We narrowed to 11,222 results for: NSI
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Plasmid#195897PurposeLevel 0 tUBQ1 terminator with GG4 GG6 golden gate linkersDepositorInserttUBQ1 (UBQ1 Mustard Weed)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
Linked Free NES (M)
Plasmid#182436PurposeYeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertFPPS(M)-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed Free NES (M)
Plasmid#182437PurposeYeast integrative plasmid for expressing ERG20(F96W-N127W) (GAL10 promoter) and AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsFPPS(M)
NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10 and GAL7Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRD415
Plasmid#178197PurposeRok _Linker overexpressionDepositorInsertrok _linker
UseTagsExpressionBacterialMutationdeletion of residues 75-96PromoterT7Available sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRD461
Plasmid#178198PurposeRok with a C-terminal His-tag overexpressionDepositorInsertrok 6xHis
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-mCherry-HRasCT
Plasmid#186704PurposeEncoding membrane-targeted mCherryDepositorInsertmCherry-HRasCT
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0544 CMV-TO-mCherry-SG(s70587-dblstop)SG-EGFP (FLP-IN)
Plasmid#191176PurposeMammalian expression of EK0209 with a second stop codon.DepositorInsertCMV-TO:mCherry:s70587:EGFP (double-stop)
UseTagsExpressionMammalianMutationadditional stop codonPromoterCMV-TOAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0546 SFFV-mCherry-SGc(GAPDH.s1-dblstop)cSG-EGFP-(3xMS2) (FLP-IN)
Plasmid#191178PurposeMammalian expression of EK0493 with second stop codon.DepositorInsertmCherry:GAPDH.s1:EGFP (double-stop)
UseTagsExpressionMammalianMutationadditional stop codonPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0344 SFFV-mCherry-SG(Bdnf.s1)SG-Cre (FLP-IN)
Plasmid#191158PurposeExpression of a sensor for murine Bdnf 3' UTR in mammalian cells; mCherry marker, Cre output; 90 bp longDepositorInsertmCherry:Bdnf.s1:Cre
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
NSK025 CMV-TO-EGFP:c.637A>G(N213D; CCA>CCG)-stop-t70587 (PB)
Plasmid#191166PurposeMammalian expression of a EGFP mutant N213D derived from EK0379.DepositorInsertEGFP(N213D)
UseTagsExpressionMammalianMutationc.637A>G(N213D; CCA>CCG)PromoterCMV-TOAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0362 SFFV-mCherry-SG(Bdnf.s1L36+54@202)SG-EGFP (FLP-IN)
Plasmid#191153PurposeMammalian expression of a 90 bp (36+54) long sensor for murine Bdnf 3' UTR where the trigger sequence is splitDepositorInsertmCherry:Bdnf.s1L36+54:EGFP
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPbaCas13b-EGFP-NLS
Plasmid#191365PurposeOverexpression of NLS-dPbaCas13b-EGFP-NLS in human cellsDepositorInsertNLS-dPbaCas13b-EGFP-NLS
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPba5Cas13b-EGFP-NLS
Plasmid#191364PurposeOverexpression of NLS-dPba5Cas13b-EGFP-NLS in human cellsDepositorInsertNLS-dPba5Cas13b-EGFP-NLS
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPba4Cas13b-EGFP-NLS
Plasmid#191363PurposeOverexpression of NLS-dPba4Cas13b-EGFP-NLS in human cellsDepositorInsertNLS-dPba4Cas13b-EGFP-NLS
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dHgm6Cas13b-EGFP-NLS
Plasmid#191366PurposeOverexpression of NLS-dHgm6Cas13b-EGFP-NLS in human cellsDepositorInsertNLS-dHgm6Cas13b-EGFP-NLS
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dBba2Cas13b-EGFP-NLS
Plasmid#191362PurposeOverexpression of NLS-dBba2Cas13b-EGFP-NLS in human cellsDepositorInsertNLS-dBba2Cas13b-EGFP-NLS
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPba3Cas13b-EGFP-NLS
Plasmid#191361PurposeOverexpression of NLS-dPba3Cas13b-EGFP-NLS in human cellsDepositorInsertNLS-dPba3Cas13b-EGFP-NLS
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMM4_14
Plasmid#183062PurposePlasmid expressing a biosensor for acetic acid: BM3R1-Haa1-mTurquoise2 under RET2p and mCherry under HREsYGP1p with flanks for genome integration into HO locusDepositorInsertsmCherry
BM3R1-HAA1-mTurquoise2
UseSynthetic BiologyTagsmCherry and mTurquoise2ExpressionYeastMutationPromoterHREsYGP1p and RET2Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB9339 (pgRNA_VII-1_NatMX)
Plasmid#161591PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site VII-1DepositorInsertguiding RNA
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB9340 (pgRNA_VIII-1_NatMX)
Plasmid#161592PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site VIII-1DepositorInsertguiding RNA
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB9342 (pgRNA_XIII-1_NatMX)
Plasmid#161594PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XIII-1DepositorInsertguiding RNA
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB9344 (pgRNA_XVI-1_NatMX)
Plasmid#161596PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XVI-1DepositorInsertguiding RNA
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNCS-Clover4-LUMABS-HIV
Plasmid#183485PurposeTo express the Clover4-LUMABS-HIV sensor for Acquired Immunodeficiency Virus antibody dection.DepositorInsertClover4-LUMABS-HIV
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-Clover4-LUMABS-S1 RBD
Plasmid#183491PurposeTo express the Clover4-LUMABS-S1 RBD sensor for SARS-CoV-2 antibody dection.DepositorInsertClover4-LUMABS-S1 RBD
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNCS-Clover4-LUMABS-EBNA1
Plasmid#183488PurposeTo express the Clover4-LUMABS-EBNA1 sensor for Epstein-barr Virus antibody dection.DepositorInsertClover4-LUMABS-EBNA1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNCS-Clover4-LUMABS-VCA
Plasmid#183489PurposeTo express the Clover4-LUMABS-VCA sensor for Epstein-barr Virus antibody dection.DepositorInsertClover4-LUMABS-VCA
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
2Bc-T MBP_BoMoC(ed)_W403A_6xH
Plasmid#185712PurposeRecombinant B. mori truncated R2 RT expressionDepositorInsertB. mori truncated R2 RT
UseTags6xHis and MBPExpressionBacterialMutationChanged W403 to APromoterT7Available sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLPC-FLAG.HA.HA.Mm.RAP1.I312R
Plasmid#184643PurposeRetroviral expressions of mouse RAP1 I312RDepositorInsertrap1
UseRetroviralTagsHA FLAGExpressionMammalianMutationI312RPromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGB-Co-NF-kB
Plasmid#180206PurposeExpression GAL4-fusion of Co-NF-kB in yeastDepositorInsertCo-NF-kB
UseTagsGAL4 (1-147) fusionExpressionYeastMutationaa 2-1224PromoterAdhIAvailable sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pMC6-spacer
Plasmid#176178Purposeyeast MoClo level-0 part plasmid type 6 containing non-coding DNA spacer for construction of MoClo plasmids without yeast markerDepositorInsert41 bp non-coding DNA spacer from pYTK048
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0776
Plasmid#177066PurposeMoClo Level 1, position 2, transcriptional unit for transient expression of strictosidine synthase (CrSTR) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptideDepositorInsertstrictosidine synthase (CrSTR) from Catharanthus roseus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0774
Plasmid#177064PurposeMoClo Level 1, position 1, transcriptional unit for transient expression of tryptophan decarboxylase (CrTDC) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptideDepositorInserttryptophan decarboxylase (CrTDC) from Catharanthus roseus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0772
Plasmid#177062PurposeMoClo Level 1, position 6, transcriptional unit for transient expression of secologanin synthase (CrSLS1) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptideDepositorInsertsecologanin synthase (CrSLS1) from Catharanthus roseus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0122
Plasmid#177054PurposeMoClo Level 1, position 2, transcriptional unit for transient expression of iridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptideDepositorInsertiridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0118
Plasmid#177050PurposeMoClo Level 1, position 6, transcriptional unit for transient expression of iridoid synthase (CrISY) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptideDepositorInsertiridoid synthase (CrISY) from Catharanthus roseus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0116
Plasmid#177048PurposeMoClo Level 1, position 5, transcriptional unit for transient expression of 8-hydroxygeraniol oxidoreductase (CrGOR) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptideDepositorInsert8-hydroxygeraniol oxidoreductase (CrGOR) from Catharanthus roseus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0114
Plasmid#177046PurposeMoClo Level 1, position 4, transcriptional unit for transient expression of geraniol 8-oxidase (CrG8H) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptideDepositorInsertgeraniol 8-oxidase (CrG8H) from Catharanthus roseus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0112
Plasmid#177044PurposeMoClo Level 1, position 3, transcriptional unit for transient expression of geraniol synthase (CrGES) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptideDepositorInsertgeraniol synthase (CrGES) from Catharanthus roseus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ash1
Plasmid#169711PurposeBacterial Expression of yeast Ash1 truncation (encodes amino acids 420-500)DepositorInsertAsh1 (ASH1 Budding Yeast)
UseTags6xHisExpressionBacterialMutationTruncation including encoding amino acids 420-500PromoterT7Available sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only