1-110)_mNG_hSLBP(18-108)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   mNeonGreen (mNG) + 3xFLAG_TwinStrep

   Expression

   Mammalian

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-CMVSP6-NR2F2-SV40-PURO

   Plasmid

   #138361

   Purpose

   Lentiviral plasmid contains human NR2F2 driven by CMVSP6 and puromycin resistance for selection

   Depositor

   Nathan Lawson

   Article

   Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

   Insert

   Human NR2F2, with puromycin driven by SV40 for selection
   

   Expression

   Mammalian

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. ePB-PURO-FLAG-METTL3-APPA

   Plasmid

   #160253

   Purpose

   For stable integration of catalytic inactive FLAG-METTL3 in human cells with trasposase

   Depositor

   Alessandro Fatica

   Article

   Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

   Insert

   methyltransferase like 3 (METTL3 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changes in aa 395–398, DPPW → APPA

   Promoter

   tight TRE promoter

   Available Since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDestTol2CG2-Neurog1-Magneto2.0-p2A-mCherry-pA

   Plasmid

   #74302

   Purpose

   Expression of Magneto2.0-p2A-mCherry in Rohon-Beard sensory neurons of the zebrafish

   Depositor

   Ali Guler

   Article

   Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

   Insert

   Magneto2.0 (FTL, FTH1, Trpv4 Human, Rat)
   

   Use

   Zebrafish

   Tags

   FLAG, cmcl2::EGFP, and p2A-mCherry

   Mutation

   TRPV4: delta 760-871

   Promoter

   Neurog1

   Available Since

   May 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. Pb459-mU6-sgRNA-EF1a-PuroR

   Plasmid

   #195507

   Purpose

   piggybac vector expressing non-targeting control sgRNA cloned using BlpI and BstXI sites

   Depositor

   Joanna Wysocka

   Article

   Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

   Insert

   Puromycin
   

   Use

   CRISPR; Piggybac

   Expression

   Mammalian

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. RRL.sin.cPPT.SFFV/Flag-E2-crimson.IRES-puro.WPRE (CG568)

   Plasmid

   #139445

   Purpose

   Lentiviral vector to ectopically express Flag-tagged E2-crimson from SFFV promoter

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   FLAG-tag E2-crimson
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKLV2-U6gRNA5(hWWTR1-W1K)-PGKpuro2AmCherry-W

    Plasmid

    #163176

    Purpose

    Lentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of mCherry tag

    Depositor

    Sok Ching Cheong

    Article

    Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

    Insert

    WWTR1 (WWTR1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLC-Flag-CSNK1A1 G40N-Puro

    Plasmid

    #123320

    Purpose

    Lentiviral vector for expression of Flag tagged CK1alpha G40N-P2A-Puro casette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

    Insert

    CSNK1A1 (CSNK1A1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed Glycine 40 to Asparagine

    Promoter

    CMV

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTopo pLox hPGK-Puro pA pLox

    Plasmid

    #171048

    Purpose

    Empty donor template for CRISPR/Cas9 targeting of TERT RE's

    Depositor

    Agnel Sfeir

    Article

    Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

    Type

    Empty backbone

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX puro TRIM21 C54Y - GFP

    Plasmid

    #116942

    Purpose

    Expression of TRIM21 C54Y -GFP fusion

    Depositor

    Gaudenz Danuser

    Article

    Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

    Insert

    TRIM21 C54Y-GFP (TRIM21 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    C54Y

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAVS1-Puro CAG IL13-CAR

    Plasmid

    #157742

    Purpose

    knock IL13-CAR into AAVS1 safe harbor locus for constitutive expression

    Depositor

    Xiaoping Bao

    Article

    Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

    Insert

    Chimeric antigen receptor targeting IL13
    

    Use

    CRISPR, Synthetic Biology, and TALEN

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLentiPGK Puro DEST JNKKTR EE Clover

    Plasmid

    #90239

    Purpose

    Lentiviral vector to express JNK KTR EE (mutant) mClover under PGK promoter (With Puromycin Resistance)

    Depositor

    Markus Covert

    Article

    Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

    Insert

    JNK Kinase Translocation Reporter (EE mutant)
    

    Use

    Lentiviral

    Tags

    mClover

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    March 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBABE puro Brca1 S1423A S1524A HA

    Plasmid

    #41968

    Depositor

    Stephen Elledge

    Article

    Cortez et al Science. 1999 Nov 5. 286(5442):1162-6.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S1423A, S1524A

    Available Since

    April 26, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pUC-DEST-hSOX2-EGFP-PuroTK

    Plasmid

    #141026

    Purpose

    Reporter integration

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    EGFP
    

    Expression

    Mammalian

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. LentiU6-hASCL1 gRNA_multi1-3-MS2-Puro

    Plasmid

    #192682

    Purpose

    Lentiviral expression of multi gRNAs targeting hASCL1 promoter to activate human ASCL1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Human ASCL1 activating gRNAs #1,2,3 (ASCL1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCT-CMV-UBE3B-copGFP-Puro

    Plasmid

    #107342

    Purpose

    Expresses UBE3B fused to copGFP in mammalian cells.

    Depositor

    Robert Sobol

    Article

    Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

    Insert

    Ubiquitin-protein ligase E3B (UBE3B Human)
    

    Use

    Lentiviral

    Tags

    copGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pBabe-TetCMV-puro-mVenus-LOV

    Plasmid

    #22033

    Purpose

    Mammalian expression of mVenus tagged LOV2 domain

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    mVenus-LOV
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Insert length based on Venus (720bp) + LOV (450bp)

    Available Since

    Sept. 11, 2009

    Availability

    Academic Institutions and Nonprofits only