We narrowed to 11,732 results for: Emb;
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Plasmid#162949PurposeGateway attB5r/attB1r entry clone containing divergent AcMNPV polyhedrin and p10 promoters, for use in construction of polycistronic baculovirus expression vectorsDepositorInsertdual baculovirus promoter cassette (AcMNPV p10 and polyhedrin)
UseSynthetic BiologyAvailable SinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
C239-E02: attB2r-IRES-ffLuc2-eGFP-pA-attB3
Plasmid#162918PurposeGateway attB2r/attB3 entry clone for generation of polycistronic ffLuc2-eGFP reporter using an internal ribosome entry sequence (IRES)DepositorInsertfirefly luciferase/green fluorescent protein fusion with upstream IRES sequence
UseSynthetic BiologyAvailable SinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYP-SELF
Plasmid#158770PurposeExpression of synaptophysin 1 variant with self-cleaving c-terminal tail.DepositorInsertSynaptophysin 1-NS3/4a (Syt1 Mouse)
UseLentiviralMutationFlexible linkers, ADVVCC/QMSY cleavage siteAvailable SinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnn1b
Plasmid#164957PurposeZebrafish kcnn1b gene CDSDepositorInsertkcnn1b (kcnn1b Zebrafish)
UsePcr cloning vectorAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDF11-SFPQ(369-598)
Plasmid#135438PurposeExpresses SFPQ 369-598 with TEV-cleavable hexahistidine tagDepositorInsertsplicing factor proline and glutamine rich (SFPQ Human)
TagsHexahistidineExpressionBacterialPromoterT7Available SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMiNGo11-3
Plasmid#126058PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cellDepositorInsertsmitochondria Rieske iron sulphur protein (1-100 aa)
ccdB cassette (BsaI)
GFP11x3 (5 aa linker)
UseBinaryTagsGFP1-10ExpressionPlantAvailable SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMiNGo11-7
Plasmid#126057PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cellDepositorInsertsmitochondria Rieske iron sulphur protein (1-100 aa)
ccdB cassette (BsaI)
GFP11x7 (5 aa linker)
UseBinaryTagsGFP1-10ExpressionPlantAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MBP1-E322TAG-W340A.pcDNA3-k
Plasmid#127404PurposeExpresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site.DepositorInsertMaltose Binding Protein
TagsFLAGExpressionMammalianMutationAmino acid mutations: E322 to Amber codon and W34…PromoterCMVAvailable SinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
SKM2 X057_pECIA14
Plasmid#114914PurposePrey vector SKM2 X057_pECIA14 should be used with bait vector SKM2 X057_pECIA2.DepositorInsertAT5G56040 (AT5G56040 Mustard Weed)
ExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
SKM1 nA12_pECIA14
Plasmid#114822PurposePrey vector SKM1 nA12_pECIA14 should be used with bait vector SKM1 nA12_pECIA2.DepositorInsertAT2G25790 (AT2G25790 Mustard Weed)
ExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
SERK2 P5_pECIA14
Plasmid#114768PurposePrey vector SERK2 P5_pECIA14 should be used with bait vector SERK2 P5_pECIA2.DepositorAvailable SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIDS-LSD1-C
Plasmid#109160PurposeEncodes a human C-terminal LSD1 fragment to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman C-terminal LSD1, sequence-optimized for insect cells (KDM1A Human)
ExpressionInsectPromoterp10Available SinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 p37 SHPmut
Plasmid#113503PurposeBacterial expression of GST-tagged p37 that is p97 binding deficient due to mutations in the SHP boxDepositorInsertp37 (Ubxn2b Mouse)
TagsGST-PreScission protease siteExpressionBacterialMutationF215A/E218A/QKL220-222AAAAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-puro-eGFP-HEC1-9A
Plasmid#114023PurposeInducible expression of eGFP-tagged HEC1-9A in FLPin Trex cell linesDepositorAvailable SinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-A8REH9
Plasmid#103084PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertA8REH9(Cas9 coding gene from Eubacterium dolichum DSM 3991)
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-K1LM04
Plasmid#103119PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertK1LM04(Cas9 coding gene from Bergeyella zoohelcum ATCC 43767)
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-Q4A5I2
Plasmid#103125PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertQ4A5I2(Cas9 coding gene from Mycoplasma synoviae (strain 53))
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-Q5X8T6
Plasmid#103127PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertQ5X8T6(Cas9 coding gene from Legionella pneumophila (strain Paris))
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-E3ELL7
Plasmid#103099PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertE3ELL7(Cas9 coding gene from Bifidobacterium bifidum (strain S17))
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-E7MR72
Plasmid#103105PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertE7MR72(Cas9 coding gene from Solobacterium moorei F0204)
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-F3QMY7
Plasmid#103107PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertF3QMY7(Cas9 coding gene from Parasutterella excrementihominis YIT 11859)
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-G2ZYP2
Plasmid#103110PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertG2ZYP2(Cas9 coding gene from Ralstonia syzygii R24)
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-G9QLF2
Plasmid#103111PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertG9QLF2(Cas9 coding gene from Bacillus smithii 7_3_47FAA)
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-G9WGU4
Plasmid#103112PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertG9WGU4(Cas9 coding gene from Oenococcus kitaharae DSM 17330)
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-B9M9X8
Plasmid#103072PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertB9M9X8(Cas9 coding gene from Acidovorax ebreus (strain TPSY))
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-D3NT09
Plasmid#103074PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertD3NT09(Cas9 coding gene from Azospirillum sp. (strain B510))
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-E3CY56
Plasmid#103075PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertE3CY56(Cas9 coding gene from Aminomonas paucivorans DSM 12260)
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-J9E534
Plasmid#103078PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertJ9E534(Cas9 coding gene from Alicyclobacillus hesperidum URH17-3-68)
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-K2FTY8
Plasmid#103079PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertK2FTY8(Cas9 coding gene from Alcanivorax pacificus W11-5)
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-B1UZL4
Plasmid#103085PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertB1UZL4(Cas9 coding gene from Clostridium perfringens D str. JGS1721)
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-HRKI(Haunt P-Inr2-cDNA KI)
Plasmid#92163PurposeFor lncRNA CAG promoter homologue recombination mediated knockinDepositorInsertHaunt CAG KI homology arms
UseOtherAvailable SinceFeb. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Kind2 S181E
Plasmid#105312PurposeTo study altered downstream functions of phospholylation-site mimetic kindlin2DepositorAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Kind2 S181A
Plasmid#105307PurposeTo study altered downstream functions of phospholylation-site null kindlin2DepositorAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
6xHis-TEV-SMO-1(GG)
Plasmid#87054PurposeExpresses 6xHis-tagged smo-1(GG), with a TEV protease cleavage site between the tag and the ORFDepositorInsertsmo-1 (smo-1 Nematode)
Tags6xHis-TEVExpressionBacterialMutationThe last aa was removed to render processed SMO-1…PromoterT7Available SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
FCIV1-GR SS287AA/S155A
Plasmid#72700PurposeLentiviral expression of GR-2A phosphodeficient mutant (2nd generation)DepositorInsertGR (Nr3c1 Rat)
UseLentiviralTagsIRES-VenusExpressionMammalianMutationSS287AA/S155APromoterubiquitinAvailable SinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pLacIBD::SDRX (GB0673)
Plasmid#68183PurposeProvides the LacI Binding Domain fused to SRDX Transcriptional Repressor as a level 0 GoldenBraid partDepositorInsertLacI Binding Domain fused to SRDX Transcriptional Repressor
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
NET4 pEGFP-N2 (1201)
Plasmid#62041Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable SinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM3-K83A
Plasmid#58401PurposeExpresses murine IFITM3-K83A with an N-terminal HA tag in mammalian cellsDepositorInsertIFITM3 (Ifitm3 Mouse)
TagsHAExpressionMammalianMutationLysine 83 to AlaninePromoterCMV IEAvailable SinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM3-K24A
Plasmid#58400PurposeExpresses murine IFITM3-K24A with an N-terminal HA tag in mammalian cellsDepositorInsertIFITM3 (Ifitm3 Mouse)
TagsHAExpressionMammalianMutationLysine 24 to AlaninePromoterCMV IEAvailable SinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
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SL539
Plasmid#49948PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding siteDepositorAvailable SinceJan. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA3
Plasmid#46790PurposeTo express ATPase inactive Fun30 in budding yeastDepositorInsertFUN30 (FUN30 Budding Yeast)
TagsV5ExpressionYeastMutationATP-binding site mutated replaced lysin 603 by ar…PromoterPGAL1Available SinceAug. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1 hSlp4-a V21A
Plasmid#40064DepositorInserthSlp4-a V21A (SYTL4 Human)
TagsGSTExpressionBacterialMutationValine 21 to AlaninePromoterTacAvailable SinceOct. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCaSpeR-4 porcupine (genomic DNA)
Plasmid#37376DepositorInsertporcupine (por Fly)
ExpressionInsectAvailable SinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEMS1097
Plasmid#29028PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorInsertPle201 (SLC6A5 Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-Cre-NLSAvailable SinceJan. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEMS1408
Plasmid#29205PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorAvailable SinceNov. 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEMS1673
Plasmid#29290PurposePleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).DepositorInsertPle201 (SLC6A5 Human)
UsePleiades promoter project [sic, pleaides plieades]TagsIntron-LacZ-NLSAvailable SinceJune 10, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDNA3 Flag Bmf (S74D)
Plasmid#24259DepositorInsertBmf (S87D) (BMF Human)
TagsFlagExpressionMammalianMutationchanged Serine 74 to Aspartic AcidAvailable SinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only