We narrowed to 76,436 results for: cha

1. BRCA1-(894-1057)

   Plasmid

   #35074

   Depositor

   Cheryl Arrowsmith

   Article

   Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

   Insert

   BRCA1 (BRCA1 Human)
   

   Use

   Tags

   His

   Expression

   Mutation

   Contains aa 894-1057

   Promoter

   T7

   Available since

   April 4, 2012

   Availability

   Academic Institutions and Nonprofits only

2. CS2 luc MutA

   Plasmid

   #13498

   Depositor

   Stephen Tapscott

   Article

   Rosenberg et al J Cell Biol. 2006 Oct 9. 175(1):77-85.

   Insert

   Fstl1 (Fstl1 Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   contains 3 tandem copies of the following sequenc…

   Promoter

   Available since

   Dec. 29, 2006

   Availability

   Academic Institutions and Nonprofits only

3. CS2 luc MutB

   Plasmid

   #13499

   Depositor

   Stephen Tapscott

   Article

   Rosenberg et al J Cell Biol. 2006 Oct 9. 175(1):77-85.

   Insert

   Fstl1 (Fstl1 Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   contains 3 tandem copies of the following sequenc…

   Promoter

   Available since

   Dec. 29, 2006

   Availability

   Academic Institutions and Nonprofits only

4. HM3C NaVSp1 pET24b

   Plasmid

   #128088

   Purpose

   Bacterial expression vector for NaVSp1 bacterial sodium channel. It expresses for HIStag-MBP-3C-channel protein

   Depositor

   Dan Minor

   Article

   Shaya et al Proc Natl Acad Sci U S A. 2011 Jul 26;10

   Insert

   NaVSp1p
   

   Use

   Tags

   3C Cleavage, His Tag, and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGST1-Osh4 (2-434)

   Plasmid

   #17632

   Depositor

   James Hurley

   Article

   Im et al Nature. 2005 Sep 1. 437(7055):154-8.

   Insert

   Osh4 (KES1 Budding Yeast)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 28, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pR5-28

   Plasmid

   #22289

   Depositor

   Louise Prakash

   Article

   Pages et al Genetics. 2008 Sep . 180(1):73-82.

   Insert

   RAD5 (RAD5 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 8, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pGFP-ATG13(315)

   Plasmid

   #122072

   Purpose

   Expressing GFP-Atg13

   Depositor

   Daniel Klionsky

   Article

   Chew et al Autophagy. 2015;11(6):891-905. doi: 10.1

   Insert

   ATG13
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAW12

   Plasmid

   #110224

   Purpose

   integration of ura4+-loxM3 cassette

   Depositor

   Tony Carr

   Article

   Watson et al Gene. 2008 Jan 15;407(1-2):63-74

   Insert

   ura4+ (ura4 Fission Yeast)
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   See depositor comments below.

   Promoter

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pR5-30

   Plasmid

   #22290

   Depositor

   Louise Prakash

   Article

   Pages et al Genetics. 2008 Sep . 180(1):73-82.

   Insert

   RAD5 (RAD5 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   D681, E682->AA

   Promoter

   Available since

   Nov. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

10. piHGpd/P82

    Plasmid

    #1325

    Depositor

    Susan Lindquist

    Article

    Nathan et al Proc Natl Acad Sci U S A 1997 Nov 25;94(

    Insert

    GPD promoter-HSP82 (HSP82 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Wild type gene

    Promoter

    Available since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

11. pDONR221-Sgd1

    Plasmid

    #100000

    Purpose

    Shuttle Sgd1 into Gateway destination vector

    Depositor

    Susan Baserga

    Article

    Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

    Insert

    Sgd1 (SGD1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDONR221-Bms1

    Plasmid

    #100001

    Purpose

    Shuttle Bms1 into Gateway destination vector

    Depositor

    Susan Baserga

    Article

    Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

    Insert

    Bms1 (BMS1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDONR221-Ckb1

    Plasmid

    #99981

    Purpose

    Shuttle yeast Ckb1 into Gateway destination vector

    Depositor

    Susan Baserga

    Article

    Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

    Insert

    Ckb1 (CKB1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDONR221-Ckb2

    Plasmid

    #99982

    Purpose

    Shuttle yeast Ckb2 into Gateway destination vector

    Depositor

    Susan Baserga

    Article

    Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

    Insert

    Ckb2 (CKB2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR221-Utp11

    Plasmid

    #99984

    Purpose

    Shuttle yeast Utp11 into Gateway destination vector

    Depositor

    Susan Baserga

    Article

    Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

    Insert

    Utp11 (UTP11 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDONR221-Pfa1

    Plasmid

    #99999

    Purpose

    Shuttle yeast Pfa1 into Gateway destination vector

    Depositor

    Susan Baserga

    Article

    Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

    Insert

    Pfa1 (SQS1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDONR221-Cka1

    Plasmid

    #99979

    Purpose

    Shuttle yeast Cka1 into Gateway destination vector

    Depositor

    Susan Baserga

    Article

    Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

    Insert

    Cka1 (CKA1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti-U6-tdTomato-P2A-BlasR (LRT2B)

    Plasmid

    #110854

    Purpose

    Lentiviral vector for constitutive expression of sgRNAs; includes tdTomato and BlasticidinS resistance markers

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    sgRNA scaffold with spacer
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    U6

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. DLX2-hygro

    Plasmid

    #97330

    Purpose

    Generating lentiviruses to express Dlx2 and hygromycin resistance

    Depositor

    Marius Wernig

    Article

    Yang et al Nat Methods. 2017 May 15. doi: 10.1038/n

    Insert

    DLX2 (Dlx2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TetO

    Available since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TetO-Ascl1-puro

    Plasmid

    #97329

    Purpose

    Generating lentiviruses to express Ascl1 and puromycin resistance

    Depositor

    Marius Wernig

    Article

    Yang et al Nat Methods. 2017 May 15. doi: 10.1038/n

    Insert

    Ascl1 (Ascl1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TET-ON

    Available since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. EGFP-COV E

    Plasmid

    #165123

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    COV E (E codon optimized SARS-COV-2)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTRE3G-Bi-gePSI-pCamk2a-AcGFP

    Plasmid

    #133732

    Purpose

    Expresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a Camk2a promoter.

    Depositor

    Erin Schuman

    Article

    Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

    Inserts

    gePSI-β-chain
    gePSI-α-chain
    AcGFP1
    

    Use

    AAV

    Tags

    murine ornithine decarboxylase - degron (ODC36)

    Expression

    Mammalian

    Mutation

    Promoter

    pCamk2a and pTRE3G-Bi

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. FAT FAK biosensor

    Plasmid

    #78303

    Purpose

    FRET biosensor. Visualization of FAK activity at focal adhesions in live cells

    Depositor

    Yingxiao Wang

    Article

    Seong et al Proc Natl Acad Sci U S A. 2013 Nov 26;11

    Insert

    FAT FAK biosensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

24. pMXs-Fos

    Plasmid

    #131595

    Purpose

    expression of mouse Fos in mammalian cells

    Depositor

    Kateri Moore, Filipe Pereira

    Article

    Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

    Insert

    Fos (Fos Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pML104

    Plasmid

    #67638

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation

    Depositor

    John Wyrick

    Article

    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

    Inserts

    Cas9
    single guide RNA expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pSNR52 and pTDH3

    Available since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCW57.1 tTA UQCRC2

    Plasmid

    #177978

    Purpose

    All-in-one dox-repressible expression of cDNA

    Depositor

    David Sabatini, Jessica Spinelli

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    UQCRC2 (UQCRC2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Silent point mutations to UQCRC2 sgRNA target

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCW57.1 tTA SDHB

    Plasmid

    #177977

    Purpose

    All-in-one dox-repressible expression of cDNA

    Depositor

    David Sabatini, Jessica Spinelli

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    SDHB (SDHB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Silent point mutations to SDHB sgRNA target

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pML107

    Plasmid

    #67639

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation

    Depositor

    John Wyrick

    Article

    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

    Inserts

    Cas9
    single guide RNA expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pSNR52 and pTDH3 (aka GAP promoter)

    Available since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. a1Ha-pcDNA3

    Plasmid

    #45809

    Depositor

    Edward Perez-Reyes

    Article

    Cribbs et al Circ Res. 1998 Jul 13;83(1):103-9.

    Insert

    a1Ha (CACNA1H Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJBEI-6409

    Plasmid

    #47048

    Purpose

    BglBrick plasmid (=pBbA5c-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli

    Depositor

    Taek Soon Lee

    Article

    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

    Insert

    Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene
    

    Use

    Synthetic Biology; Bglbrick

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacUV5

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pEVOL-ABK

    Plasmid

    #126035

    Purpose

    Efficient expression of tRNA synthetase/tRNA for the incorporation of a photo-cross-linker, 3’-azibutyl-N-carbamoyl-lysine (AbK) into recombinant protein by amber codon (TAG) suppression in E. coli.

    Depositor

    Andrea Musacchio

    Article

    Pan et al Nat Commun. 2019 Sep 6;10(1):4046. doi:

    Inserts

    tRNA synthetase 1
    tRNA synthetase 2
    tRNA for TAG codon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    L274M, C313A, Y349F

    Promoter

    araBAD promoter, glnS promoter, and proK promoter

    Available since

    Sept. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. EGFP-COV M

    Plasmid

    #165124

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    COV M (M codon optimized SARS-COV-2)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. LBR pEGFP-N2 (646)

    Plasmid

    #61996

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    LBR (LBR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pTRE3G-Bi-gePSI-pGFAP-AcGFP

    Plasmid

    #133733

    Purpose

    Expresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a GFAP promoter.

    Depositor

    Erin Schuman

    Article

    Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

    Inserts

    gePSI-β-chain
    gePSI-α-chain
    AcGFP1
    

    Use

    AAV

    Tags

    murine ornithine decarboxylase - degron (ODC36)

    Expression

    Mammalian

    Mutation

    Promoter

    pGFAP and pTRE3G-Bi

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTRE3G-Bi-gePSI-pCMV-AcGFP

    Plasmid

    #131008

    Purpose

    Expresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a CMV promoter.

    Depositor

    Erin Schuman

    Article

    Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

    Inserts

    gePSI-β-chain
    gePSI-α-chain
    AcGFP1
    

    Use

    AAV

    Tags

    murine ornithine decarboxylase - degron (ODC36)

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV and pTRE3G-Bi

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLenti-ABERA-P2A-Puro

    Plasmid

    #112675

    Purpose

    Lentivirus for constitutive expression of ABE7.10 RA (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    ABERA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    D10A

    Promoter

    EF1s

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. PiggyBac-TetO-Ascl1-neomycin

    Plasmid

    #176482

    Purpose

    TetO-Ascl1 in a PiggyBac vector with neomycin resistance

    Depositor

    Marius Wernig

    Article

    Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

    Insert

    Ascl1 (Ascl1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEGFP VARP

    Plasmid

    #42312

    Depositor

    Thierry Galli

    Article

    Burgo et al EMBO Rep. 2009 Oct;10(10):1117-24. doi:

    Insert

    VARP (ANKRD27 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

39. pOPINB-hRNF216

    Plasmid

    #167354

    Purpose

    Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells

    Depositor

    Bernhard Lechtenberg

    Article

    Cotton et al Mol Cell. 2022 Feb 3;82(3):598-615.e8. d

    Insert

    E3 ubiquitin-protein ligase RNF216 (RNF216 Human)
    

    Use

    Tags

    3C protease cleavage site and 6x-His tag

    Expression

    Bacterial

    Mutation

    codon optimised for expression in E. coli

    Promoter

    T7

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pIRES2Cx32WTEGFP

    Plasmid

    #78150

    Purpose

    Mammalian Expression of Connexin 32 and EGFP from a bicistronic mRNA

    Depositor

    Mario Bortolozzi

    Article

    Carrer et al Hum Mol Genet. 2017 Oct 25. pii: 4565496

    Insert

    Connexin 32 (GJB1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge