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pRSET Nucleolin

Plasmid

#13037

Depositor

Peter Newburger

Publication

Wu et al J Cell Biochem. 2000 Apr . 77(3):507-16.

Insert

Nucleolin (NCL Human)

Tags

6xHis

Expression

Bacterial

Available since

Oct. 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCSII-EF/mt-(n1)StayGold

Plasmid

#185823

Purpose

A soluble photostable fluorescent marker of the mitochondrial matrix. The StayGold gene has codon optimization for mammalian expression.

Depositor

Atsushi Miyawaki

Publication

Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

Insert

mt-(n1)StayGold

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Lenti-human-TF-gRNA-puromycin

Pooled library

#162275

Purpose

Knockout library targeting a set of human transcription factors for loss-of-function screening.

Depositor

Chunliang Li

Publication

Zhang et al Elife. 2020 Oct 1;9. pii: 57858. doi: 10

Has service

Lentiviral Prep

Species

Homo sapiens

Use

Lentiviral

Available since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pLX208 CMV sTurboID (C)-HA-FRB-ERM

Plasmid

#153007

Purpose

Expresses split-TurboID C-terminal fragment targeted to endoplasmic reticulum membrane

Depositor

Alice Ting

Publication

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

sTurboID (C) (G74)-ERM (Cb5)

Use

Lentiviral

Tags

FRB and HA

Expression

Mammalian

Promoter

CMV

Available since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCMV-T7-SpRY-P2A-EGFP (RTW4830)

Plasmid

#139989

Purpose

CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

Depositor

Benjamin Kleinstiver

Publication

Walton et al Science. 2020 Apr 17;368(6488):290-296.

Insert

human codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

Promoter

CMV and T7

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pHelper_ShCAST_sgRNA

Plasmid

#127921

Purpose

Expresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).

Depositor

Feng Zhang

Publication

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pAAV.Syn.NES-jRCaMP1b.WPRE.SV40 (AAV1)

Viral prep

#100851-AAV1

Purpose

Ready-to-use AAV1 particles produced from pAAV.Syn.NES-jRCaMP1b.WPRE.SV40 (#100851). In addition to the viral particles, you will also receive purified pAAV.Syn.NES-jRCaMP1b.WPRE.SV40 plasmid DNA. Syn-driven jRCaMP1b calcium sensor. These AAV preparations are suitable purity for injection into animals.

Depositor

Douglas Kim, GENIE Project

Publication

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Promoter

Syn

Available since

April 13, 2018

Availability

Academic Institutions and Nonprofits only
hCRISPRa-v2, genome-wide, 10 sgRNAs/gene

Pooled library

#1000000091

Purpose

Genome-wide CRISPR gRNA pooled library for activation of human genes

Depositor

Jonathan Weissman

Available since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only
pET28a-MSP1D1delltaH5

Plasmid

#71714

Purpose

Membrane scaffold protein (MSP), helix 5 deletion

Depositor

Gerhard Wagner

Publication

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Tags

6xHis and TEV site

Expression

Bacterial

Available since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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phage UBC NLS-HA-2XMCP-tagRFPt

Plasmid

#64541

Purpose

Used to label reporter mRNAs. Lentiviral expression of the MCP protein fused to tagRFP.

Depositor

Jeffrey Chao

Publication

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

MCP

Use

Lentiviral

Tags

HA, NLS, and tagRFP-T

Expression

Mammalian

Promoter

human ubiquitin C promoter

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pCAG-ChrimsonR-tdT

Plasmid

#59169

Purpose

Expression vector for ChrimsonR-tdTomato

Depositor

Edward Boyden

Publication

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR-tdTomato

Tags

tdTomato

Expression

Mammalian

Mutation

K176R

Promoter

CAG

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAAV-CaMKIIa-hM3D(Gq)-mCherry

Plasmid

#50476

Purpose

Gq-coupled hM3D DREADD fused with mCherry under the control of CaMKIIa promoter

Depositor

Bryan Roth

Has service

AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

hM3D(Gq)-mCherry (CHRM3 Human)

Use

AAV

Tags

mCherry

Mutation

See supplemental documents for DREADD mutations

Promoter

CaMKIIa

Available since

April 16, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

41 Articles

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pICH86966::AtU6p::sgRNA_PDS

Plasmid

#46966

Purpose

Expresses an sgRNA targeting the PDS gene in Nicotiana benthamiana from the Arabidopsis U6 promoter

Depositor

Sophien Kamoun

Publication

Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

Insert

AtU6p::sgRNA_PDS

Use

CRISPR; Plant expression

Available since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

26 Articles

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pEGFP VAMP2

Plasmid

#42308

Depositor

Thierry Galli

Publication

Martinez-Arca et al Proc Natl Acad Sci U S A. 2003 Jul 22;10

Insert

VAMP 2

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

March 12, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pCI-ASC-HA

Plasmid

#41553

Depositor

Kate Fitzgerald

Publication

Hornung et al Nature. 2009 Mar 26. 458(7237):514-8.

Insert

ASC (PYCARD Human)

Tags

HA

Expression

Mammalian

Promoter

CMV immediate-early enhancer/promoter

Available since

Dec. 20, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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L13-Arl13bGFP

Plasmid

#40879

Purpose

3rd generation transfer vector

Depositor

Tamara Caspary

Publication

Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

Insert

Arl13b (Arl13b Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pT3-EF1a-c-Met

Plasmid

#31784

Depositor

Xin Chen

Publication

Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

Insert

Met (MET Human)

Expression

Mammalian

Promoter

EF1alpha

Available since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pLenti PGK BirA Hygro (w874-1)

Plasmid

#29649

Depositor

Eric Campeau

Insert

BirA

Use

Lentiviral

Expression

Mammalian

Available since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pmCherry-CIBN-CreC

Plasmid

#26889

Purpose

Expresses C-terminal fragment of Cre fused to CIBN for blue light-induced activation of DNA recombination when used with CRY2-CreN. Also expresses mCherry.

Depositor

Chandra Tucker

Publication

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CIBN-CreC

Expression

Mammalian

Mutation

aa 1-170 of CIB1, aa 106-343 of Cre

Promoter

CMV

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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MSCV-Cre

Plasmid

#24064

Purpose

Retroviral coexpression of Cre and EGFP

Depositor

Dan Littman

Insert

Cre

Use

Cre/Lox and Retroviral

Tags

EGFP

Expression

Mammalian

Promoter

MESV 5' LTR

Available since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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