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STARR-seq luciferase INF enhancer vector_ORI_ISG15
Plasmid#99322PurposeLuciferase validation vector with ISG15 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activityDepositorInsertenhancer; chr1: 947976 -949995 (ISG15 Human)
UseLuciferase; Starr-seq luciferase validation vectorExpressionMammalianAvailable SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT7NT*-Bri2 113-231 R221E
Plasmid#138134PurposeExpresseds human Bri2 BRICHOS R221E mutant in E. coliDepositorInserthuman Bri2 BRICHOS R221E (ITM2B Human)
TagsNT* tag derived from spider silk proteinsExpressionBacterialPromoterT7Available SinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-YFP-KRAS4B
Plasmid#112718PurposeExpresses YFP-KRAS4B fusion protein used for FRET studies in eukaryotic cells.DepositorInsertYFP-KRAS4B (KRAS Human)
ExpressionMammalianAvailable SinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
px335 Mettl14 sgRNA #2
Plasmid#61514Purposeencodes sgRNA sequence for targeting mouse Mettl14 locus (Cas9-Nickase strategy)DepositorInsertgccgctcccggatctcctgc
UseCRISPRExpressionMammalianAvailable SinceJan. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CFP-KRAS4B
Plasmid#112717PurposeExpresses CFP-KRAS4B fusion protein used for FRET studies in mammalian cells.DepositorAvailable SinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastbac-Flag-BRCA1
Plasmid#223228PurposeInsect expression construct for Flag-tagged BRCA1 protein production and purificationDepositorAvailable SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
px335 Mettl14 sgRNA #1
Plasmid#61515Purposeencodes sgRNA sequence for targeting mouse Mettl14 locus (Cas9-Nickase strategy)DepositorInsertgcggcagctcctagctcagc
UseCRISPRExpressionMammalianAvailable SinceJan. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
STARR-seq luciferase validation vector_ORI_AGAP1
Plasmid#99300PurposeLuciferase validation vector with AGAP1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activityDepositorInsertenhancer; chr2: 236735694-236737193 (AGAP1 Human)
UseLuciferase; Starr-seq luciferase validation vectorExpressionMammalianAvailable SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
STARR-seq luciferase validation vector_mP_AGAP1
Plasmid#99301PurposeLuciferase validation vector with AGAP1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activityDepositorInsertenhancer; chr2: 236735694-236737193 (AGAP1 Human)
UseLuciferase; Starr-seq luciferase validation vectorExpressionMammalianAvailable SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
STARR-seq luciferase validation vector_SCP1_AGAP1
Plasmid#99302PurposeLuciferase validation vector with AGAP1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activityDepositorInsertenhancer; chr2: 236735694-236737193 (AGAP1 Human)
UseLuciferase; Starr-seq luciferase validation vectorExpressionMammalianAvailable SinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
XRE-H2B-eGFP
Plasmid#182294PurposeFluorescent reporter for AhR activityDepositorAvailable SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-RYK-V5/HIS
Plasmid#205610PurposeExpression of human RYK receptor tyrosine kinase in mammalian cellsDepositorAvailable SinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TRKB-V5/HIS
Plasmid#205614PurposeExpression of human TRKB receptor tyrosine kinase in mammalian cellsDepositorAvailable SinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKrox24(MapErk)DsRed
Plasmid#200114PurposeFluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activityDepositorInsertMapErk sequence in minimal promoter
ExpressionMammalianMutationhighly modified sequencePromoter5 copies of designed MapErk sequence, no other pr…Available SinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
LRRK2-WT
Plasmid#17609DepositorAvailable SinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hRXRα
Plasmid#135910PurposeHuman Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector using SV40 PromoterDepositorAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.Flag.HERC2-HECT-C4762A.6xHis
Plasmid#39228DepositorInsertHERC2 HECT Domain (HERC2 Human)
Tags6xHis and FlagExpressionMammalianMutationC4762APromoterT7Available SinceJuly 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-CRY2-mCh-superPLDx100-P2A-CIBN-CAAX
Plasmid#188994PurposeA plasmid for dual expression of CRY2-mCh-superPLDx100 (an engineered PLD mutant with 100 times higher activity than the wild-type) and plasma membrane-tagged CIBNDepositorInsertPLD
TagsCAAX, CIBN, CRY2, P2A, and mCherryExpressionMammalianMutationK57R, A59V, K109R, P245A, V264I, G328S, G381V, G4…PromoterCMVAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSOMA-T679A-NLS
Plasmid#118938PurposeUsed to express the T679A mutant form of the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thalianaDepositorInsertSOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Localization Signal
ExpressionPlantAvailable SinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Akt-FoxO3a-KTR-EGFP
Plasmid#125131PurposeFluorescent reporter for Akt activityDepositorInsertFOXO2 (FOXO3 Human)
TagsEnhanced green fluorescent protein (EGFP)ExpressionMammalianMutationFoxO3A 1-402 amino acidPromoterCAG promoterAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_V5-TWIST1
Plasmid#215030PurposeMammalian expression of human TWIST1DepositorInserttwist family bHLH transcription factor 1 (TWIST1 Human)
TagsV5ExpressionMammalianPromoterCMVAvailable SinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKrox24(2xD-E_inD)Luc
Plasmid#200109PurposeLuciferase reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activityDepositorInsertmodified EGR1 promoter
ExpressionMammalianMutationhighly modified sequencePromoter2 copies of D-E element in front of EGR1 promoter…Available SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TEK-V5/HIS
Plasmid#205611PurposeExpression of human TEK receptor tyrosine kinase in mammalian cellsDepositorAvailable SinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
DDX41-GFP
Plasmid#175494PurposeTotal RNA was isolated from TF-1 cells and converted to cDNA using SuperScript III (Invitrogen). The full DDX41 coding sequence was cloned into pcDNA3.1 (Invitrogen) with an EGFP c-terminal insert.DepositorAvailable SinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only
