We narrowed to 44,063 results for: tro
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Plasmid#177019PurposeLevel 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTTDepositorInsert1-deoxy-D-xylulose 5-phosphate synthase
UseSynthetic BiologyMutationtransit peptide removed, amplified from cDNA, con…Available SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_Camk2a sgRNA / hSpCas9
Plasmid#172839PurposeMammalian expression of a sgRNA targeting the intron 17 (last intron) of Camk2a (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 17 of Camk2a under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
THI ONLY pALD4 T-
Plasmid#161497PurposeNon activation and non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T-_gRNA_MS2DepositorInsertsdCAS9
MS2 (non activation control NAC)
gRNA (Non targeting control NTC)
ExpressionYeastPromoterpALD4, pPOR1, and pTEF2Available SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
UAS-u(XXL)Cas9
Plasmid#127387PurposeExpresses Cas9 at very low levelDepositorInsertuORF(XXL)-Cas9
UseCRISPRExpressionInsectAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAA2
Plasmid#154162PurposeFirefly luciferase under the control of 4 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.DepositorInsertFirefly luciferase
UseLuciferaseMutation16 wt NF-κB sites were replaced with Rel1A-specif…Available SinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAA1
Plasmid#154161PurposeFirefly luciferase under the control of 3 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.DepositorInsertFirefly luciferase
UseLuciferaseMutation12 wt NF-κB sites were replaced with Rel1A-specif…Available SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM62
Plasmid#154160PurposeFirefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, with 4 Rel2-specific (VII) NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.DepositorInsertFirefly luciferase
UseLuciferaseMutation4 wt NF-κB sites were replaced with Rel2-specific…Available SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM59
Plasmid#154158PurposeFirefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, a TATA box and Kozak sequence of the Metchnikowin gene and additional NF-κB site.DepositorInsertFirefly luciferase
UseLuciferasePromoterEnhancer for the Drosophila melanogaster Metchnik…Available SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM52
Plasmid#154155PurposeFirefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter with 8 copies of the Aedes aegypti Rel2 NF-κB siteDepositorInsertFirefly luciferase
UseLuciferasePromoterDrosophila melanogaster minimal Hsp70 promoter wi…Available SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM51
Plasmid#154154PurposeFirefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter with 8 copies of the Aedes aegypti Rel1A NF-κB siteDepositorInsertFirefly luciferase
UseLuciferasePromoterDrosophila melanogaster minimal Hsp70 promoter wi…Available SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetDBl-CLIP-HsSAS6
Plasmid#136839PurposeMammalian expression of the centrosomal protein SAS6 N-terminally fused to CLIP-tagDepositorInsertCLIP-SAS6 (SASS6 Human, Synthetic)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-Sfi1
Plasmid#136842PurposeMammalian expression of the centrosomal protein hSfi1 N-terminally fused to CLIP-tagDepositorInsertCLIP-hSfi1 (SFI1 Human, Synthetic)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-HsSAS6
Plasmid#136838PurposeMammalian expression of the centrosomal protein SAS6 N-terminally fused to CLIP-tagDepositorInsertCLIP-SAS6 (SASS6 Human, Synthetic)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/AAA-nLuc-3XFLAG
Plasmid#127306PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from AAA start codon (Negative control plasmid)DepositorInsertnanoLuciferase
Tags3xFLAGExpressionMammalianPromoterCMVAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/GGG-stop-nLuc-3XFLAG
Plasmid#127311PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from negative control GGG start codon, but translation terminated immediately by stop codonDepositorInsertnanoLuciferase
Tags3xFLAGExpressionMammalianPromoterCMVAvailable SinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/AAA-stop-nLuc-3XFLAG
Plasmid#127310PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from negative control AAA start codon, but translation terminated immediately by stop codonDepositorInsertnanoLuciferase
Tags3xFLAGExpressionMammalianPromoterCMVAvailable SinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/GGG-nLuc-3XFLAG
Plasmid#127307PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from GGG start codon (Negative control plasmid)DepositorInsertnanoLuciferase
Tags3xFLAGExpressionMammalianPromoterCMVAvailable SinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHW564 (Pmyo-2::cGAL-N(C1A)::let-858 3'UTR)
Plasmid#107739Purposemutated cGAL-N (C1A) driver under the control of C. elegans myo-2 promoterDepositorInsertNLS::cGAL(DBD)::gp41-1-N-intein(C1A)
ExpressionWormMutationchange the first cysteine of gp41-1 N-intein to a…PromoterC. elegans myo-2 promoterAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
p.UTA.2.0-GL-2-3 perfect copies
Plasmid#82441PurposeDual Fluorescent endogenous miRNA sensor Control insert. Three Perfect copies of complementary region of a siRNA control targeting firefly luciferase encoded by the pGL2DepositorInsertLuciferase
MutationThe complementary region for GL2 siRNA control wa…Available SinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 PAK5 P50L
Plasmid#60533PurposeDonor vector for human Pak5 P50L mutantDepositorAvailable SinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 PAK5 P76T
Plasmid#60534PurposeDonor vector for human Pak5 P76T mutantDepositorAvailable SinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 PAK5 S312C
Plasmid#60535PurposeDonor vector for human Pak5 S312C mutantDepositorAvailable SinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 PAK5 V463L
Plasmid#60536PurposeDonor vector for human Pak5 V463L mutantDepositorAvailable SinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 PAK5 V593I
Plasmid#60539PurposeDonor vector for human Pak5 V593I mutantDepositorAvailable SinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMA3411
Plasmid#46877PurposeA retroviral vector for the constitutive expression of soluble guanylate cyclase1 beta3 double mutant (E473K and C541D)DepositorInsertsoluble guanylate cyclase1 beta 3 (Gucy1b3 Rat)
UseRetroviralMutationE473K, C541DPromoterLTRAvailable SinceAug. 14, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSMP-MBD4_3
Plasmid#36376DepositorAvailable SinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSMP-MBD4_2
Plasmid#36375DepositorAvailable SinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
UAS-u(XS)Cas9
Plasmid#127382PurposeExpresses Cas9 at very high levelDepositorInsertuORF-Cas9
UseCRISPRExpressionInsectAvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3-hEPO
Plasmid#50436Purposeexpress human erythropoietin using lentiviral delivery systemDepositorAvailable SinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTN4
Plasmid#174212PurposeDonor plasmid for introducing Peft-3::AtTIR1(F79G)::mRuby at the ttTi5605 locusDepositorAvailable SinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF3549
Plasmid#145025PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens. This is the control vector for the collection.DepositorInsertGFP
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSI-Check2-hRluc-NFkB-firefly
Plasmid#106979PurposeLuciferase reporter with internal control for NFkB signal.DepositorInsertNFKB recognition sites (NFKB1 Human)
UseLuciferaseAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFORF3550
Plasmid#145026PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens. This is the control vector for the collection.DepositorInsertmCherry
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
tetO.Nfib.Hygro
Plasmid#117271PurposeExpresses Nfib and hygromycin resistance gene under control of tetO sequence.DepositorInsertNfib (Nfib Mouse)
UseLentiviralTagsIRES and hygromycin resistant cassette (C termina…ExpressionMammalianPromotertet-onAvailable SinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
tetO.Sox9.Puro
Plasmid#117269PurposeExpresses Sox9 and puromycin resistance gene under control of tetO sequence.DepositorInsertSox9 (Sox9 Mouse)
UseLentiviralTagsT2A and puromycin resistant cassette (C terminal …ExpressionMammalianPromotertet-onAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pB PUb-integrase
Plasmid#183966PurposepiggyBac plasmid for insect transformation, expressing PhiC31 integrase under control of the Aedes aegypti PUb promoter, GFP fluorescent markerDepositorInsertPhiC31 integrase
UsePiggybac insect transgenesis destination vectorPromoterAedes aegypti PolyubiquitinAvailable SinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSP6-RNAP
Plasmid#48143PurposeShuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage SP6 under control of the xylose inducible promoter PxylADepositorInsertrna polymerase SP6
UseSynthetic BiologyExpressionBacterialMutationV314A (numbering based on NCBI Sequence ID: NP_85…Available SinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-IV-GFPL10
Plasmid#98704PurposeCre-dependently express GFPL10 fusion using Introvert SwitchDepositorInsertGFPL10
UseAAV and Cre/LoxTagsGFPExpressionMammalianMutationL10: 2609 C->T to remove EcoRI sitePromoterEF1aAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSCV(gfp)U6sgRNA5(BbsI)-PGKpuroBFP
Plasmid#102798PurposeRetrovirus for delivery of one sgRNA (self-targeting) - GFP-targeting control plasmid contains GFP instead of PuroRDepositorInsertssgRNA
EGFP
UseCRISPR and RetroviralExpressionMammalianMutationH231LAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLM-mCerulean-cMyc
Plasmid#23244DepositorInsertmCerulean-2A-MYC (MYC Human, synthetic fluorescent protein)
UseLentiviralAvailable SinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only