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We narrowed to 14,561 results for: cas9 genes

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Showing: 2721 - 2740 of 14561 results

  1. pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196074
    Purpose
    (Empty Backbone) Constitutive CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196074-plasmid-map-sequence-id-389001

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  2. pAAV-synmiR-L-mRuby-Pro-dCas9-EGFP-4×17

    Plasmid
    #218268
    Purpose
    Expresses dCas9-EGFP in mammalian cells regulated by a synthetic microRNA-based dosage compensation circuit
    Depositor
    Michael Elowitz
    Insert
    synPolyA-synmiRL-mRuby-EF1a-CMVe_MeP-dCas9-EGFP-MREL_4×17
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Dec. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    218268-plasmid-map-sequence-id-494321

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  3. pAAV-synmiR-L-mRuby-Pro-dCas9-EGFP-4×19

    Plasmid
    #218270
    Purpose
    Expresses dCas9-EGFP in mammalian cells regulated by a synthetic microRNA-based dosage compensation circuit
    Depositor
    Michael Elowitz
    Insert
    synPolyA-synmiRL-mRuby-EF1a-CMVe_MeP-dCas9-EGFP-MREL_4×19
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Dec. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    218270-plasmid-map-sequence-id-494320

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  10. pUC19-U6-BsmBI_cassette-Nme/Nme2Cas9-sgRNA (KAC32)

    Plasmid
    #133794
    Purpose
    U6 promoter sgRNA entry vector used for all NmeCas9 or Nme2Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Amrani et al. Genome Biology 2018
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Article
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Insert
    NmeCas9 and Nme2Cas9 sgRNA entry vector
    Expression
    Mammalian
    Mutation
    sgRNA architecture from Amrani et al. Genome Biol…
    Promoter
    U6
    Available Since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133794-plasmid-map-sequence-id-268076

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  11. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196084
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196084-plasmid-map-sequence-id-388993

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  14. DD-Cas9 with filler sequence and Cre-ERT2 (EDCICE)

    Plasmid
    #90086
    Purpose
    This plasmid contains destabilized Cas9 and has Cre-ERT2 after IRES sequence. This vector also contains filler sequence which required to be removed for cloning of desired sgRNA
    Depositor
    Raffaella Sordella
    Article
    Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10
    Insert
    Cas9
    Use
    CRISPR and Lentiviral
    Tags
    Destabilized Domain and Flag
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    90086-plasmid-map-sequence-id-182687

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  18. pPB-CAG-dCas9-VPR-wpA-pPuroTK

    Plasmid
    #214127
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Insert
    dCas9-VPR
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Available Since
    Feb. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    214127-plasmid-map-sequence-id-424240

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  20. pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196080
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Blasticidin S deaminase
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196080-plasmid-map-sequence-id-388988

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Showing: 2721 - 2740 of 14561 results