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Showing: 6801 - 6850 of 11732 results

  1. pUC19-Q5L8F8

    Plasmid
    #103126
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    Q5L8F8(Cas9 coding gene from Bacteroides fragilis (strain ATCC 25285 / NCTC 9343))
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available Since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103126-plasmid-map-sequence-id-201889

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  2. pUC19-E6WZS9

    Plasmid
    #103103
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    E6WZS9(Cas9 coding gene from Nitratifractor salsuginis (strain DSM 16511 / JCM 12458 / E9I37-1))
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available Since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103103-plasmid-map-sequence-id-201812

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  3. pUC19-F4GDP9

    Plasmid
    #103077
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    F4GDP9(Cas9 coding gene from Alicycliphilus denitrificans (strain DSM 14773 / CIP 107495 / K601))
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available Since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103077-plasmid-map-sequence-id-201717

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  4. pUC19-A8LN05

    Plasmid
    #103083
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    A8LN05(Cas9 coding gene from Dinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12))
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available Since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103083-plasmid-map-sequence-id-201724

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  15. TTMP-His-bio

    Plasmid
    #52335
    Purpose
    Expresses full-length TPA-induced transmembrane protein ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    TTMP (C3orf52 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52335-plasmid-map-sequence-id-81056

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  19. pChlr-1SR

    Plasmid
    #52142
    Purpose
    Encodes module 1 with amino acids 12S-16R for recognition of nt 8C
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 25-60 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 16Q to 16R in Module 1
    Available Since
    May 5, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. pChlr-1NQ

    Plasmid
    #52141
    Purpose
    Encodes module 1 with amino acids 12N-16Q for recognition of nt 8U
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 25-60 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12S to 12N in Module 1
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  21. pChlr-2CQ

    Plasmid
    #52143
    Purpose
    Encodes module 2 with amino acids 12C-16Q for recognition of nt 7A
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 61-96 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12C in Module 2
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  22. pChlr-4SYE

    Plasmid
    #52156
    Purpose
    Encodes module 4 with amino acids 12S-13Y-16E for recognition of nt 5G
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    D43951 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S, 13H to 13Y, and 16Q to 16E in…
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  29. FSW CHIP-Myc

    Plasmid
    #246724
    Purpose
    Neuron-specific expression of human CHIP wildtype, and a myc-tag on its C-terminus
    Depositor
    Yihong Ye
    Article
    Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038
    Insert
    CHIP (Carboxy terminus of Hsc70-interacting protein) (STUB1 Human)
    Use
    Lentiviral
    Tags
    Myc
    Expression
    Mammalian
    Promoter
    Synapsin
    Available Since
    March 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    246724-plasmid-map-sequence-id-501657

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  36. Lyn11-BirA*-HA

    Plasmid
    #214474
    Purpose
    Lentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with an N-terminal Lyn11 plasma membrane localization sequence and C-terminal HA tag.
    Depositor
    Natalie Ahn
    Article
    Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.
    Insert
    BirA(R118G)
    Use
    Gateway Cloning and Lentiviral
    Tags
    HA tag and Lyn11 (Plasma Membrane Localization Se…
    Expression
    Mammalian
    Mutation
    R118G
    Promoter
    EF-1a
    Available Since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214474-plasmid-map-sequence-id-425600

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  38. nCre-ST-NLS-P2A-NLS-pSC-cCre

    Plasmid
    #207638
    Purpose
    A plasmid encoding N-terminal Cre fused to SpyTag (ST) and photocaged SpyCatcher (pSC) fused to C-terminal Cre with a nuclear localization signal (NLS) sequence
    Depositor
    Cole DeForest
    Article
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Insert
    nCre-SpyTag-NLS-P2A-NLS-photocaged SpyCatcher-cCre
    Expression
    Mammalian
    Mutation
    Amber stop codon at SpyCatcher’s critical lysine
    Promoter
    CMV
    Available Since
    Oct. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    207638-plasmid-map-sequence-id-409880

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  40. pCDNA-CRY2-mCh-superPLDx50-P2A-CIBN-CAAX

    Plasmid
    #188993
    Purpose
    A plasmid for dual expression of CRY2-mCh-superPLDx50 (an engineered PLD mutant with 50 times higher activity than the wild-type) and plasma membrane-tagged CIBN
    Depositor
    Jeremy Baskin
    Article
    Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415
    Insert
    PLD
    Tags
    CAAX, CIBN, CRY2, P2A, and mCherry
    Expression
    Mammalian
    Mutation
    D201G, A258T, K327R, G328S, G381V, G429D, T450A
    Promoter
    CMV
    Available Since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    188993-plasmid-map-sequence-id-371646

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  43. trp1D-proLYS2-GFP-PKCdelta(C1a+C1b)-NAT

    Plasmid
    #184774
    Purpose
    Marker for DAG. Yeast expression of mouse PKCdelta under LSY2 promoter. Uses antibiotic resistance marker natMX6. Replaces endogenous TRP1 upon genome integration, leading to trp1D.
    Depositor
    Zhiping Xie
    Article
    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10
    Insert
    proLYS2-GFP-PKCdelta(C1a+C1b) (Prkcd Mouse, Budding Yeast)
    Tags
    EGFP
    Expression
    Yeast
    Available Since
    Feb. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    184774-plasmid-map-sequence-id-382498

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  48. EGFP-uTEV1-MAVS-mTA

    Plasmid
    #170999
    Purpose
    HiLITR protease with MAVS [R537A] C-terminal tmd targeting information (Mitochondria and ER insertion)
    Depositor
    Alice Ting
    Article
    Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:
    Insert
    EGFP-uTEV1-MAVS(tmd)[R537A]
    Use
    Lentiviral and Synthetic Biology
    Expression
    Mammalian
    Mutation
    MAVS(tmd)[R537A]
    Promoter
    pTRE-tight (TetOn)
    Available Since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    170999-plasmid-map-sequence-id-335667

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  49. EGFP-uTEV1-MAVS-mTA4

    Plasmid
    #171002
    Purpose
    HiLITR protease with MAVS [Y536delinsFIVLI] C-terminal tmd targeting information (Mitochondria and ER insertion)
    Depositor
    Alice Ting
    Article
    Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:
    Insert
    EGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI]
    Use
    Lentiviral and Synthetic Biology
    Expression
    Mammalian
    Mutation
    MAVS(tmd)[Y536delinsFIVLI]
    Promoter
    pTRE-tight (TetOn)
    Available Since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    171002-plasmid-map-sequence-id-335829

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  50. pMP108 Nanog-Venus-2a-mCherry

    Plasmid
    #159743
    Purpose
    Nanog targeting vector to insert fluorescent reporter of protein and gene expression levels from endogenous locus in mouse embryonic stem cells. Please see depositor comments for more detail.
    Depositor
    Sergi Regot
    Article
    Pokrass et al Dev Cell. 2020 Nov 9;55(3):328-340.e5. d
    Insert
    Nanog homeobox (Nanog Mouse)
    Use
    Mouse Targeting
    Tags
    Venus-2a-mCherry
    Available Since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    159743-plasmid-map-sequence-id-330621

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Showing: 6801 - 6850 of 11732 results