-
Plasmid#160776PurposeSuppress CISD2DepositorInsertshCISD2_2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P CISD2_1
Plasmid#160775PurposeSuppress CISD2DepositorInsertshCISD2_1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
XE252 pCDNA3.1(zeo)-hDsh3-333-716
Plasmid#16757DepositorInsertFLAG-333-716-Dsh3 (DVL3 Human)
UseTagsFlagExpressionMammalianMutationaa 333-716PromoterAvailable sinceMarch 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
XE255 pCDNA3.1(zeo)-hDsh3-truncPDZ
Plasmid#16754DepositorInsertFLAG-hDsh3-trunc before PDZ (DVL3 Human)
UseTagsflagExpressionMammalianMutationtruncated before PDZPromoterAvailable sinceMarch 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-mNG-eDHFR(69K6)-Tiam1
Plasmid#172102PurposeMammalian expression of Tiam1(DH-PH) fused to mNeonGreen-eDHFR(69K6)DepositorInsertmNeonGreen-eDHFR(69K6)-Tiam1(DH-PH) (TIAM1 Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-JNKsub(TA)DD-YPet-NES
Plasmid#84634PurposeEncodes negative-control C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertJNKsub(TA)DD-YPet-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-GDNF
Plasmid#119897PurposeMouse GDNF In situ hybridization probeDepositorInsertglial cell line derived neurotrophic factor (Gdnf Mouse)
UseIn situ hybridization probeTagsExpressionMutationPromoterAvailable sinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
XE250 pCDNA3.1 + (zeo)- VSV-KLHL12-Q405X
Plasmid#16759DepositorInsertVSV-KLHL12-Q405X (KLHL12 Human)
UseTagsvsvExpressionMammalianMutationQ405STOPPromoterAvailable sinceMarch 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shEsco2
Plasmid#160957PurposeEsco2 shRNA in pMKO.1 retroviral vectorDepositorInsertEsco2 shRNA (Esco2 Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterU6Available sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_J
Plasmid#146709PurposeInsect Expression of DmNot1DepositorInsertDmNot1 (Not1 Fly)
UseTagsExpressionInsectMutationThree non silent mutations N605S, K759M, G1999S a…PromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_Ab-PiuA
Plasmid#128957Purposeexpresses Acinetobacter baumannii PiuA in E. coliDepositorInsertTonB-dependent siderophore receptor
UseTagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removedPromoterT7Available sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
3xFN-Tel-TAL/pMXs-puro
Plasmid#63590PurposeExpresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat. A retroviral expression vector with the puromycin resistance gene.DepositorInsert3xFVN-Tel-TAL
UseRetroviral and TALENTags3xFLAG-tag, V5-tag, and nuclear localization sign…ExpressionMammalianMutationPromoterLTRAvailable sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
S
Plasmid#167172PurposeExpresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant in Sf9 cellsDepositorInsertsATP1A1-S
ATP1B1
UseTagsExpressionBacterial and InsectMutationPromoterP10 and PHAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST_MAHS_RAMVA_81-236-FLAG (pBS0297)
Plasmid#185328PurposeFor the mammalian expression of the tardigrade protein MAHS_RAMVA_81-236-FLAG. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertMAHS_RAMVA_81-236-FLAG
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P CISD1_1
Plasmid#160773PurposeSuppress CISD1DepositorInsertshCISD1_1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL2 p15 4xSBR2 Mut 3' box
Plasmid#15708DepositorInsertp15 4xSBR2 (CDKN2B Human)
UseLuciferaseTagsExpressionMammalianMutationMutant 3' box. The 3' box contains the …PromoterAvailable sinceSept. 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shBub1
Plasmid#160948PurposeBub1 shRNA in pMKO.1 retroviral vectorDepositorInsertBub1 shRNA (Bub1 Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterU6Available sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
XE253 pCDNA3.1(zeo)-hDsh3-82-716
Plasmid#16756DepositorInsertFLAG-82-716-Dsh3 (DVL3 Human)
UseTagsflagExpressionMammalianMutationaa 82-716PromoterAvailable sinceMarch 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMV_SEPT9_i5-msfGFP
Plasmid#180327Purposemammalian expression of human SEPT9_i5 fused to monomeric superfolder GFPDepositorInsertSEPTIN9_v7 (SEPTIN9 Human)
UseTagsmsfGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shCdk1
Plasmid#160953PurposeCdk1 shRNA in pMKO.1 retroviral vectorDepositorInsertCdk1 shRNA (Cdk1 Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterU6Available sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-UBXD5-Strep-HA
Plasmid#113493PurposeExpression of human UBXD5 with C-terminal strep-HA tagDepositorInsertUBXD5 (UBXN11 Human)
UseTags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVL44 - pVND7>>
Plasmid#116010Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpVND7:GR-LhG4:tVND7:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseTagsHDEL and SP(ER)ExpressionPlantMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
DF-UAS-spa-GCaMP6f
Plasmid#67568PurposeZebrafish expression of a photoactivatable fluorescent calcium reporterDepositorInsertspa-GCaMP6f
UseZebrafish transgenesis (see :a spinal opsin contr…TagsHIS-tagExpressionMutationK65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…PromoterAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P MUTYH_2
Plasmid#160786PurposeSuppress MUTYHDepositorInsertshMUTYH_2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P MUTYH_1
Plasmid#160785PurposeSuppress MUTYHDepositorInsertshMUTYH_1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
px335-EF-Slc2a3-R
Plasmid#122305PurposeExpresses sgRNA targeting mouse Slc2a3 and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Slc2a3 (Slc2a3 Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCI-TPI_WT-xrRNA-4MS2-4H
Plasmid#108370PurposeExpresses wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag); 4MS2 tethering sites downstream of xrRNA and allows detection via northern blot (4H binding sites)DepositorInsertWild type TPI reporter with 4MS2 binding sites downstream of MVE xrRNA and 4H probe binding sites (TPI1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
XE254 pCDNA3.1(zeo)-hDsh3-497-716
Plasmid#16755DepositorInsertFLAG-497-716-Dsh3 (DVL3 Human)
UseTagsflagExpressionMammalianMutationaa 497-716PromoterAvailable sinceMarch 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJ4M_hnRNPA2_FL_P298L
Plasmid#139111PurposeExpress FL hnRNPA2 P298L with a C-terminal MBP tagDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsExpressionBacterialMutationP298LPromoterAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 PARL-FLAG-CT H335G
Plasmid#13749DepositorUseTagsFLAGExpressionMammalianMutationChanged catalytic His 335 to GlyPromoterAvailable sinceFeb. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEF-HA-TSAd-d239-74
Plasmid#84560Purposemammalian expression of TSAd-d239-74DepositorInsertTSAd (SH2D2A Human)
UseTagsHAExpressionMammalianMutationdeletion of aa 239-274PromoterEF1aAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
XE256 pCDNA3.1(zeo)-hDsh3-deltaCTER
Plasmid#16753DepositorInsertFLAG-hDsh3-deltaCTER (DVL3 Human)
UseTagsflagExpressionMammalianMutationdelta CTERPromoterAvailable sinceMarch 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pVL48 - pAt2g38380>>
Plasmid#116014Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpAT2G38380:GR-LhG4:tAt4g24550:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseTagsHDEL and SP(ER)ExpressionPlantMutationPromoterAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVL58 - pAPL>>
Plasmid#116015Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpAPL:GR-LhG4:tAPL:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseTagsHDEL and SP(ER)ExpressionPlantMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCI-TPI_WT-4MS2-xrRNA-4H
Plasmid#108371PurposeExpresses wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag); 4MS2 tethering sites upstream of xrRNA and allows detection via northern blot (4H binding sites)DepositorInsertWild type TPI reporter with 4MS2 binding sites upstream of MVE xrRNA and 4H probe binding sites (TPI1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVL41 - pSMXL5>>
Plasmid#116007Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpSMXL5:GR-LhG4:tSMXL5:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseTagsHDEL and SP(ER)ExpressionPlantMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC39A5
Plasmid#132057PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC39A5 (SLC39A5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVL45 - pPXY>>
Plasmid#116011Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpPXY:GR-LhG4:tPXY:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseTagsHDEL and SP(ER)ExpressionPlantMutationPromoterAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV10.F6.loxP
Plasmid#63201PurposepCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-PRAS40 F129A
Plasmid#86763PurposeN-terminal HA-tagged protein expression in mammalian cellsDepositorInserthuman PRAS40 (AKT1S1 Human)
UseTagsHAExpressionMammalianMutationF129APromoterCMVAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only