We narrowed to 8,402 results for: PROC

1. pRK-ATF4 111-351

   Plasmid

   #26119

   Depositor

   Yihong Ye

   Article

   Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

   Insert

   ATF4 (ATF4 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   aa's 1-110 have been deleted

   Promoter

   Available since

   Dec. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

2. MigR1-AEtr

   Plasmid

   #12432

   Depositor

   Dong-Er Zhang

   Article

   Yan et al Proc Natl Acad Sci U S A. 2004 Dec 7. 10

   Insert

   C-term truncated AML1-ETO (RUNX1, RUNX1T1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

3. mApple-proα2(I)

   Plasmid

   #119840

   Purpose

   Expresses mouse Type I procollagen α2 chain (Col1a2) with mApple between signal sequence and exon 6

   Depositor

   Sergey Leikin

   Article

   Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

   Insert

   Type I procollagen α2 chain (Col1a2 Mouse)
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   Col1a2 exons 2-5 replaced by fluorescent tag

   Promoter

   CMV

   Available since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCDNA3/FKBP12-pBirA(284-C)-HA

   Plasmid

   #154452

   Purpose

   expresses FKBP12-pBirA(284-C)-HA/biochemical assay

   Depositor

   Hyun-Woo Rhee

   Article

   Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

   Insert

   FKBP12-pBirA(284-C)-HA (FKBP12 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRK-ATF4 248-351

   Plasmid

   #26120

   Depositor

   Yihong Ye

   Article

   Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

   Insert

   ATF4 (ATF4 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   ATF4 aa's 1-247 have been deleted

   Promoter

   Available since

   Jan. 31, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pAAV-CAG-LMO3 (sbGLuc-VChR1-EYFP)

   Plasmid

   #114104

   Purpose

   fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

   Insert

   sbGLuc-VChR1-EYFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAD54_CAD29_H104A_pvp008

   Plasmid

   #124876

   Purpose

   Expresses hCAD His103Ala mutant in E.coli

   Depositor

   Konrad Buessow

   Article

   Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

   Insert

   cis-aconitate decarboxylase (ACOD1 Human)
   

   Use

   Tags

   StrepTag and TEV site

   Expression

   Bacterial

   Mutation

   mutated His103 to Ala, deleted amino acids 1-3 an…

   Promoter

   T7

   Available since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pZE21/UBP1/ClpS

   Plasmid

   #98566

   Purpose

   Expresses truncated codon-opt S. cerevisiae UBP1 and E. coli ClpS in an artificial operon

   Depositor

   George Church

   Article

   Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11

   Inserts

   Truncated ubiquitin cleavase, codon optimized
   ATP-dependent Clp protease adapter protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   In a synthetic operon downstream of UBP1 and Remo…

   Promoter

   Same as UBP1 (operon) and Tet promoter (aTc induc…

   Available since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMXs-ms-c-Myc-L420P

   Plasmid

   #50776

   Purpose

   retroviral expression of mouse c-Myc L420P mutant

   Depositor

   Shinya Yamanaka

   Article

   Nakagawa et al Proc Natl Acad Sci U S A. 2010 Jul 26. (

   Insert

   c-Myc L420P (MYC, Myc Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   L420P (does not bind to Max)

   Promoter

   Available since

   Feb. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mature ISG15 C-term. domain (79-157)

    Plasmid

    #110764

    Purpose

    Bacterial expression of proISG15 C-term. domain

    Depositor

    David Komander

    Article

    Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

    Insert

    ISG15 (ISG15 Human)
    

    Use

    Tags

    N-His6-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCEP4-myc-HLA-A2-VN

    Plasmid

    #135485

    Purpose

    Mammalian expression of VN-fused and myc-tagged HLA-A*02:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*02:01 (HLA-A Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag, Signal peptide f…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. proISG15 C-term. domain (79-165)

    Plasmid

    #110763

    Purpose

    Bacterial expression of proISG15 C-term. domain

    Depositor

    David Komander

    Article

    Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

    Insert

    ISG15 (ISG15 Human)
    

    Use

    Tags

    N-His6-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CFP-GIV-CT FA

    Plasmid

    #69533

    Purpose

    Mammalian expression of The CFP-tagged GIV-CT-FA biosensors

    Depositor

    Pradipta Ghosh

    Article

    Midde et al Proc Natl Acad Sci U S A. 2015 Mar 3;112

    Insert

    GIV (CCDC88A Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    C terminal amino acids S1660-S1870 of human GIV w…

    Promoter

    CMV

    Available since

    Jan. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p029 End-1::EnR

    Plasmid

    #11029

    Depositor

    Ramesh Shivdasani

    Article

    Shoichet et al Proc Natl Acad Sci U S A. 2000 Apr 11. 9

    Insert

    End1::EnR (end-1, en END-1 is from C. Elegans, EnR is from Drophila)
    

    Use

    Xenopus, mammalian, avian, and zebrafish

    Tags

    Expression

    Mutation

    Dominant inhibitory GATA-binding protein End1::En…

    Promoter

    Available since

    Dec. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mOrange-P2A-hKvb1

    Plasmid

    #154084

    Purpose

    Independent expression of mOrange and human Kvbeta 1 under human synapsin 1 promoter

    Depositor

    Michael Hoppa

    Article

    Cho et al Proc Natl Acad Sci U S A. 2020 Nov 24;11

    Insert

    mOrange-P2A-human Kvb1 (KCNAB1 Synthetic, Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin 1

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSUMO His6 SUMO SnRK2.2 (20-362)

    Plasmid

    #177845

    Purpose

    Bacterial Expression of SnRK2.2

    Depositor

    Karsten Melcher

    Article

    Ng et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

    Insert

    SNF1-related protein kinase 2 (SNRK2.2 Mustard Weed)
    

    Use

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRetroX GFP Mps1 DK

    Plasmid

    #63703

    Purpose

    Inducible expression of GFP-Mps1 DK fusion in mammalian cells

    Depositor

    Floris Foijer

    Article

    Foijer et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

    Insert

    Mps1 (TTK Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deletion of aa 47-156, kinetochore binding domain

    Promoter

    tight TRE promoter

    Available since

    April 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Flag_MmPERK_S503_N1114_pBabePuro

    Plasmid

    #58423

    Purpose

    retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant mouse PERK deleted from amino acids M1 to Y502. The remaining amino acids are S503 to N1114.

    Depositor

    David Ron

    Article

    Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11

    Insert

    PERK (Eif2ak3 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG and preprotrypsin signal sequence

    Expression

    Mammalian

    Mutation

    Deleted amino acids M1 to Y502

    Promoter

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HOXB6 (human) FLAG pMSCV

    Plasmid

    #8534

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB6 (HOXB6 Human)
    

    Use

    Retroviral

    Tags

    FLAG and IRES-GFP

    Expression

    Mammalian

    Mutation

    contains improved ribosomal binding site and FLAG…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pCR-Myc-IKKalpha

    Plasmid

    #19739

    Depositor

    Hiroyasu Nakano

    Article

    Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

    Insert

    IkB kinase alpha (Chuk Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

21. pGL2-hOct4 DE-SV40-Luc

    Plasmid

    #26279

    Purpose

    Addgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.

    Depositor

    Rudolf Jaenisch

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

    Insert

    Oct4 Distal Enhancer Element (DE) (POU5F1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments below

    Promoter

    Available since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. mCerulean-proα2(I)-G610C

    Plasmid

    #119837

    Purpose

    Expresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mCerulean between signal sequence and exon 6

    Depositor

    Sergey Leikin

    Article

    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

    Insert

    Type I procollagen α2 chain (Col1a2 Mouse)
    

    Use

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    Col1a2 exons 2-5 replaced by fluorescent tag, Gly…

    Promoter

    CMV

    Available since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. mVenus-proα2(I)

    Plasmid

    #119838

    Purpose

    Expresses mouse Type I procollagen α2 chain (Col1a2) with mVenus between signal sequence and exon 6

    Depositor

    Sergey Leikin

    Article

    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

    Insert

    Type I procollagen α2 chain (Col1a2 Mouse)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Col1a2 exons 2-5 replaced by fluorescent tag

    Promoter

    CMV

    Available since

    Jan. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. HOXB5 (human) FLAG-MSCV

    Plasmid

    #8532

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB5 (HOXB5 Human)
    

    Use

    Retroviral

    Tags

    FLAG and IRES-GFP

    Expression

    Mammalian

    Mutation

    contains improved ribosomal binding site and FLAG…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

25. EFNB2-tdTomato

    Plasmid

    #139967

    Purpose

    Donor vector to knockin tdTomado into EFNB2 allele

    Depositor

    James Thomson

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jul 25;11

    Insert

    Human EFNB2 knockin homology arms (EFNB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1_SNORD27h_2ntmut

    Plasmid

    #73067

    Purpose

    expression clone for human mutated SNORD27 (C/D box snoRNA U27)

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    SNORD27 (E2F7 Human)
    

    Use

    Tags

    no tag

    Expression

    Mammalian

    Mutation

    2 nt mutation in AS box that binds E2F7 pre-mRNA

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

27. tdEos-Actin-C-18

    Plasmid

    #57571

    Purpose

    Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

    Insert

    Actin (ACTB Human)
    

    Use

    Tags

    tdEos

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

28. WT MetNIQ in pBAD

    Plasmid

    #118253

    Purpose

    Wild Type Methionine Binding Protein and Methionine ABC Transporter in pBAD Vector for Transport Activity Assay

    Depositor

    Douglas Rees

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

    Inserts

    MetN (metN E. coli)
    MetI (metI E. coli)
    MetQ (metQ E. coli)
    

    Use

    Tags

    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

    Expression

    Bacterial

    Mutation

    Changed Glutamic Acid (Glu) 295 to Alanine (Ala)

    Promoter

    None and araBAD Promoter

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1_hE2F7mg_PP7-tag

    Plasmid

    #73078

    Purpose

    human E2F7 minigene (exons 11,12,13/introns cassette) with PP7-tag on 5'-end

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    E2F7 (E2F7 Human)
    

    Use

    Tags

    5'-PP7-tag RNA

    Expression

    Mammalian

    Mutation

    partial gene, exons 11,12,13 spaced by introns

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. mApple-proα2(I)-G610C

    Plasmid

    #119841

    Purpose

    Expresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mApple between signal sequence and exon 6

    Depositor

    Sergey Leikin

    Article

    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

    Insert

    Type I procollagen α2 chain (Col1a2 Mouse)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Col1a2 exons 2-5 replaced by fluorescent tag, Gly…

    Promoter

    CMV

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge