We narrowed to 13,452 results for: Mpl;

1. pFl Strep-sumo-TEV-hAgo2

   Plasmid

   #136235

   Purpose

   Expresses human Argonaute-2 in insect cells

   Depositor

   Leemor Joshua-Tor

   Article

   Elkayam et al Cell. 2012 Jul 6;150(1):100-10. doi: 10.

   Insert

   human Argonaute-2 (AGO2 Human)
   

   Tags

   Strep Sumo

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   May 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.3−3xFLAG−AGO2(D669A)

   Plasmid

   #220461

   Purpose

   Express catalytically-dead mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

   Depositor

   David Bartel

   Article

   Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

   Insert

   Human AGO2 (AGO2 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Aspartate 669 to alanine

   Promoter

   CMV

   Available Since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. GFP-itis pBE-t

   Plasmid

   #195342

   Purpose

   Base editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed gRNA targeting EGFP A110V

   Depositor

   Alexis Komor

   Article

   Vasquez et al CRISPR J. 2023 Apr 20. doi: 10.1089/cris

   Inserts

   ecTadA(8e)-SpCas9-NG
   gRNA ctctacgcgggtcttgtagt
   

   Use

   CRISPR

   Mutation

   SpCas9n-NG: D10A, L1111R, D1135V, G1218R, E1219F,…

   Promoter

   Lac and ProC

   Available Since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCDNA4.TO-ORF50-2xCSTREP

   Plasmid

   #136211

   Purpose

   Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF50

   Depositor

   Britt Glaunsinger

   Article

   Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

   Insert

   ORF50
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEGFP-n1-AICD

   Plasmid

   #69925

   Purpose

   mammalian expression of human AICD fused to EGFP

   Depositor

   Thomas Wassmer, Zita Balklava

   Article

   Currinn et al Cell Mol Life Sci. 2015 Jul 28.

   Insert

   AICD (APP Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   47 aa long

   Promoter

   CMV

   Available Since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pENTR221-TCF7

   Plasmid

   #79498

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Human TCF7 CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   TCF7 (TCF7 Human)
   

   Use

   Multisite gateway entry clone

   Promoter

   no Promoter

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDONR_P2R-P3_R2-miniTurbo-NES-mVenus-STOP-L3

   Plasmid

   #127355

   Purpose

   gateway entry vector for making C-terminal miniTurbo-mVenus fusion with non-nuclear proteins

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, NES, V5, and mVenus

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   no promoter

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDONR_P2R-P3_R2-miniTurbo-mVenus-STOP-L3

   Plasmid

   #127356

   Purpose

   gateway entry vector for making C-terminal miniTurbo-mVenus fusion with nuclear and non-nuclear proteins

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, V5, and mVenus

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   no promoter

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDNA4.TO-ORF73-2xCSTREP

   Plasmid

   #136232

   Purpose

   Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF73

   Depositor

   Britt Glaunsinger

   Article

   Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

   Insert

   ORF73
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA -CLCN3-3xFlag

    Plasmid

    #225506

    Purpose

    CLCN3 expression vector

    Depositor

    Wade Harper

    Article

    Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

    Insert

    CLCN3 (CLCN3 Human)
    

    Tags

    3xFlag

    Expression

    Mammalian

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GFP-itis pBE-nt

    Plasmid

    #195343

    Purpose

    Base editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed nonsense non-targeting gRNA

    Depositor

    Alexis Komor

    Article

    Vasquez et al CRISPR J. 2023 Apr 20. doi: 10.1089/cris

    Inserts

    ecTadA(8e)-SpCas9-NG
    gRNA gtgcacgacgccgtatgcga
    

    Use

    CRISPR

    Mutation

    SpCas9n-NG: D10A, L1111R, D1135V, G1218R, E1219F,…

    Promoter

    Lac and ProC

    Available Since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. qTAG-C-miRFP670nano3-Blast-H2BC11

    Plasmid

    #207762

    Purpose

    Donor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H2BC11 Homology Arms flanking a miRFP670nano3-Blast Cassette (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 29, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. p3xFLAG-CMV10-hAtg13

    Plasmid

    #22872

    Depositor

    Noboru Mizushima

    Article

    Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

    Insert

    autophagy-related genes 13 (ATG13 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Feb. 25, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pTY-EF1a-wild type mKdm2b-Flag

    Plasmid

    #61739

    Purpose

    Express wild-type mouse histone demethylase Kdm2b

    Depositor

    Yi Zhang

    Article

    He et al Nat Cell Biol. 2013 Apr;15(4):373-84. do

    Insert

    Kdm2b (Kdm2b Mouse)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pIVT/CamKII gamma CA

    Plasmid

    #122142

    Purpose

    For making constitutively active CamKII gamma RNA

    Depositor

    Carmen Williams

    Article

    Miao et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

    Insert

    constitutively active CamKII gamma (Camk2g Mouse)
    

    Use

    For in vitro transcription

    Mutation

    First 291 amino acid residues of CaMKII gamma

    Promoter

    T7

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Cav2.2 pcDNA3

    Plasmid

    #206072

    Purpose

    expression of rabbit Cav2.2 calcium channel, the 3'UTR is deleted, this enhances the expression

    Depositor

    Annette Dolphin

    Article

    Page et al J Neurosci. 2004 Jun 9;24(23):5400-9. do

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Cox7c-pHLuorin-sems

    Plasmid

    #107766

    Purpose

    labeling of mitochondrial cytochrome c oxidase, pH measurements

    Depositor

    Karin Busch

    Article

    Rieger et al Sci Rep. 2017 Apr 6;7:46055. doi: 10.103

    Insert

    Subunit Cox7c fused to pHluorin (COX7C Human)
    

    Tags

    pHluorin

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. NSP4-WT-Flag

    Plasmid

    #210339

    Purpose

    Expresses SARS-CoV-2 NSP4 fused with Flag in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    SARS-CoV-2 NSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFRT/TO/FLAG/HA-DEST TNRC6B

    Plasmid

    #19884

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    trinucleotide repeat containing 6B (TNRC6B Human)
    

    Tags

    FLAG/HA

    Expression

    Mammalian

    Available Since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. plenti-UBC-HA-TMEM230

    Plasmid

    #225516

    Purpose

    Lentiviral TMEM230 expression vector

    Depositor

    Wade Harper

    Article

    Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

    Insert

    TMEM230 (TMEM230 Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAPM-D4 miR30-FAM208A ts2

    Plasmid

    #115872

    Purpose

    FAM208a knockdown

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    FAM208a shRNA (C3orf63 Human)
    

    Use

    Lentiviral

    Promoter

    SFFV

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA 3.3 hFZD4

    Plasmid

    #115908

    Purpose

    Expresses human FZD4 in mammalian cells.

    Depositor

    Harald Junge

    Article

    Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

    Insert

    FZD4 (FZD4 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Rab8WT(Flag) in pcDNA3.1neo

    Plasmid

    #46783

    Purpose

    expression of WT Rab8a in mammalian cells

    Depositor

    Terry Hébert

    Article

    Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

    Insert

    RAB8A (RAB8A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

24. YES1 Y537F

    Plasmid

    #51299

    Purpose

    Expression of YES1 Y537F

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    YES1 (YES1 Human)
    

    Use

    Lentiviral

    Mutation

    Y537F

    Promoter

    CMV

    Available Since

    Feb. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCDNA4.TO-ORF64-2xNSTREP

    Plasmid

    #136224

    Purpose

    Expresses N-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF64

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF64
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCDNA4.TO-ORF26-2xCSTREP

    Plasmid

    #136186

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF26

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF26
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLV-Hygro-EF1A-OB-Linker-eGFP

    Plasmid

    #176068

    Purpose

    EGFP fused to the C-terminus of an OB domain & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    OB
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. HaloTag-WDR5

    Plasmid

    #200880

    Purpose

    Mammalian expression of human WDR5 with N-terminal HaloTag fusion

    Depositor

    Stefan Knapp

    Article

    Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

    Insert

    HaloTag-WDR5 (WDR5 Human)
    

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJZC116

    Plasmid

    #62344

    Purpose

    sgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells, marked by BFP

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA + 2x MS2 (wt+f6) binding module
    MCP-VP64
    

    Use

    Lentiviral

    Tags

    VP64

    Expression

    Mammalian

    Mutation

    Targets CXCR4 , sequence: GCAGACGCGAGGAAGGAGGGCGC

    Promoter

    CMV and U6

    Available Since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pNS20-SpCas9-SNAP

    Plasmid

    #113717

    Purpose

    Bacterial vector for expression of Snap-tagged Streptococcus pyogenes Cas9

    Depositor

    Gerald Schwank

    Article

    Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA-2xNLS-SNAP-NLS and His6-MBP-TEV

    Expression

    Bacterial

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. SNAP-BAF

    Plasmid

    #227443

    Purpose

    Mammalian expression of SNAP-BAF

    Depositor

    David Pellman

    Article

    Umbreit et al Science. 2020 Apr 17;368(6488):eaba0712.

    Insert

    Barrier-to-autointegration factor (BANF1 Human)
    

    Use

    Lentiviral

    Tags

    SNAP

    Expression

    Mammalian

    Available Since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Flag WDR24 pRK5

    Plasmid

    #46334

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    WDR24 (WDR24 Human)
    

    Tags

    FLag

    Expression

    Mammalian

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLV-Hygro-EF1A-LivePARBackbone

    Plasmid

    #176526

    Purpose

    Expression vector with a BamHI site in-frame with a Gly-Ser linker fused to EGFP; serves as the backbone for PAR binding domain incorporation for LivePAR

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLKO.1 p18 shRNA

    Plasmid

    #26631

    Depositor

    David Sabatini

    Article

    Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

    Insert

    p18 shRNA (LAMTOR1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

35. pET28-MHL: nsp16_SARS-CoV-2

    Plasmid

    #167850

    Purpose

    Bacterial expression of the SARS-CoV-2 protein nsp16

    Depositor

    Cheryl Arrowsmith

    Article

    Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

    Insert

    nsp16
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pGEX-6P-1-INTS3-FL

    Plasmid

    #128415

    Purpose

    Express INTS3 full-length in E. coli with a GST tag (N-terminal)

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

    Insert

    INTS3 (INTS3 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    full-length

    Promoter

    tac promoter

    Available Since

    Aug. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. qTAG-C-moxGFP-Zeo-H2BC11

    Plasmid

    #207759

    Purpose

    Donor template for moxGFP-2A-Zeo insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H2BC11 Homology Arms flanking a moxGFP-Zeo Cassette (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 17, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pENTR221-YAP1

    Plasmid

    #79503

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human YAP1 CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Multisite gateway entry clone

    Tags

    FLAG

    Promoter

    no Promoter

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCDNA4.TO-ORF22-2xCSTREP

    Plasmid

    #136182

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF22

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF22
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCDNA4.TO-ORF47-2xCSTREP

    Plasmid

    #136208

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF47

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF47
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pBrain-GFP-TACC3KDP-shTACC3

    Plasmid

    #59356

    Purpose

    Knocks down endogenous TACC3 and re-expresses knockdown-proof (RNAi-resistant) GFP-tagged human TACC3.

    Depositor

    Stephen Royle

    Article

    Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

    Insert

    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    

    Use

    RNAi

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Silent mutations in amino acids 30-33 to confer s…

    Promoter

    CMV

    Available Since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pSpCas9_U6_sgRNA_Mettl3C

    Plasmid

    #165420

    Purpose

    sgRNA construct for targeting Mettl3 C-terminus

    Depositor

    Neil Brockdorff

    Article

    Wei et al Genome Res. 2021 Aug;31(8):1395-1408. do

    Insert

    PspCas9 gRNA targeting Mettl3 C-terminus (Mettl3 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. L2_lacZgRNA-Cas9-CsA

    Plasmid

    #136138

    Purpose

    Plasmid to accept gRNA target with SapI, lacZ blue-white screening. Contains Cas9 and HygR.

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-35Sx2:HygR p5-MpU6:lacZgRNA p5-MpEF1a:Cas9-NLS
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. EGFP-Rab8a-QL

    Plasmid

    #86076

    Purpose

    A mammalian expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus EGFP.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    hRab8A (RAB8A Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed glutamine 67 to leucine

    Promoter

    CMV

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. PCMV-intron myc Rab6WT

    Plasmid

    #46781

    Purpose

    expression of WT Rab6a in mammalian cells

    Depositor

    Terry Hébert

    Article

    Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

    Insert

    Rab6 (RAB6A Human)
    

    Tags

    myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pLV-Hygro-EF1A-FHA-Linker-eGFP

    Plasmid

    #176065

    Purpose

    EGFP fused to the C-terminus of a FHA domain & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    FHA
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pSF3-Flag-Cbir-Ago2_Myc-Nbir-GFP

    Plasmid

    #90015

    Purpose

    Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-GFP

    Depositor

    Julien Béthune

    Article

    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

    Inserts

    CBirA*-Ago2
    NBirA*-GFP
    

    Use

    Retroviral; Flp/frt

    Tags

    FLAG and Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pCAT-YFP-TetR

    Plasmid

    #59018

    Purpose

    Expresses a fusion of yellow fluorescent protein (YFP) to the N-terminus of the Escherichia coli Tn10 tet repressor (TetR) driven by the alpha tubulin promoter for use in T. gondii

    Depositor

    David Sibley

    Article

    Etheridge et al Cell Host Microbe. 2014 May 14;15(5):537

    Insert

    YFP-TetR
    

    Use

    Toxoplasma expression

    Promoter

    alpha-tubulin

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pAAV-SynI-CreOn-FlpOff-mNaChBacMUT-P2A-EGFP

    Plasmid

    #176281

    Purpose

    Viral vector for co-expression of non-functional NaChBac and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

    Depositor

    Martin Riccomagno

    Article

    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    mNaChBacMUT-P2A-EGFP
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Mutation

    E191K

    Promoter

    human Synapsin I

    Available Since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pCAG-hPPP3CC-FLAG

    Plasmid

    #179162

    Purpose

    Expression vector of human protein phosphatase 3, catalytic subunit, gamma isoform (PPP3CC) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Science. 2015 Oct 23;350(6259):442-5. do

    Insert

    protein phosphatase 3, catalytic subunit, gamma isoform (PPP3CC Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge