234,697 results

1. pCMV-GCaMP5G

   Plasmid

   #31788

   Purpose

   a single-wavelength GCaMP3-based calcium indicator with improved response. Please also see the GCaMP6s/m/f indicators.

   Depositor

   GENIE Project, Douglas Kim, Loren Looger

   Article

   Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

   Insert

   GCaMP5G
   

   Use

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CAG-iSeroSnFR-Nlgn (AAV9)

   Viral Prep

   #128485-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-CAG-iSeroSnFR-Nlgn (#128485). In addition to the viral particles, you will also receive purified pAAV-CAG-iSeroSnFR-Nlgn plasmid DNA. CAG-driven expression of a fluorescent reporter for serotonin (localized to dendrites). These AAV preparations are suitable purity for injection into animals.

   Depositor

   Lin Tian

   Article

   Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

   Promoter

   CAG

   Tags

   None

   Available since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

3. CanlonicSF

   Plasmid

   #178342

   Purpose

   To explore lactate dynamics in intact systems

   Depositor

   Alejandro San Martín

   Article

   Aburto et al ACS Sens. 2022 Oct 28. doi: 10.1021/acss

   Insert

   CanlonicSF
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. MCS-3_Endofin_LgBiT

   Plasmid

   #182574

   Purpose

   This plasmid can be used to monitor receptor internalization in real time living cells using bioluminescence as a readout.

   Depositor

   Bradley McConnell

   Article

   Reyes-Alcaraz et al Commun Biol. 2022 Mar 8;5(1):212. doi: 1

   Insert

   zinc finger FYVE-type (ZFYVE16 Human)
   

   Use

   Tags

   LgBiT (Large BiT)

   Expression

   Mammalian

   Mutation

   FYVE domain Q739-K806

   Promoter

   Herpes simplex virus (HSV)

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcs2-HRI-GFP-IRES-mCherry

   Plasmid

   #226099

   Purpose

   HRI stability reporter construct for transient expression in mammalian cells

   Depositor

   Michael Rape

   Article

   Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

   Insert

   HRI (EIF2AK1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. RUSH reporter-HMGB1-SBP-GFP

   Plasmid

   #172357

   Purpose

   Monitoring of HMGB1 subcellular localization by fluorescence videomicroscopy

   Depositor

   Guido Kroemer

   Article

   Liu et al Sci Rep. 2017 Nov 2;7(1):14915. doi: 10.

   Insert

   high mobility group box 1 (HMGB1 Human)
   

   Use

   Lentiviral

   Tags

   SBP-GFP

   Expression

   Mutation

   Promoter

   CMV

   Available since

   June 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-hsyn-GRAB_rDA1m (AAV9)

   Viral Prep

   #140556-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-hsyn-GRAB_rDA1m (#140556). In addition to the viral particles, you will also receive purified pAAV-hsyn-GRAB_rDA1m plasmid DNA. Synapsin-driven expression of red fluorescent medium affinity dopamine sensor GRAB_rDA1m. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Yulong Li

   Article

   Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

   Promoter

   Syn

   Tags

   None

   Available since

   Aug. 31, 2021

   Availability

   Academic Institutions and Nonprofits only

8. pSFFV_sfCherry3C(1-10)

   Plasmid

   #117482

   Purpose

   Lentiviral vector for constitutive expression of sfCherry3C(1-10)

   Depositor

   Bo Huang

   Article

   Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

   Insert

   sfCherry3C(1-10)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   5 substitutions (K45R, G52D, T106A, K182R, N194D)…

   Promoter

   SFFV

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCM184-ParB2::eGFP

   Plasmid

   #87252

   Purpose

   Expresses ParB2-EGFP in yeast. Also known as pOR2-GFP

   Depositor

   Kerstin Bystricky

   Article

   Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

   Insert

   ParB2
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   pBAD/tetO

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCM184-ParB2::eGFP

    Plasmid

    #87252

    Purpose

    Expresses ParB2-EGFP in yeast. Also known as pOR2-GFP

    Depositor

    Kerstin Bystricky

    Article

    Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

    Insert

    ParB2
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    pBAD/tetO

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Flag-HA-USP33

    Plasmid

    #22601

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP33 (USP33 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Flag-HA-USP33

    Plasmid

    #22601

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP33 (USP33 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 4xM67 pTATA TK-Luc

    Plasmid

    #8688

    Depositor

    Jim Darnell

    Article

    Besser et al Mol Cell Biol 1999 Feb;19(2):1401-9.

    Insert

    4 repeated M67 sites
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 4xM67 pTATA TK-Luc

    Plasmid

    #8688

    Depositor

    Jim Darnell

    Article

    Besser et al Mol Cell Biol 1999 Feb;19(2):1401-9.

    Insert

    4 repeated M67 sites
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a-LSSmOrange-CNA35

    Plasmid

    #61605

    Purpose

    bacterial expression of N-terminal fusion protein of CNA35 with LSSmOrange

    Depositor

    Maarten Merkx

    Article

    Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

    Insert

    LSSmOrange and CNA35
    

    Use

    Tags

    His tag

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a-LSSmOrange-CNA35

    Plasmid

    #61605

    Purpose

    bacterial expression of N-terminal fusion protein of CNA35 with LSSmOrange

    Depositor

    Maarten Merkx

    Article

    Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

    Insert

    LSSmOrange and CNA35
    

    Use

    Tags

    His tag

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP (AAV5)

    Viral Prep

    #35509-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP (#35509). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP plasmid DNA. EF1a-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Promoter

    EF1a

    Tags

    EYFP (Cre-dependent)

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

18. piRFP-N3-Tyrosinase

    Plasmid

    #80152

    Purpose

    Melanosome marker

    Depositor

    Santiago Di Pietro

    Article

    Ambrosio et al Proc Natl Acad Sci U S A. 2016 May 17;11

    Insert

    Tyrosinase (TYR Human)
    

    Use

    Tags

    iRFP682

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. piRFP-N3-Tyrosinase

    Plasmid

    #80152

    Purpose

    Melanosome marker

    Depositor

    Santiago Di Pietro

    Article

    Ambrosio et al Proc Natl Acad Sci U S A. 2016 May 17;11

    Insert

    Tyrosinase (TYR Human)
    

    Use

    Tags

    iRFP682

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pTripZ RB1 WT-3XFLAG

    Plasmid

    #209070

    Purpose

    DOX-ON inducible expression vector that expresses RB1 WT with c-terminal 3X flag tag

    Depositor

    Matthew Oser

    Article

    Oser et al Cancer Discov. 2019 Feb;9(2):230-247. do

    Insert

    human RB1 WT after LR recombination with pTripZ NEO DEST to inducibly express RB1 WT with a c-terminal 3X flag tag
    

    Use

    Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. p5E-mfap4

    Plasmid

    #70052

    Purpose

    5' Entry vector containing the zebrafish mfap4 promoter. Used with Gateway Recombination to generate transgenic constructs exhibiting macrophage-restricted expression of transgenes.

    Depositor

    David Tobin

    Article

    Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

    Insert

    mfap4 promoter
    

    Use

    Gateway 5' element

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p5E-mfap4

    Plasmid

    #70052

    Purpose

    5' Entry vector containing the zebrafish mfap4 promoter. Used with Gateway Recombination to generate transgenic constructs exhibiting macrophage-restricted expression of transgenes.

    Depositor

    David Tobin

    Article

    Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

    Insert

    mfap4 promoter
    

    Use

    Gateway 5' element

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA-HDAC6.DC-FLAG

    Plasmid

    #30483

    Depositor

    Tso-Pang Yao

    Article

    Kawaguchi et al Cell. 2003 Dec 12. 115(6):727-38.

    Insert

    HDAC6 (HDAC6 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Catalytically inactive mutant (H216/611A)

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA-HDAC6.DC-FLAG

    Plasmid

    #30483

    Depositor

    Tso-Pang Yao

    Article

    Kawaguchi et al Cell. 2003 Dec 12. 115(6):727-38.

    Insert

    HDAC6 (HDAC6 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Catalytically inactive mutant (H216/611A)

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AAV-F in pAR-9

    Plasmid

    #166921

    Purpose

    AAV rep/cap plasmid. Capsid is mouse brain tropic AAV-F

    Depositor

    Casey Maguire

    Article

    Hanlon et al Mol Ther Methods Clin Dev. 2019 Oct 23;1

    Insert

    REP/CAP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLKO.1 puro shTEAD1/3/4-1

    Plasmid

    #193672

    Purpose

    Constitutive lentiviral expression of TEAD shRNA

    Depositor

    William Hahn

    Article

    Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

    Insert

    TEAD
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. hSyn-somBiPOLES-mCerulean

    Plasmid

    #154945

    Purpose

    Optogenetic tool for blue-light inhibition and red-light excitation of neurons

    Depositor

    Simon Wiegert

    Article

    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCM189-ParB1::mCherry

    Plasmid

    #87253

    Purpose

    Expresses ParB1-mCherry in yeast. Also known as pOR1-mch

    Depositor

    Kerstin Bystricky

    Article

    Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

    Insert

    ParB1
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    pBAD/tetO

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCM189-ParB1::mCherry

    Plasmid

    #87253

    Purpose

    Expresses ParB1-mCherry in yeast. Also known as pOR1-mch

    Depositor

    Kerstin Bystricky

    Article

    Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

    Insert

    ParB1
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    pBAD/tetO

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Pdest-EF1α-EGFP-32bp barcode polyA library

    Pooled Library

    #211610

    Purpose

    Barcoded retrovirus plasmid library for capturing the transcriptome and viral barcode sequences of individual cells to infer their identities and lineage information.

    Depositor

    Yuejun Chen

    Article

    You et al Cell Stem Cell. 2023 Apr 6;30(4):473-487

    Species

    Synthetic

    Use

    Retroviral

    Available since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only