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NID1-bio-His

Plasmid

#52068

Purpose

Expresses full-length Nidogen-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

NID1 (NID1 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG Cas9-2A-Citrine

Plasmid

#92358

Purpose

CAG-driven ubiquitous expression of Cas9. Contains 2A-Citrine reporter. For CRISPR mediated gene knockouts in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

Cas9 2A Citrine

Expression

Mammalian

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pHsp83-NLS-HA-2xMCP-2xTagRFP-T

Plasmid

#71242

Purpose

Used to label reporter mRNAs. Drosophila expression of the MCP protein fused to TagRFP-T.

Depositor

Anne Ephrussi

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

NLS-2xMCP-2xTagRFP-T

Use

P element-mediated germline transformation

Tags

TagRFP-T

Expression

Insect

Promoter

Hsp83

Available Since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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HSF1-GFPN3

Plasmid

#32538

Depositor

Stuart Calderwood

Article

Wang et al Mol Cell Biol. 2003 Sep;23(17):6013-26.

Insert

HSF1 (HSF1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

see depositor comments below

Promoter

CMV

Available Since

Oct. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pDEST-mScarlet-SH3PXD2A

Plasmid

#215534

Purpose

Expression vector with Tks5 tagged with N-terminal mScarlet

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

SH3PXD2A (SH3PXD2A Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pLR5-CAG-hMCP-mScarlet-I-NLS

Plasmid

#174878

Purpose

PiggyBac transposon vector for expression of MCP-mScarlet-I-NLS

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

MCP-mScarlet-I-NLS

Use

Piggybac vector

Expression

Mammalian

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-GASC1

Plasmid

#24214

Purpose

Mammalian expression of human JMJD2C with HA tag

Depositor

Kristian Helin

Article

Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

Insert

GASC1 (KDM4C Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pLEX303-GFP

Plasmid

#162032

Purpose

Tagged vector control

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Type

Empty backbone

Use

Lentiviral

Tags

GFP

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-PELP1

Plasmid

#65208

Purpose

Full length mammalian expression vector for hPELP1

Depositor

Ratna Vadlamudi

Article

Nagpal et al Mol Cancer Res. 2008 May;6(5):851-61. do

Insert

PELP1 (PELP1 Human)

Tags

AcGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only
pRevTRE MKL1

Plasmid

#19846

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 (MRTFA Human)

Use

Retroviral

Tags

3xFLAG

Available Since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only
pDEST26-USP47

Plasmid

#64326

Purpose

Mammalian expression and purification of USP47.

Depositor

Grigory Dianov

Article

Parsons et al Mol Cell. 2011 Mar 4;41(5):609-15. doi:

Insert

USP47 (USP47 Human)

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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pUCHR-osTIR1-IRES-GFP

Plasmid

#110661

Purpose

Lentiviral vector for stable expression of osTIR1 and selection with GFP

Depositor

Dmitriy Mazurov

Article

Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

Insert

osTIR1

Use

Lentiviral

Promoter

CMV

Available Since

March 1, 2019

Availability

Academic Institutions and Nonprofits only

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V246 pCEP 4HA B56beta

Plasmid

#14533

Depositor

David Virshup

Article

Seeling et al Science. 1999 Mar 26. 283(5410):2089-91.

Insert

PPP2R5B B56 beta regulatory subunit of PP2A (PPP2R5B Human)

Tags

4 HA

Expression

Mammalian

Mutation

I346V relative to GenBank ID L42374

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

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pQE60 Q58L wgMDH

Plasmid

#204246

Purpose

Bacterial expression of Q58L wgMDH with IPTG induction - Subunit interface mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

Q58L

Promoter

T5

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH_Str-KDEL_ManII-SBP-mCherry

Plasmid

#65259

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin-KDEL and truncated Mannosidase II

Use

Lentiviral

Tags

Streptavidin Binding Protein (SBP) and mCherry

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pMRXIB-WIPI2b-mRuby3

Plasmid

#221749

Purpose

Stable expression of WIPI2b-mRuby3 in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

WIPI2b (WIPI2 Human)

Use

Retroviral

Tags

mRuby3

Expression

Mammalian

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-ChR2-TdTomato (Cre-OFF)

Plasmid

#166610

Purpose

Encodes Cre-inactivated ChR2-TdTomato under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

Insert

ChR2-TdTomato

Use

AAV

Mutation

H134R

Promoter

tetracycline response element

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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myc-Rictor

Plasmid

#1860

Purpose

Please see Addgene item #11367 for an updated version of this plasmid.

Depositor

David Sabatini

Article

Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

Insert

Rictor (RICTOR Human)

Tags

Myc

Expression

Mammalian

Available Since

June 20, 2005

Availability

Academic Institutions and Nonprofits only

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pSLQ2814 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SpdCas9

Plasmid

#84247

Purpose

Expresses direct fusion VPR-Sp dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

VPR-tagBFP-Sp dCas9
Puro

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), HA tag, VPR, and tagBFP

Expression

Mammalian

Promoter

CAG and PGK

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA6-IgK-hFRa(23-234)C11S-th-Fc

Plasmid

#177841

Purpose

Mammalian Expression of Folate Receptor

Depositor

Karsten Melcher

Article

Chen et al Nature. 2013 Aug 22;500(7463):486-9. doi

Insert

Folate receptor alpha (FOLR1 Human)

Expression

Mammalian

Mutation

all 11 C mutated to S

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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