163,819 results

1. pEGFP VAMP7 (102-220)

   Plasmid

   #42314

   Depositor

   Thierry Galli

   Article

   Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

   Insert

   VAMP7 Coiled Coil domain (102-220) (VAMP7 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Δ1-102

   Promoter

   CMV

   Available since

   March 13, 2013

   Availability

   Academic Institutions and Nonprofits only

2. p2xFlag CMV2-YAP2-2nd WW mutant

   Plasmid

   #19047

   Depositor

   Marius Sudol

   Article

   Oka et al J Biol Chem. 2008 Jul 17. ():.

   Insert

   Yes-kinase associated protein (YAP1 Human)
   

   Use

   Tags

   2xFlag

   Expression

   Mammalian

   Mutation

   Tryptophan 258 changed to Alanine, and Proline 2…

   Promoter

   Available since

   Sept. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

3. P2A-mCherry-EhT backbone

   Plasmid

   #215549

   Purpose

   An empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of hygromycin-NLS-tdTomato

   Depositor

   Kai Wang

   Article

   Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PL452-hGH-polyA-PGK-neoR

   Plasmid

   #191681

   Purpose

   Donor plasmid backbone PGK-neoR

   Depositor

   Yuejun Chen

   Article

   Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. 5XUAS IVS CsChrimson tdtomato_tr

   Plasmid

   #111545

   Purpose

   Optogenetics in Drosophila: UAS-induced expression of Chrimson tdTomato

   Depositor

   Vivek Jayaraman

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   CsChrimson_tdtomato_trafficked
   

   Use

   Tags

   Expression

   Insect

   Mutation

   wt

   Promoter

   hsp70

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. 3CEG

   Plasmid

   #60107

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   BIRC6:SDC136-F10:C41228 (BIRC6 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   See comments

   Promoter

   T7

   Available since

   Oct. 22, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

7. TFORF1782

   Plasmid

   #143315

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZNF239 (ZNF239 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBEL1200

   Plasmid

   #138526

   Purpose

   For expresson of mlaFEDCB operon from E. coli. N-terminal his tag on MlaD, araBAD promoter.

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Ekiert et al Cell. 2017 Apr 6;169(2):273-285.e17. doi

   Insert

   mlaFEDCB (mlaF, mlaE, mlaD, mlaC, mlaB E. coli)
   

   Use

   Tags

   N-terminal His tag and TEV cleavage site on MlaD

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-Ef1a-fDIO-rc[Chronos-eGFP]-WPRE

   Plasmid

   #203847

   Purpose

   Flp-dependent expression of Chronos fused to EGFP

   Depositor

   Ivo Spiegel

   Article

   Cohen-Kashi Malina et al Neuron. 2021 Jul 7;109(13):2150-2164.e5.

   Insert

   Chronos
   

   Use

   AAV

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SCP1-pUC119

    Plasmid

    #21846

    Depositor

    James Kadonaga

    Article

    Juven-Gershon et al Nat Methods. 2006 Nov . 3(11):917-22.

    Insert

    SCP1 (super core promoter 1)-pUC119
    

    Use

    Puc derivative - a cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TFORF0318

    Plasmid

    #143392

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF117 (ZNF117 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. bdSUMO-pHD118-GFP-HiBit

    Plasmid

    #171015

    Purpose

    For bacterial expression of bdSUMO tagged muGFP with pHD118 peptide and NanoLuc complementation peptide variant #86

    Depositor

    Angus Johnston

    Article

    Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

    Insert

    14x-His-bdSUMO-pHD118-muGFP-HiBiT
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. bdSUMO-pHlip-GFP-HiBit

    Plasmid

    #171016

    Purpose

    For bacterial expression of bdSUMO tagged muGFP with pHlip peptide and NanoLuc complementation peptide variant #86

    Depositor

    Angus Johnston

    Article

    Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

    Insert

    14x-His-bdSUMO-pHlip-muGFP-HiBiT
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Multi-frame tag

    Plasmid

    #128610

    Purpose

    In the Multi-frame tag, HIV-1 frameshift sequence is upstream of FLAG epitopes in the 0 frame are separated from one another by SunTag epitopes in the -1 frame.

    Depositor

    Tim Stasevich

    Article

    Lyon et al Mol Cell. 2019 Jul 11;75(1):172-183.e9.

    Insert

    MF tag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pENTR-L1-PGK-PuroTK-L2

    Plasmid

    #141005

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    PuroTK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GFP(N-glyc)-Tac-TC

    Plasmid

    #162496

    Purpose

    Expresses partial length Tac (TMD and cytosolic tail) with GFP tag in the N-terminus in mammalian cells, and there is one N-glycosylation site in GFP tag.

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    partial length Tac (TMD and cytosolic tail) (IL2RA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Tac is in partial length with its TMD and cysotol…

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p3R112C/T180CBM3

    Plasmid

    #85090

    Purpose

    Expresses PCNA3 variant (R112C/T180C) fused to P450 BM3 variant (A74G/C773S/C810S) in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    The R112C/T180C variant of PCNA3 fused to P450 BM3
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C/T180C in PCNA3, A74G/C773S/C810S in P450BM3

    Promoter

    T7 promoter

    Available since

    Dec. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMaCTag-P21

    Plasmid

    #120032

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 24xSunTag

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    24xSunTag
    

    Use

    Pcr template

    Tags

    24xSunTag

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCoofy9

    Plasmid

    #122004

    Purpose

    E.coli vector for parallel SLIC cloning containing N-terminal StrepII with PreScission Cleavage site

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Use

    Tags

    StrepII

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ELAC1

    Plasmid

    #39097

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    ELAC1 (ELAC1 Human)
    

    Use

    Tags

    Tev His Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

21. pAG CMV Empty Puro

    Plasmid

    #237881

    Purpose

    Empty mammalian expression plasmid for cloning untagged proteins

    Depositor

    Addgene Research Program, Meghan Rego

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 12, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pcDNA3-Lyso-Digda

    Plasmid

    #236102

    Purpose

    Lysosome-targeted FRET-based biosensor for monitoring diacylglycerol (DAG) dynamics in living cells.

    Depositor

    Jin Zhang

    Article

    Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

    Insert

    Lyso-Digda (LAMP1 Synthetic, Human)
    

    Use

    Tags

    Full-length LAMP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCLJ-F2P-LoxP-PGK-NeoR-LoxP

    Plasmid

    #227503

    Purpose

    Inserts 500bp Prdm8 promoter in place of the Fgf5 promoter along with removable selection cassette

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    Prdm8 promoter
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV-BirA-HA-Tulp3

    Plasmid

    #236749

    Purpose

    Express BirA and HA tagged TULP3 transiently via transfection in mammalian cells.

    Depositor

    Zhao Zhang

    Article

    Jiang et al J Clin Invest. 2024 Aug 13;134(19):e1821

    Insert

    Tulp3 (Tulp3 Mouse)
    

    Use

    Tags

    BirA and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. EF1a-FRT-MCS-WPRE-SV40 - GCH15

    Plasmid

    #230822

    Purpose

    AAV cloning vector for Flpo-dependent expression under the EF1a promoter.

    Depositor

    Kimberly Ritola

    Type

    Empty backbone

    Use

    AAV; Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. EF1a-MCS-WPRE-SV40 - GCH13

    Plasmid

    #230820

    Purpose

    AAV cloning vector for expression under the EF1a promoter.

    Depositor

    Kimberly Ritola

    Type

    Empty backbone

    Use

    AAV; Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMK469 (mAID-dTAG BSD)

    Plasmid

    #214395

    Purpose

    mAID-dTAG for C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    mAID-dTAG-BSD
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA5-Flag/HA-TRBP-L326A/Y358A

    Plasmid

    #215892

    Purpose

    Expression of TRBP-L326A/Y358A

    Depositor

    Kumiko Ui-Tei

    Article

    Komori et al Biol Open. 2020 Feb 25;9(2):bio050435. d

    Insert

    TRBP-L326A/Y358A (TARBP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Lyn11-BirA*-HA

    Plasmid

    #214474

    Purpose

    Lentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with an N-terminal Lyn11 plasma membrane localization sequence and C-terminal HA tag.

    Depositor

    Natalie Ahn

    Article

    Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.

    Insert

    BirA(R118G)
    

    Use

    Lentiviral; Gateway

    Tags

    HA tag and Lyn11 (Plasma Membrane Localization Se…

    Expression

    Mammalian

    Mutation

    R118G

    Promoter

    EF-1a

    Available since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. TFORF0579

    Plasmid

    #143887

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF107 (ZNF107 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge