We narrowed to 15,991 results for: LEA

1. pACS57

   Plasmid

   #204582

   Purpose

   GST-lamin C (389-572)

   Depositor

   Tom Misteli

   Article

   Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

   Insert

   lamin C (389-572)
   

   Tags

   GST-lamin C (389-572)

   Expression

   Bacterial

   Available Since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. tagRFP-IMBB4-pUC

   Plasmid

   #170769

   Purpose

   test plasmid for 5RM assembly, encodes fluorescent red protein

   Depositor

   Ichiro Matsumura

   Article

   Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

   Insert

   tagRFP
   

   Promoter

   none

   Available Since

   Feb. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Venus-79aa-ActA-pEGFP-C1

   Plasmid

   #166763

   Purpose

   To target Venus to the mitochondrial outer membrane. ActA tail anchor inserts into the outer membrane and Venus faces the cytosol. Serves as a collisional control in FLIM-FRET experiments.

   Depositor

   David Andrews

   Insert

   ActA tail anchor (Listeria monocytogenes) (actA Synthetic, Listeria monocytogenes)
   

   Tags

   Venus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmCell-Free_KA1_SARS-COV-2_S-frag3

   Plasmid

   #169403

   Purpose

   Cell-free/mammalian expression vector for SARS-COV-2 virus N-term fragment of S protein after the furin cleavag with N-term eGFP

   Depositor

   Kirill Alexandrov

   Insert

   N-term frag. S protein after the furin cleavag (N SARS-Cov2)
   

   Use

   Cell-free expression vector

   Tags

   His and eGFP

   Promoter

   T7- CMV/CAG

   Available Since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmCellFree_KA1_SARS-COV-2_S-frag5

   Plasmid

   #169404

   Purpose

   Cell-free/mammalian expression vector for SARS-COV-2 virus C-terminal ectodomain of S protein from the furin cleavag with N-term eGFP

   Depositor

   Kirill Alexandrov

   Insert

   C-term frag. S protein after the furin cleavag (S SARS-Cov2)
   

   Use

   Cell-free expression vector

   Tags

   His and eGFP

   Promoter

   T7- CMV/CAG

   Available Since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBHRSF63

   Plasmid

   #118403

   Purpose

   for structure determination

   Depositor

   Hideo Iwai

   Article

   Iwai et al J Mol Biol. 2017 Dec 8;429(24):3942-3956

   Insert

   MvuTFIIB mini intein
   

   Expression

   Bacterial

   Available Since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBHDuet33

   Plasmid

   #118397

   Purpose

   analysis of cis-splicing

   Depositor

   Hideo Iwai

   Article

   Iwai et al J Mol Biol. 2017 Dec 8;429(24):3942-3956

   Insert

   MvuTFIIB intein
   

   Expression

   Bacterial

   Available Since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET15b-PvuRts1I

   Plasmid

   #86887

   Purpose

   express N-term His tagged PvuRts1I in ER2566 strain

   Depositor

   Matthias Bochtler

   Article

   Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

   Insert

   PvuRts1I (pvuRts1I )
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDL96

   Plasmid

   #43910

   Depositor

   Stephen Elledge

   Article

   Lee et al Mol Cell. 2008 Oct 10;32(1):70-80. doi:

   Insert

   DIF1 (DIF1 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   AAEAAA

   Available Since

   Aug. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

10. VTA

    Plasmid

    #27805

    Depositor

    Steven Vogel

    Article

    Koushik et al J Biomed Opt. 2008 May-Jun;13(3):031204.

    Insert

    Venus Traf Amber
    

    Expression

    Mammalian

    Available Since

    July 5, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pPS1523

    Plasmid

    #8919

    Depositor

    Pamela Silver

    Article

    Taura et al Proc Natl Acad Sci U S A 1998 Jun 23;95(

    Insert

    YRB2 (YRB2 Budding Yeast)
    

    Tags

    GFP

    Expression

    Yeast

    Available Since

    Aug. 19, 2005

    Availability

    Academic Institutions and Nonprofits only

12. pPS1356

    Plasmid

    #1379

    Depositor

    Pamela Silver

    Article

    Shen et al Genes Dev 1998 Mar 1;12(5):679-91.

    Insert

    NPL3 (NPL3 Budding Yeast)
    

    Tags

    Myc

    Expression

    Yeast

    Available Since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

13. pCas9-CR4

    Plasmid

    #62655

    Purpose

    Cas9 nuclease under control of pTet promoter with ssrA tag and constitutive tetR

    Depositor

    Kristala Prather

    Article

    Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

    Insert

    Cas9 nuclease
    

    Expression

    Bacterial

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-N1-Tspan4

    Plasmid

    #210323

    Purpose

    Expresses Tetraspanin-4 (TSPAN4) as migrasome marker

    Depositor

    Li Yu

    Article

    Ma et al Cell Res. 2015 Jan;25(1):24-38. doi: 10.

    Insert

    Tetraspanin 4 (Tspan4 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCWU6-xylR-E-luciferase

    Plasmid

    #190721

    Purpose

    fine tune expression of luciferase under the control of xylose- and theophylline- inducible system

    Depositor

    Chenggang Wu

    Article

    Bibek et al bioRxiv. 2023 Apr 24:2023.04.24.538132.

    Insert

    xylR-E-luciferase
    

    Use

    Luciferase

    Expression

    Bacterial

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAM1303

    Plasmid

    #40243

    Depositor

    Susan Golden

    Article

    Kulkarni et al Mol Microbiol. 1997 Jun;24(6):1131-42.

    Insert

    Sp/Sm Resistance, neutral site I integration platform for Synechococcus
    

    Use

    Cyanobacteria cloning vector

    Promoter

    n/a

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCS2+ EGFP-3NLS

    Plasmid

    #165400

    Purpose

    Expresses nuclear localized EGFP

    Depositor

    Philipp Niethammer

    Article

    Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

    Insert

    Nuclear Localization Signal
    

    Use

    Avian, xenopus, zebrafish

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    SP6

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag-RapGAP

    Plasmid

    #118324

    Purpose

    Expression of RapGAP

    Depositor

    Philip Stork

    Article

    Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

    Insert

    RapGAP
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. Cherry-LacRep

    Plasmid

    #18985

    Depositor

    Mirek Dundr

    Article

    Dundr et al J Cell Biol. 2007 Dec 17. 179(6):1095-10

    Insert

    LacI
    

    Tags

    AviTag, SV40 NLS, and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. HA-ISceID44A

    Plasmid

    #59424

    Purpose

    Expresses a catalytically inactive form of the endonuclease ISceI

    Depositor

    Tom Misteli

    Article

    Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

    Insert

    endonuclease ISceI mutant (I-SceI )
    

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    changed aspartate 44 to alanine (D44A)

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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