We narrowed to 24,878 results for: Spr

1. pU6-gRNA

   Plasmid

   #53062

   Purpose

   Transcription of guide RNA (gRNA) in wheat cells

   Depositor

   Caixia Gao

   Article

   Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

   Insert

   wheat U6 promoter - gRNA
   

   Use

   CRISPR; Plant expression

   Promoter

   wheat U6

   Available Since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBAD_TypeIC_Cascade_cas3_invivo

   Plasmid

   #196400

   Purpose

   expresses CRISPR Cascade-Cas3 Type IC proteins for in vivo assay

   Depositor

   David Taylor

   Article

   O'Brien et al Mol Cell. 2023 Mar 2;83(5):746-758.e5. d

   Inserts

   Cas5c
   Cas7c
   Cas8c
   Cas3c
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC18-mini-Tn7T-Plac-dCas9 (Plac)

   Plasmid

   #105235

   Purpose

   Tn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Plac promoter for expression in P. aeruginosa

   Depositor

   Kristala Prather

   Article

   Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

   Insert

   S. pasteurianus dCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   HA

   Expression

   Bacterial

   Mutation

   D10A, H599A

   Available Since

   Aug. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYC1640

   Plasmid

   #158719

   Purpose

   CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using zeocin as a selection marker.

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   Sth1 sgRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpCas9-mSA*

   Plasmid

   #182952

   Purpose

   Express SpCas9-mSA*

   Depositor

   Wen Xue

   Article

   Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

   Insert

   SpCas9-mSA*
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCpf1b

   Plasmid

   #122187

   Purpose

   Bacterial genome editing plasmid with kanamycin resistance marker and using sacB as a counter-selection marker

   Depositor

   Cheng-Cai Zhang

   Article

   Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV hU6-gRNA(anti-sense) hUbC-VP64-dCas9-VP64-T2A-GFP

   Plasmid

   #66707

   Purpose

   expresses a single gRNA and VP64-dCas9-VP64

   Depositor

   Charles Gersbach

   Inserts

   hU6-gRNA expression cassette
   S.Pyogenes VP64-dCas9-VP64
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   VP64

   Mutation

   D10A and H840A

   Promoter

   hU6 and hUbC

   Available Since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQAT60

   Plasmid

   #223432

   Purpose

   T-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by 2x35s and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   2x35s-dSpCas9-Act3.0-OsU3-gRNA scaffold 2.0
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   June 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQAT58

   Plasmid

   #223430

   Purpose

   T-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-dSpCas9-Act3.0-OsU3-gRNA scaffold 2.0
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQAT61

    Plasmid

    #223433

    Purpose

    T-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1 promoter; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-dSpCas9-Act3.0-ZmUbi-gRNA scaffold 2.0-tRNA
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CASI-Cas9C-P2A-turboGFP

    Plasmid

    #80942

    Purpose

    Expresses Cas9C in mammalian cells; co-expresses turboGFP.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9C
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. U6-spTRE3G-CMV-mTagBFP2

    Plasmid

    #102854

    Purpose

    A plasmid encoding TRE3G sgRNA (for SpCas9) and mTagBFP

    Depositor

    Michael Lin

    Article

    Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

    Insert

    sp-TRE3G-sgRNA; mTagBFP2
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPBO.ACT001

    Plasmid

    #182711

    Purpose

    aTc inducible dCasRx

    Depositor

    Joseph Schoeniger

    Article

    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

    Insert

    dCasRx
    

    Use

    CRISPR

    Tags

    2x-FLAG

    Expression

    Bacterial

    Mutation

    Catalytically deactivated R295A, H300A, R849A, H8…

    Promoter

    pTet

    Available Since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJL33

    Plasmid

    #227430

    Purpose

    Kanamycin resistant L5-integrative vector for CRISPR interference in M.tuberculosis with the constitutive expression of mWasabi under the control of the psmyc promotor (psmyc::mWasabi)

    Depositor

    Pierre Santucci

    Article

    Laudouze et al FEBS Lett. 2025 Feb;599(4):488-501. doi:

    Insert

    mWasabi
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJL34

    Plasmid

    #227431

    Purpose

    Kanamycin resistant L5-integrative vector for CRISPR interference in M.tuberculosis with the constitutive expression of tdTomato under the control of the psmyc promotor (psmyc::tdTomato)

    Depositor

    Pierre Santucci

    Article

    Laudouze et al FEBS Lett. 2025 Feb;599(4):488-501. doi:

    Insert

    tdTomato
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPIPL12-FnCas12a

    Plasmid

    #199563

    Purpose

    Tetracycline-Inducible Fncas12a gene expression in E. coli and clostridia

    Depositor

    Philippe Lambin

    Article

    Zhang et al Microbiol Spectr. 2023 Nov 10:e0245923.

    Insert

    Fncas12a
    

    Use

    CRISPR and Synthetic Biology; Tetracycline induci…

    Expression

    Bacterial

    Promoter

    COtetR-PIPL12

    Available Since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKLV2-U6gRNASAM(gTetO)-TREGFP-PGKpuroBFP-W

    Plasmid

    #112927

    Purpose

    CRISPR-a reporter assay lentiviral vector (gTetO)

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    hU6-gRNASAM(gTetO), TREGFP, PGKpuroGFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-itis pSel

    Plasmid

    #195344

    Purpose

    Selection plasmid expressing non-fluorescent EGFP A111V

    Depositor

    Alexis Komor

    Article

    Vasquez et al CRISPR J. 2023 Apr 20. doi: 10.1089/cris

    Insert

    EGFP
    

    Use

    CRISPR

    Mutation

    A111V

    Promoter

    J23119

    Available Since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CASI-Cas9C

    Plasmid

    #80932

    Purpose

    Expresses Cas9C in mammalian cells; derived from pZac2.1 with CASI promoter.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9C
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYL-kasOp-FnCas12a2

    Plasmid

    #177874

    Purpose

    Expresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.

    Depositor

    Yunzi Luo

    Article

    Zhang et al Front Bioeng Biotechnol. 2020 Jun 30;8:7

    Insert

    Streptomyces codon optimized FnCas12a
    

    Use

    CRISPR

    Promoter

    kasOp*

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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