We narrowed to 34,882 results for: CaS;

1. pActin5c-CD-dCas9m4-UDI-attB

   Plasmid

   #104879

   Purpose

   To generate point mutations using CRISPR/Cas9 system; encoded cytidine deaminase fused to deactived Cas9

   Depositor

   Christopher Potter

   Insert

   CD-dCas9(m4)-UDI
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-FLEXfrt-SaCas9-U6-sgGria2

   Plasmid

   #124868

   Purpose

   Mutagenesis of Gria2

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gria2 (Gria2 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. p1194-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIA4_FLAG_NLS

   Plasmid

   #129532

   Purpose

   Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIA4

   Depositor

   Erik Sontheimer

   Article

   Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

   Inserts

   codon-optimized AcrIIA4
   sgRNA_Rosa26
   

   Use

   AAV

   Tags

   FLAG/NLS

   Expression

   Mutation

   Promoter

   U6 and cytomegalovirus-enhancer chicken β-actin p…

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT7-AsCas12a_crRNA-site4 (MSP3511)

   Plasmid

   #160139

   Purpose

   T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #4

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Insert

   AsCas12a crRNA with spacer #4 (spacer=GGAATCCCTTCTGCAGCACCTGG)
   

   Use

   In vitro transcription of sgrna from t7 promoter

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBiFC-HA-hCaspase 2 prodomain-VC155

   Plasmid

   #49261

   Purpose

   Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

   Depositor

   Douglas Green

   Article

   Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

   Insert

   caspase-2 prodomain (CASP2 Human)
   

   Use

   Tags

   HA and split Venus C

   Expression

   Mammalian

   Mutation

   delete aa 148-435

   Promoter

   CMV

   Available since

   Dec. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pQCasTns(Vch)-entry(BsaI)

   Plasmid

   #190273

   Purpose

   Expression of CRISPR-associated transposases

   Depositor

   Sheng Yang

   Article

   Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

   Insert

   VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(BsaI)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   no

   Promoter

   Available since

   Dec. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAC95-pmax-dCas9VP160-2A-neo

   Plasmid

   #48227

   Purpose

   dCas9VP160-2A-neo (neo/G418-selectable) on pmax expression vector. Note: This is being tested.

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-neo
   

   Use

   CRISPR

   Tags

   2A-neo, HA Tag, and VP160

   Expression

   Mammalian

   Mutation

   D10A;H840A (catalytically inactive)

   Promoter

   CAGGS

   Available since

   Sept. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-hIP-dCas9-BSD

   Plasmid

   #183231

   Purpose

   Lentiviral vectors with the human insulin promoter driving expression of dCas9, in addition to a U6-driven sgRNA

   Depositor

   Jorge Ferrer

   Article

   Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Human insulin promoter

   Available since

   Oct. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV-hCas9-T2A-mStrawberry

   Plasmid

   #187224

   Purpose

   Expresses human Cas9 nuclease and mStrawberry via T2A linker under control of a CMV promoter.

   Depositor

   Jennifer Mitchell

   Article

   Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Inserted T2A-mStrawberry at 3' terminal of M…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Anti-CASPR2 [K67/25R]

    Plasmid

    #177446

    Purpose

    Mammalian Expression Plasmid of anti-CASPR2 (Human). Derived from hybridoma K67/25.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-CASPR2 (Homo sapiens) recombinant mouse monoclonal antibody (CNTNAP2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Nov. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX551-miniCMV-SpCas9D10A nickase

    Plasmid

    #216735

    Purpose

    Expresses SpCas9-D10A nickase from a miniCMV promoter. For AAV packaging. Derived from pX551-miniCMV-SpCas9, which was a gift from Alex Hewitt (Addgene #107031)

    Depositor

    Vincent Dion

    Article

    Alvaro et al bioRxiv

    Insert

    Cas9-D10A nickase
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    T7 and mini-CMV

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. 1137=Hsp70Bb-Cas9[dsRed]

    Plasmid

    #149424

    Purpose

    The piggyBac plasmid harboring Hsp70Bb-Cas9-T2A-eGFP-p10 and Opie2-dsRed-SV10 (marker gene)

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    eGFP

    Expression

    Insect

    Mutation

    Promoter

    Dmel Hsp70Bb

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZ147-BvCas12b-sgRNA-scaffold

    Plasmid

    #122447

    Purpose

    Expresses sgRNA for BvCas12b in mammalian cells

    Depositor

    Feng Zhang

    Article

    Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

    Insert

    U6-BvCas12b-sgRNA-scaffold, CMV-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-dSaCas9-KRAB-blast

    Plasmid

    #191654

    Purpose

    Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain for gene silencing and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Article

    Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

    Insert

    dSaCas9-KRAB
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Expression scaffold for dCas9+sgRNA

    Plasmid

    #171799

    Purpose

    PICASSO: Expression scaffold for second subcloning step to properly co-express paired dCas9-fusion+sgRNA

    Depositor

    Stephen Elledge

    Article

    Barber et al Mol Cell. 2021 Aug 4. pii: S1097-2765(21

    Insert

    PICASSO: expression scaffold for proper dCas9-fusion + sgRNA expression
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVCERC-5 Pvan-dcas9 (Sth3)

    Plasmid

    #133319

    Purpose

    expresses dcas9 from Streptococcus thermophilus #3; induced with vanillate; for homologous recombination at the vanillate locus in Caulobacter crescentus; tetracycline resistance

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    dcas9 (Streptococcus thermophilus #3)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    vanillate

    Available since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTripz Casp1-IRES-mCherry

    Plasmid

    #187294

    Purpose

    This is Caspase1 from mouse (NM_009807.2) with its CARD domain replaced by the FKBP-V domain (dimerising domain).

    Depositor

    Alpha Yap

    Article

    Bonfim-Melo et al Cell Rep. 2022 Feb 1;38(5):110316. doi:

    Insert

    Caspase1 (Casp1 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBiFC-HA-hCaspase 2 CARD-VC155

    Plasmid

    #49259

    Purpose

    Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

    Depositor

    Douglas Green

    Article

    Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

    Insert

    Caspase-2 CARD (CASP2 Human)
    

    Use

    Tags

    HA and split Venus C

    Expression

    Mammalian

    Mutation

    deleted aa 123-435

    Promoter

    CMV

    Available since

    Dec. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pCF287_EFS-TiCas9-P2A-Puro

    Plasmid

    #211646

    Purpose

    EFS-TiCas9-P2A-Puro. Tamoxifen-inducible SpyCas9.

    Depositor

    Christof Fellmann

    Article

    Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

    Insert

    Tamoxifen-inducible SpyCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAH-CTX1-rhadCas9-native

    Plasmid

    #129392

    Purpose

    Derived from pAH-CTX1-rha. The native Streptococcus pyogenes dCas9 gene cloned downstream of PrhaBAD. Non-codon-optimized dCas9 version of pAH-CTX1-rhadCas9.

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    S. pyogenes dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Aspartate 10 to Alanine, Histidine 840 to Alanine

    Promoter

    PrhaBAD

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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