We narrowed to 152,678 results for: des

1. pINDUCER dCas9-TET1CD

   Plasmid

   #101921

   Purpose

   The dCas9-TET1CD fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948).

   Depositor

   Danwei Huangfu

   Article

   Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

   Insert

   dCas9-TET1CD
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. PRPF19

   Plasmid

   #155847

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   PRPF19 (PRPF19 Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ZFP36L2

   Plasmid

   #156149

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   ZFP36L2 (ZFP36L2 Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pX330 PLIN2

   Plasmid

   #202196

   Purpose

   pX330 backbone expressing sgRNA to edit the C-terminus of human PLIN2

   Depositor

   James Olzmann

   Article

   Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

   Insert

   PLIN2 (PLIN2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. ZNF217_pLX307

   Plasmid

   #98384

   Purpose

   Lentiviral expression of ZNF217

   Depositor

   Sefi Rosenbluh, William Hahn

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   ZNF217 (ZNF217 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   E1Fa

   Available since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGART-2XStrep

   Plasmid

   #125681

   Purpose

   expresses human GART protein with a C-terminal 2XStrep affinity tag

   Depositor

   Stephen Benkovic

   Article

   Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

   Insert

   GART (GART Human)
   

   Use

   Tags

   2X Strep-Tag II

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. non-targeting control gRNA (BRDN0001146145)

   Plasmid

   #80198

   Purpose

   3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   Non-targeting control gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mCh-KIF5B*-strep

   Plasmid

   #120164

   Purpose

   Expresses a chimera of mCherry (fluorescent tag), a KIF5B-based anterograde motor and streptavidin

   Depositor

   Juan Bonifacino

   Article

   Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

   Insert

   kinesin family member 5B (Kif5b Mouse)
   

   Use

   Tags

   Streptavidin and mCherry

   Expression

   Mammalian

   Mutation

   Truncated KIF5B: 1-555 aa

   Promoter

   CMV

   Available since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. TOM20*-SBP-GFP

   Plasmid

   #120173

   Purpose

   Expresses a chimera of the mitochondria-targeting signal of TOM20, SBP tag and GFP (fluorescent tag)

   Depositor

   Juan Bonifacino

   Article

   Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

   Insert

   Mitochondrial import receptor subunit TOM20 homolog (TOMM20 Human)
   

   Use

   Tags

   SBP and eGFP

   Expression

   Mammalian

   Mutation

   Truncated TOM20: 1-30 aa

   Promoter

   CMV

   Available since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. NP-11-64-1

    Plasmid

    #234359

    Purpose

    Expressing rubisco and prk in Δrpi

    Depositor

    David Savage

    Article

    Prywes et al Nature. 2025 Jan 22. doi: 10.1038/s41586

    Insert

    R.rubrum rubisco
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. 1518_pAAV-U6-SA-eGFP-gRNA-HLP-SACas9-HA-OLLAS-spA

    Plasmid

    #109316

    Purpose

    Plasmid for liver-specific expression of AAV SaCas9 with a gRNA against eGFP

    Depositor

    William Lagor

    Article

    Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

    Insert

    eGFP gRNA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSCV-mir-17-92

    Plasmid

    #64100

    Purpose

    expresses mir-17-92

    Depositor

    Lin He

    Article

    Olive et al Elife. 2013 Oct 15;2:e00822. doi: 10.755

    Insert

    miR-17-92 (MIR17HG Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL3-IRES-miR34a-P2

    Plasmid

    #64786

    Purpose

    Luciferase expression driven by miR-34a promoter P2

    Depositor

    Joshua Mendell

    Article

    Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

    Insert

    miR-34a promoter P2 (MIR34A Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SF3A2

    Plasmid

    #156001

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    SF3A2 (SF3A2 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUG-natNT2

    Plasmid

    #110922

    Purpose

    expression of nat gene conferring resistance to nourseothricin for gene deletion in yeast

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Insert

    nat
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    AgTEF1

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PRPF3

    Plasmid

    #155848

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    PRPF3 (PRPF3 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PRPF4

    Plasmid

    #155850

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    PRPF4 (PRPF4 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. STK11 gRNA (BRDN0001146880)

    Plasmid

    #75912

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human STK11

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    STK11 (STK11 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-FLEX-hGTB

    Plasmid

    #26196

    Depositor

    Edward Callaway

    Article

    Wall et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

    Insert

    Tet transactivator, enhanced green fluorescent protein, TVA, rabies B19 glycoprotein
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Tags

    Expression

    Mutation

    F2A has 2A cleavage site deleted, producing htTA-…

    Promoter

    Available since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSEVA331Bb

    Plasmid

    #78269

    Purpose

    pSEVA331 with Biobrick multiple cloning site

    Depositor

    Tom Ellis

    Article

    Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHLmMBP-11

    Plasmid

    #72349

    Purpose

    Mammalian expression of secreted mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

    Depositor

    Luca Jovine

    Article

    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

    Type

    Empty backbone

    Use

    Tags

    6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV enhancer + chicken beta-actin promoter

    Available since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3-FLAG-HA-CAD S1859A

    Plasmid

    #46239

    Purpose

    FLAG-HA-CAD with S1859 phosphorylation site mutated to alanine

    Depositor

    Brendan Manning

    Article

    Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. d

    Insert

    carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Serine 1859 changed to Alanine (S1859A)

    Promoter

    cmv

    Available since

    Nov. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

23. pScalps_puro_mIL-2Rβ

    Plasmid

    #59916

    Purpose

    Expresses mouse interleukin-2 receptor Beta chain

    Depositor

    Silvia Monticelli

    Article

    Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

    Insert

    interleukin 2 receptor beta (Il2rb Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

24. pHR_Gal4UAS_4D5-Low_CAR-mCherry_pGK_4D5-Low_SynNotch_RHL183

    Plasmid

    #164827

    Purpose

    Lentiviral vector for Gal4 inducible AntiHER2 4D5-Low CAR-mCherry and constitutive expression of antiHER2 4D5-Low Gal4VP64 Synthetic Notch

    Depositor

    Wendell Lim

    Article

    Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

    Inserts

    AntiHER2 4D5-Low Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
    AntiHER2 4D5-Low Gal4VP64 synthetic Notch receptor
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    miniCMV and pGK

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pScalps_puro_mIL-2Rα

    Plasmid

    #59917

    Purpose

    Expresses mouse interleukin-2 receptor alpha chain

    Depositor

    Silvia Monticelli

    Article

    Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

    Insert

    interleukin 2 receptor alpha (Il2ra Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

26. TBK1 gRNA (BRDN0001148607)

    Plasmid

    #76363

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TBK1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TBK1 (TBK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. TRIM28 gRNA (BRDN0001162487)

    Plasmid

    #76139

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRIM28

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRIM28 (TRIM28 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHR_Gal4UAS_4D5-Low_CAR-mCherry_pGK_4D5-Medium_SynNotch_RHL144

    Plasmid

    #164828

    Purpose

    Lentiviral vector for Gal4 inducible AntiHER2 4D5-Low CAR-mCherry and constitutive expression of antiHER2 4D5-Medium Gal4VP64 Synthetic Notch

    Depositor

    Wendell Lim

    Article

    Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

    Inserts

    AntiHER2 4D5-Low Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
    AntiHER2 4D5-Medium Gal4VP64 synthetic Notch receptor
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    miniCMV and pGK

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAF92

    Plasmid

    #135929

    Purpose

    All-in-one Sleeping Beauty vector for Expression for Tasmanian devil mCherry-TNFSF9 (type II transmembrane) fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFSF9).

    Depositor

    Andrew S. Flies

    Article

    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

    Insert

    mCherry fused to TNFSF9
    

    Use

    Transposon

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTET2-RlucCP-3MS2

    Plasmid

    #198354

    Purpose

    Part of Renilla reporter system, encodes Renilla luciferase with MS2 stem loops in the 3'UTR

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    Renilla Luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTET2

    Available since

    April 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pJEP316-pAAV-U6SaCas9gRNA(SapI)-pA Empty Cassette

    Plasmid

    #113693

    Purpose

    U6 driven SaCas9 gRNA expression cassette without a gRNA. SapI can be used to clone in gRNAs.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    SaCas9 gRNA Cassete
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Str-STIM1-NN_neomycin

    Plasmid

    #65311

    Purpose

    Luminal ER hook only (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    STIM1 mutated for binding motif to microtubule (STIM1 Human)
    

    Use

    Tags

    streptavidin

    Expression

    Mammalian

    Mutation

    2 amino acids of STIM1 (IP from SxIP motif) were …

    Promoter

    CMV

    Available since

    June 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. TVBB C-term-mScarlet-Blast

    Plasmid

    #169221

    Purpose

    Targeting vector backbone to support a knock-in of Linker-mScarlet-P2A-Blast at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mScarlet-P2A-Blast
    

    Use

    Targeting vector backbone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. NSUN2

    Plasmid

    #155793

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    NSUN2 (NSUN2 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-Puro_siKO

    Plasmid

    #86696

    Purpose

    Tetracycline inducible expression of guide RNA targeted to AAVS1 locus

    Depositor

    Ludovic Vallier

    Article

    Bertero et al Development. 2016 Dec 1;143(23):4405-441

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1 TO

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAd-Track Flag HA PGC-1 alpha

    Plasmid

    #14426

    Depositor

    Pere Puigserver

    Article

    Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

    Insert

    PGC-1 alpha (Ppargc1a Mouse)
    

    Use

    Adenoviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Last four amino acids of PGC-1a removed to make t…

    Promoter

    Available since

    Feb. 23, 2007

    Availability

    Academic Institutions and Nonprofits only

37. TRIM28 gRNA (BRDN0001145369)

    Plasmid

    #76141

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRIM28

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRIM28 (TRIM28 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. SR140

    Plasmid

    #156037

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    SR140 (U2SURP Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. TVBB C-term-mNeongreen

    Plasmid

    #169227

    Purpose

    Targeting vector backbone to support a knock-in of Linker-mNeongreen at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mNeongreen
    

    Use

    Targeting vector backbone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTA-Mob 2.0 Tp

    Plasmid

    #188619

    Purpose

    Mutated pTA-Mob 2.0 Backbone in TraJ Promoter Region

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Conjugation helper plasmid

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    May 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge