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We narrowed to 6,891 results for: GFP expression plasmids

Showing: 6651 - 6700 of 6891 results
  1. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-8

    Plasmid
    #129048
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA8 targeting human TSDR
    Depositor
    Insert
    dCas9-hudTET1CD, SgRNA8 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-1

    Plasmid
    #129041
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA1 targeting human TSDR
    Depositor
    Insert
    dCas9-hudTET1CD, SgRNA1 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-7

    Plasmid
    #129047
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA7 targeting human TSDR
    Depositor
    Insert
    dCas9-hudTET1CD, SgRNA7 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-9

    Plasmid
    #129049
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA9 targeting human TSDR
    Depositor
    Insert
    dCas9-hudTET1CD, SgRNA9 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-10

    Plasmid
    #129050
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA10 targeting human TSDR
    Depositor
    Insert
    dCas9-hudTET1CD, SgRNA10 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-11

    Plasmid
    #129051
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA11 targeting human TSDR
    Depositor
    Insert
    dCas9-hudTET1CD, SgRNA11 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  7. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-12

    Plasmid
    #129052
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA12 targeting human TSDR
    Depositor
    Insert
    dCas9-hudTET1CD, SgRNA12 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  8. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-3

    Plasmid
    #129043
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA3 targeting human TSDR
    Depositor
    Insert
    dCas9-hudTET1CD, SgRNA3 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  9. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-4

    Plasmid
    #129044
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA4 targeting human TSDR
    Depositor
    Insert
    dCas9-hudTET1CD, SgRNA4 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  10. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-5

    Plasmid
    #129045
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA5 targeting human TSDR
    Depositor
    Insert
    dCas9-hudTET1CD, SgRNA5 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  11. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-6

    Plasmid
    #129046
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA6 targeting human TSDR
    Depositor
    Insert
    dCas9-hudTET1CD, SgRNA6 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  12. pLV-EF1A-CD19.28H.28TM.BB.3z

    Plasmid
    #200679
    Purpose
    Modular CD28hinge-CD28transmembrane-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production
    Depositor
    Insert
    FMC63-CD28HD-CD28TM-41BB-CD3z-P2A-EGFP (CD28, CD247, TNFRSF9 Human)
    Use
    Lentiviral
    Tags
    MycTag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a-short
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
  13. pZJQIBEBT003

    Plasmid
    #140053
    Purpose
    The pZJQIBEBT003 plasmid contains the coding sequence of eMutS-L157C-G233C gene which has the ability of error removal in the gene synthesis process.
    Depositor
    Insert
    eMutS-L157C-G233C-CBM-EGFP
    Use
    Tags
    His-Tag
    Expression
    Bacterial
    Mutation
    L157C, G233C
    Promoter
    T7
    Available since
    June 3, 2020
    Availability
    Academic Institutions and Nonprofits only
  14. pLV-EF1A-CD19.28H.28TM.BB.28.3z

    Plasmid
    #200681
    Purpose
    Modular CD28hinge-CD28transmembrane-41BB-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production
    Depositor
    Insert
    FMC63-CD28HD-CD28TM-41BB-CD28-CD3z-P2A-EGFP (CD28, CD247, TNFRSF9 Human)
    Use
    Lentiviral
    Tags
    MycTag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a-short
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
  15. pSUC2::GNSI (NrsR)

    Plasmid
    #121537
    Purpose
    This plasmid contains a CYC1pr driven GNSI reporter and flanking homology to SUC2. Digestion with NotI, SacI, and EcoRV, allows integration at the SUC2 locus.
    Depositor
    Inserts
    CYC1 Promoter (CYC1 Budding Yeast)
    Nyv1 Cytosolic Domain (aa 6-230)
    Snc1 Transmembrane Domain (TMD)
    SUC2 CDS
    SUC2 terminator
    NrsR
    Use
    Tags
    mGFP5
    Expression
    Yeast
    Mutation
    M109I (Naturally occurring SNP) and The first 21 …
    Promoter
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
  16. SunTagCACTA1g2-22aa-TET1cd

    Plasmid
    #106437
    Purpose
    SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the CACTA1 promoter
    Depositor
    Insert
    CACTA1gRNA2_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    March 16, 2018
    Availability
    Academic Institutions and Nonprofits only
  17. pAR100

    Plasmid
    #180377
    Purpose
    pAR100 allows cloning of TCRα and TCRβ genes using restriction enzymes (AflII, ApaI, and SacII).
    Depositor
    Inserts
    NFAT-Luciferase
    hCD8A
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    hEF1-HTLV and minimal promoter and an NFAT respon…
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
  18. NESRA-DEVD-BNLS

    Plasmid
    #50849
    Purpose
    Expresses a tandem red ddFP heterodimer in mammalian cells, construct 1 for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid GANLS.
    Depositor
    Insert
    ddRFP A and ddRFP B
    Use
    Tags
    NES sequence LQKKLEELELDE placed after ddGFP A an…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
  19. XanthoMoClo Xanthobacter Genetic Toolkit

    Plasmid Kit
    #1000000259
    Purpose
    Use this modular cloning kit to assemble plasmids for expression in Xanthobacter and Roseixanthobacter.
    Depositor
    Available since
    June 16, 2025
    Availability
    Academic Institutions and Nonprofits only
  20. pAAV.cTNT.iCre

    Plasmid
    #69916
    Purpose
    Used to package AAV9:cTNT.iCre, which specifically transduce cardiomyocytes and express Cre recombinase. Only works in vivo.
    Depositor
    Has Service
    AAV9
    Insert
    improved cre (iCre) recombinase and tdTomato
    Use
    AAV
    Tags
    The iCre coding frame and tdTomato coding frame i…
    Expression
    Mutation
    Promoter
    Chicken cardiac troponin T
    Available since
    Dec. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
  21. pXW117As(RS-E11-RinA)2

    Plasmid
    #123146
    Purpose
    Arsenic sensor with three-layered amplifier cascade. Need to work with plasmid pXWPrinA-gfp2. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-t
    Depositor
    Insert
    J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-t
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J117, ParsR, PhrpLE, Pecf11
    Available since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
  22. aCat DeltaTS (delta570 to 670 aa in aCat TS WT)

    Plasmid
    #101300
    Purpose
    FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin. See Depositor Comments for plasmid construction.
    Depositor
    Insert
    alpha-Catenin
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Contains amino acids 1-509, 670-698, and 699-906 …
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  23. pXW109Hg(RS-RinA-E11)2

    Plasmid
    #123151
    Purpose
    Mercury sensor with three-layered amplifier cascade. Need to work with plasmid pXWP11-gfp2. pSB4A3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t
    Depositor
    Insert
    J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J109, PmerT, PhrpLE, PrinA
    Available since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
  24. ss-ED-mCherry-GPI

    Plasmid
    #163680
    Purpose
    Exprsses the GPI-anchored luminal domain of ST6GAL1 with a mCherry tag in mammalian cells
    Depositor
    Insert
    The luminal domain of ST6 beta-galactoside alpha-2,6-sialyltransferase 1 with N-terminally tagged signal sequences (ST6GAL1 Human)
    Use
    Tags
    GPI anchor, mCherry, and signal sequences
    Expression
    Mammalian
    Mutation
    This chimera contains the ecto-domain of ST. Its …
    Promoter
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
  25. SunTagng22aa

    Plasmid
    #106438
    Purpose
    Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome.
    Depositor
    Insert
    NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
  26. SunTagng14aa

    Plasmid
    #106439
    Purpose
    Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome.
    Depositor
    Insert
    NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN414aa_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
  27. SunTag-FWAgRNA-4-22aa-TET1cd

    Plasmid
    #106435
    Purpose
    SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoter
    Depositor
    Insert
    gRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    March 16, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 6651 - 6700 of 6891 results