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We narrowed to 34,841 results for: cas

Showing: 2901 - 2920 of 34841 results

  5. dCas9-NLS-gs10-cLYN268

    Plasmid
    #188259
    Purpose
    Plasmid ensures constitutive expression of the C-terminal fragment of split LYN kinase domain (aa 239-267), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus
    Depositor
    Roman Jerala
    Article
    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038
    Insert
    cLYN268 (LYN Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    188259-plasmid-map-sequence-id-374580

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  6. dCas9-NLS-gs10-cERb447

    Plasmid
    #188257
    Purpose
    Plasmid ensures constitutive expression of the C-terminal fragment of split ERbeta (aa 447-501), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus
    Depositor
    Roman Jerala
    Article
    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038
    Insert
    cERb447 (ESR1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    188257-plasmid-map-sequence-id-374591

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  7. dCas9-NLS-gs10-cTRb413

    Plasmid
    #188261
    Purpose
    Plasmid ensures constitutive expression of the C-terminal fragment of split TRbeta (aa 413-460), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus
    Depositor
    Roman Jerala
    Article
    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038
    Insert
    cTRb413 (TXNRD2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    188261-plasmid-map-sequence-id-374572

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  9. pnos-Fok1:dCas9-nos

    Plasmid
    #62210
    Purpose
    Expresses Fok1:dCas9 under control of nanos promoter and 3'UTR. For germ line restricted high specificity genome engineering in Drosophila melanogaster.
    Depositor
    Simon Bullock
    Insert
    Fok1-dCas9
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    nanos
    Available since
    March 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    62210-plasmid-map-sequence-id-101869

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  17. pRW004-SM-destination-proUBQ10-Cas9

    Plasmid
    #104438
    Purpose
    Destination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;
    Depositor
    Detlef Weigel
    Article
    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Plant
    Mutation
    Promoter
    UBQ10
    Available since
    Jan. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104438-plasmid-map-sequence-id-201144

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  19. pT7-AsCas12a_crRNA-site4 (MSP3511)

    Plasmid
    #160139
    Purpose
    T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #4
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41
    Insert
    AsCas12a crRNA with spacer #4 (spacer=GGAATCCCTTCTGCAGCACCTGG)
    Use
    In vitro transcription of sgrna from t7 promoter
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    Feb. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    160139-plasmid-map-sequence-id-333510

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  20. pActin5c-CD-dCas9m4-UDI-attB

    Plasmid
    #104879
    Purpose
    To generate point mutations using CRISPR/Cas9 system; encoded cytidine deaminase fused to deactived Cas9
    Depositor
    Christopher Potter
    Insert
    CD-dCas9(m4)-UDI
    Use
    CRISPR
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    104879-plasmid-map-sequence-id-209447

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Showing: 2901 - 2920 of 34841 results