263,433 results

1. gRNA_AAVS1-T1

   Plasmid

   #41817

   Purpose

   Expresses a guide RNA (gRNA) to target human AAVS1 (T1 target sequence) for genome engineering

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Insert

   gRNA_AAVS1-T1
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. 3xFLAG-dCas9/pCMV-7.1

   Plasmid

   #47948

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al Biochem Biophys Res Commun. 2013 Sep 13;

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A (catalyticall…

   Promoter

   CMV

   Available Since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 3xFLAG-dCas9/pCMV-7.1

   Plasmid

   #47948

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al Biochem Biophys Res Commun. 2013 Sep 13;

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A (catalyticall…

   Promoter

   CMV

   Available Since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKrox24(2xD-E)DsRed

   Plasmid

   #200111

   Purpose

   Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   modified EGR1 promoter
   

   Expression

   Mammalian

   Mutation

   highly modified sequence

   Promoter

   2 copies of D-E element, no other promoter elemen…

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AAVS1 pegRNA2 with trimmed attP

   Plasmid

   #222347

   Purpose

   pegRNA 2 plasmid used for PASSIGE-mediated Bxb1 attP installation

   Depositor

   David Liu

   Article

   Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

   Insert

   AAVS1 attP-installation pegRNA2
   

   Use

   CRISPR

   Available Since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PCDH-WT-ALKBH5-3xFLAG

   Plasmid

   #203736

   Purpose

   Overexpression of ALKBH5-WT

   Depositor

   Jianjun Chen

   Article

   Shen et al Cell Stem Cell. 2020 Jul 2;27(1):64-80.e

   Insert

   ALKBH5 (ALKBH5 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   July 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-CFP

   Plasmid

   #13030

   Purpose

   Mammalian expression vector for cloning your gene fused to CFP

   Depositor

   Doug Golenbock

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Nov. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-CFP

   Plasmid

   #13030

   Purpose

   Mammalian expression vector for cloning your gene fused to CFP

   Depositor

   Doug Golenbock

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Nov. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. dCas9-dMSK1

   Plasmid

   #165602

   Purpose

   Expresses Sp dCas9 fused to truncated human MSK1 (42-802)

   Depositor

   Isaac Hilton

   Article

   Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1

   Insert

   S. Pyogenes dCas9 with c-terminal truncated human Mitogen- and stress-activated protein kinase-1 (42-802) (RPS6KA5 Human, S. Pyogenes, Synthetic)
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1-mhYFP

    Plasmid

    #186533

    Purpose

    Monomeric hyperfolder YFP fluorescent protein: cytosolic expression in mammalian cells

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    Monomeric hyperfolder YFP
    

    Expression

    Mammalian

    Mutation

    hfYFP-S147P/L195M/V206K

    Promoter

    CMV

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAM2991

    Plasmid

    #40248

    Depositor

    Susan Golden

    Article

    Ivleva et al EMBO J. 2005 Mar 23;24(6):1202-10. Epub

    Insert

    One Step Cloneing Vector for Overexpression
    

    Use

    Cyanobacteria cloning vector

    Promoter

    n/a

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAM2991

    Plasmid

    #40248

    Depositor

    Susan Golden

    Article

    Ivleva et al EMBO J. 2005 Mar 23;24(6):1202-10. Epub

    Insert

    One Step Cloneing Vector for Overexpression
    

    Use

    Cyanobacteria cloning vector

    Promoter

    n/a

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. plenti.CamKII.(synapto).iATPSnFR2.A95A.A119L.HaloTag

    Plasmid

    #209729

    Purpose

    Expresses presynaptic low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (synapto).iATPSnFR2.A95A.A119L.HaloTag
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CamKII

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. METTL3 shRNA 2 tet pLKO puro

    Plasmid

    #162984

    Purpose

    Tet-inducible shRNA targeting human METTL3 #2

    Depositor

    Mark Rubin

    Article

    Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

    Insert

    methyltransferase like 3 (METTL3 Human)
    

    Use

    Lentiviral

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMAL-XPG

    Plasmid

    #39215

    Depositor

    Aziz Sancar

    Article

    Matsunaga et al J Biol Chem. 1995 Sep 1;270(35):20862-9.

    Insert

    XPG (ERCC5 Human)
    

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Note that XPG starts at amino acid 5

    Promoter

    Ptac

    Available Since

    Aug. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMAL-XPG

    Plasmid

    #39215

    Depositor

    Aziz Sancar

    Article

    Matsunaga et al J Biol Chem. 1995 Sep 1;270(35):20862-9.

    Insert

    XPG (ERCC5 Human)
    

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Note that XPG starts at amino acid 5

    Promoter

    Ptac

    Available Since

    Aug. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFH2.9_MMLV_Gag-P2A-eGFP

    Plasmid

    #205524

    Purpose

    Express MMLV Gag-P2A-eGFP

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    Gag
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJMK001

    Plasmid

    #33780

    Depositor

    Adam Cohen

    Article

    Kralj et al Science. 2011 Jul 15;333(6040):345-8.

    Insert

    Proteorhodopsin Optical Proton Sensor
    

    Expression

    Bacterial

    Mutation

    D97N

    Promoter

    AraBAD

    Available Since

    Dec. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJMK001

    Plasmid

    #33780

    Depositor

    Adam Cohen

    Article

    Kralj et al Science. 2011 Jul 15;333(6040):345-8.

    Insert

    Proteorhodopsin Optical Proton Sensor
    

    Expression

    Bacterial

    Mutation

    D97N

    Promoter

    AraBAD

    Available Since

    Dec. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SMG6

    Plasmid

    #156020

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    SMG6 (SMG6 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge