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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,310 results for: LEA

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Showing: 2841 - 2860 of 12310 results

  1. pET-TP1107(Q15TAG)

    Plasmid
    #247462
    Purpose
    Bacterial expression of anti-IgG VHH clone TP1107 with amber stop codon at Q15 for unnatural amino acid incorporation
    Depositor
    Angus Johnston
    Publication
    Chen et al Nat Nanotechnol. 2025 Sep;20(9):1273-128
    Insert
    TP1107 Q15TAG
    Tags
    His6 and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Original Q15 codon (CAA) was modified to amber st…
    Promoter
    T7
    Available since
    Dec. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    247462-plasmid-map-sequence-id-494410

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  2. pK263.CAG-loxP-stop-loxP-NLSturboRFP-ires-tTA-WPRE

    Plasmid
    #89587
    Purpose
    For nucleus-specific turboRFP labeling in single cells, pK263 should be used with pK031.
    Depositor
    Takuji Iwasato
    Publication
    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nuclear localization signal (nls) turboRFP
    Expression
    Mammalian
    Available since
    June 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    89587-plasmid-map-sequence-id-180320

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  4. pMesh1*

    Plasmid
    #175591
    Purpose
    Lac inducible, AMP Resistant, fluor labelled (CFP) gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.
    Depositor
    Greg Bokinsky
    Publication
    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.
    Insert
    Mesh1 (Mesh1 Fly)
    Tags
    Fused with a flexible GS linker to cerulean
    Expression
    Bacterial
    Mutation
    Codon optimized for translation in E.coli. AGGAGG…
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    175591-plasmid-map-sequence-id-348365

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  7. SpRY-ABE8e(V106W)

    Plasmid
    #198553
    Purpose
    Expresses human codon-optimized SpRY-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpRY-ABE8e(V106W)-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpRY-ABE8e(V106W)
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198553-plasmid-map-sequence-id-388905

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  8. SpRY-YE1-BE4max

    Plasmid
    #198554
    Purpose
    Expresses human codon-optimized SpRY-YE1-BE4max and blasticidin resistance: EFS promoter-SpRY-YE1-BE4max-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpRY-YE1-BE4max
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198554-plasmid-map-sequence-id-388916

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  11. pET28-AviTag-DogTag-MBP

    Plasmid
    #171773
    Purpose
    Expresses AviTag-DogTag-Maltose binding protein in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.
    Depositor
    Mark Howarth
    Publication
    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-
    Insert
    AviTag-DogTag-MBP
    Tags
    His6 and Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Peptide tag added to N-terminus of MBP via a GSGE…
    Promoter
    T7
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171773-plasmid-map-sequence-id-338517

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  12. DsRed2-Nucleus-4

    Plasmid
    #55839
    Purpose
    Localization: Nucleus, Excitation: 558, Emission: 583
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SV40 T-Antigen Nuclear Localization Signal (NLS)
    Tags
    DsRed2-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 12, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. pCMV-P10HA

    Plasmid
    #1345
    Depositor
    Li-Huei Tsai
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    P10 (CDK5R1 Human)
    Tags
    HA
    Expression
    Mammalian
    Available since
    April 13, 2006
    Availability
    Academic Institutions and Nonprofits only

  16. pCAG-HA-NLGN3 R451C

    Plasmid
    #59319
    Purpose
    Expression plasmid of HA-tagged human Neuroligin 3 harboring an R451C mutation
    Depositor
    Peter Scheiffele
    Publication
    Chih et al Hum Mol Genet. 2004 Jul 15;13(14):1471-7
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLGN3 R451C (NLGN3 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Arginine 451 to Cysteine (R451C mutation)
    Promoter
    CAG
    Available since
    June 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    59319-plasmid-map-sequence-id-107092

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  17. SMYD3

    Plasmid
    #32048
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SMYD3 (SMYD3 Human)
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by

  18. pEMS1172

    Plasmid
    #29301
    Purpose
    Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).
    Depositor
    Elizabeth Simpson
    Publication
    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ple151 (OLIG1 Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    EGFP-NLS
    Available since
    Jan. 6, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    29301-plasmid-map-sequence-id-13716

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  19. pEMS1052

    Plasmid
    #29004
    Purpose
    Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested
    Depositor
    Elizabeth Simpson
    Publication
    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ple112 (HCRT Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    Cre-EGFP-NLS
    Available since
    Aug. 4, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    29004-plasmid-map-sequence-id-391177

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  20. pAAV-Ef1a-fDIO EYFP (AAV Retrograde)

    Viral prep
    #55641-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-Ef1a-fDIO EYFP (#55641). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO EYFP plasmid DNA. EF1a-driven, Flp-dependent expression of EYFP. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Publication
    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:
    Promoter
    EF1a
    Tags
    EYFP (Flp-dependent)
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 2841 - 2860 of 12310 results