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NarCas9_AAV

Plasmid

#192145

Purpose

Expresses NarCas9?and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wei et al Elife. 2022 Aug 12;11. pii: 77825. doi:

Insert

NarCas9

Use

AAV

Expression

Mammalian

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pPD_Pinx-1(1k + 50 bp)::Venus

Plasmid

#117421

Purpose

For analysis of AIB cell differentiation by tracking Venus fluorescence under the expression of Pinx-1 promoter

Depositor

Shohei Mitani

Article

Hori et al PLoS Genet. 2018 Jun 25;14(6):e1007477.

Insert

Venus

Expression

Worm

Promoter

inx-1

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pRS416 Gal-RNQ1 L94A

Plasmid

#18691

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Rnq1 L94A

Expression

Yeast

Mutation

Leucine 94 mutated to Alanine (L94A)

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
p3E ubb polyA

Plasmid

#195977

Purpose

Gateway p3E vector

Depositor

Alexa Burger, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

ubb:polyA (ubb Zebrafish)

Use

Other

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pT3-myr-AKT-HA

Plasmid

#31789

Depositor

Xin Chen

Article

Calvisi et al Gastroenterology. 2011 Mar;140(3):1071-8

Insert

Akt1 (Akt1 Mouse)

Tags

HA and Myr

Expression

Mammalian

Available Since

Nov. 16, 2011

Availability

Academic Institutions and Nonprofits only

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HA-SOCS1

Plasmid

#174571

Purpose

Human HA-tagged SOCS1 expression (N-term. tag) (CMV prom.)

Depositor

Mathew Garnett

Article

Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

Insert

HA-SOCS1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pD2sE_SC14

Plasmid

#219717

Purpose

DV2 stable E dimer

Depositor

Brian Kuhlman

Article

Kudlacek et al Sci Adv. 2021 Oct 15;7(42):eabg4084. doi

Insert

DV2 sE SC14

Tags

8xHis and human serum albumin (HSA)

Expression

Mammalian

Mutation

A259W, T262R, F279W, T280P

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-Cre

Plasmid

#198712

Purpose

For lentiviral expression of Cre recombinase in mammalian cells.

Depositor

Tyler Jacks

Article

Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

Insert

Pgk-Cre

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-mitoX4-mVenus

Plasmid

#185808

Purpose

Mitochondrial targetted (COX8) mVenus

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

mito-mVenus

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pX330-SpCas9-NG

Plasmid

#117919

Purpose

Expresses 3xFLAG-NLS-SpCas9-NG-NLS in mammalian cells.

Depositor

Osamu Nureki

Article

Nishimasu et al Science. 2018 Aug 30. pii: science.aas91

Insert

SpCas9-NG (L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)

Expression

Mammalian

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA Myc DBC1

Plasmid

#35096

Depositor

Osamu Hiraike

Article

Hiraike et al Br J Cancer. 2010 Mar 16;102(6):1061-7.

Insert

deleted in breast cancer 1 (CCAR2 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2012

Availability

Academic Institutions and Nonprofits only

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hOT-GFP

Plasmid

#67848

Purpose

mammalian expression of human oxytocin receptor with GFP tag

Depositor

Christian Gruber

Article

Koehbach et al Proc Natl Acad Sci U S A. 2013 Dec 24;11

Insert

oxytocin receptor (OXTR Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only
pSI-Target-AID-NG

Plasmid

#119861

Purpose

Expresses Target-AID-NG (NLS-nSpCas9-NG-NLS-SH3-3xFLAG-PmCDA1-NLS-UGI) in mammalian cells.

Depositor

Osamu Nureki

Article

Nishimasu et al Science. 2018 Aug 30. pii: science.aas91

Insert

nSpCas9-NG (D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)

Expression

Mammalian

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pLEX307-SARS-CoV-2-3CL

Plasmid

#160278

Purpose

Allows for constituitive mammalian expression of the SARS-CoV-2 3CL protease via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221_HDLBP

Plasmid

#154448

Purpose

For recombination of HDLBP

Depositor

Markus Landthaler

Article

Zinnall et al Nat Commun. 2022 May 18;13(1):2727. doi:

Insert

HDLBP (HDLBP Human)

Expression

Mammalian

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-MED1trunc-IDR-YFP-NLS

Plasmid

#145275

Purpose

Mammalian Expression of Nuclear MED1 truncated IDR-YFP with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

MED1-IDR truncated (MED1 Human)

Expression

Mammalian

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-MCS-MED1long-IDR-YFP-NLS

Plasmid

#145276

Purpose

Mammalian Expression of Nuclear MED1 long IDR-YFP with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

MED1-IDR long (MED1 Human)

Expression

Mammalian

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-wtYAP

Plasmid

#84009

Purpose

expresses wtYAP in mammalian cells under the control of a doxycycline-inducible promoter

Depositor

Stefano Piccolo

Article

Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5

Insert

YAP1 (siRNA insensitive) (YAP1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

TRE-CMV

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-mScarlet-Giantin

Plasmid

#196872

Purpose

Expresion of Giantin-fused to mScarlet. Used as Golgi marker

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

mScarlet-Giantin (GOLGB1 Synthetic, Human)

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-HOXD13(+8A)-IDR-mCherry-CRY2-NLS

Plasmid

#145262

Purpose

Mammalian Expression of Nuclear Opto HOXD13(+8A) IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13(+8A)-IDR (HOXD13 Human)

Expression

Mammalian

Mutation

A-repeat expanded

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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