We narrowed to 3,169 results for: EED

1. pAG219 RIPK4 3'UTR mut

   Plasmid

   #12049

   Depositor

   David Bartel

   Article

   Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

   Insert

   N20 3'UTR mut (RIPK4 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   Mutations in microRNA recognition sites (seed mat…

   Available Since

   June 8, 2006

   Availability

   Academic Institutions and Nonprofits only

2. pAG87 DUXAP6 3'UTR mut

   Plasmid

   #12060

   Depositor

   David Bartel

   Article

   Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

   Insert

   N6 3'UTR mut (DUXAP6 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   Mutations in microRNA recognition sites (seed mat…

   Available Since

   June 2, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCLV3Cas-LT2

   Plasmid

   #210550

   Purpose

   Binary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT1 reporter

   Depositor

   Ortrun Mittelsten Scheid

   Article

   Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

   Insert

   CLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT2::mCherrySSM
   

   Expression

   Plant

   Mutation

   1bp insertion after AAVS1 target sequence that di…

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. psiCHECK2 RPS28 UTR1 SCR

   Plasmid

   #203158

   Purpose

   RPS28 UTR luciferase assay plasmid (SCR UTR1, sites 1 and 2)

   Depositor

   Susan Baserga

   Article

   Bryant et al Nucleic Acids Res. 2024 Feb 28;52(4):198

   Insert

   RPS28 UTR (RPS28 Human)
   

   Use

   Luciferase

   Mutation

   scrambled the nucleotides in the two MIR-28 bindi…

   Available Since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLP-pTac-himar-apraR

   Plasmid

   #199995

   Purpose

   Landing Pad with T7 RNAP circuit variant T15. pIP1443 seed promoter. pTac-himar transposase. In dap auxotrophic conjugation donor. This material contains 2 plasmids. For more information look below.

   Depositor

   Farren Isaacs

   Article

   Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

   Insert

   Landing Pads
   

   Expression

   Bacterial

   Mutation

   himar frameshift

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ265N2

   Plasmid

   #174001

   Purpose

   Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-UNG for C-G base editing

   Depositor

   Yiping Qi

   Article

   Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

   Insert

   rAPOBEC1-zCas9(D10A)-SpRY-UNG
   

   Use

   CRISPR

   Tags

   3X FLAG, NLS

   Expression

   Plant

   Mutation

   In zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQ265O2

   Plasmid

   #174002

   Purpose

   Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-rXRCC1 for C-G base editing

   Depositor

   Yiping Qi

   Article

   Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

   Insert

   rAPOBEC1-zCas9(D10A)-SpRY-rXRCC1
   

   Use

   CRISPR

   Tags

   3X FLAG, NLS

   Expression

   Plant

   Mutation

   In zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQ265L2

   Plasmid

   #174000

   Purpose

   Gateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY for C-G base editing

   Depositor

   Yiping Qi

   Article

   Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

   Insert

   UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY
   

   Use

   CRISPR

   Tags

   3X FLAG, NLS

   Expression

   Plant

   Mutation

   In rAPOBEC1: R33A; in zCas9(D10A)-SpRY: D10A, A61…

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYI006

   Plasmid

   #170840

   Purpose

   FRO6p::GUSPlus::tOCS in pFP100

   Depositor

   Christian Fankhauser

   Article

   Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

   Insert

   FRO6p::GUSPlus::tOCS
   

   Expression

   Plant

   Promoter

   FRO6

   Available Since

   Oct. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPR2_IHF_Opt

    Plasmid

    #149395

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with "optimized" IHFprox site

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Expression

    Bacterial

    Mutation

    IHFprox site is optimized at key positions needed…

    Promoter

    pBAD

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNB101

    Plasmid

    #169110

    Purpose

    Feedback-controlled AraC circuit

    Depositor

    Edwin Wintermute

    Article

    Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

    Insert

    araC (araC Escherichia coli K12 MG1655)
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Codon optimized for E. coli

    Available Since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMRBad-Z-C-DiB-RM

    Plasmid

    #168477

    Purpose

    C-fragment of the DiB-RM‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168475: pET11a-Z-N-DiB-RM to obtain DiB-RM‐split-Zip protein

    Depositor

    Jens Meiler

    Article

    Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100

    Insert

    Leucine Zipper + C-fragment of DiB-RM
    

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET11a-Z-N-DiB-RM

    Plasmid

    #168475

    Purpose

    N-fragment of the DiB-RM‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168477: pMRBad-Z-C-DiB-RM to obtain DiB-RM‐split-Zip protein

    Depositor

    Jens Meiler

    Article

    Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100

    Insert

    N-fragment of DiB-RM + Leucine Zipper
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRWJB006

    Plasmid

    #160113

    Purpose

    Destination vector expressing plant-codon-optimized Cas9 under 35S promoter (BamHI and NotI), with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free

    Depositor

    Detlef Weigel

    Article

    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial and Plant

    Promoter

    35S

    Available Since

    Feb. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRWJB004

    Plasmid

    #160112

    Purpose

    Destination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter (BamHI and NotI), with gRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;

    Depositor

    Detlef Weigel

    Article

    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial and Plant

    Promoter

    UBQ10

    Available Since

    Feb. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVH009

    Plasmid

    #80399

    Purpose

    Control plasmid with no anti-scaffold feedback. Contains pBAD-CusR (response regulator) and pCusR-YFP-AAV.

    Depositor

    Richard Murray

    Article

    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

    Inserts

    3xFLAG-Venus YFP-AAV
    CusR (cusR )
    AraC (araC )
    

    Use

    Synthetic Biology

    Tags

    C-terminal LZx domain and N-terminal 3xFLAG tag, …

    Expression

    Bacterial

    Available Since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPAt2S3 (GB0029)

    Plasmid

    #68162

    Purpose

    Provides the Arabidopsis thaliana Seed-specific promoter 2S3 as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    At2S3 promoter
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pC015 - dLwCas13a-NF

    Plasmid

    #91905

    Purpose

    Expresses negative-feedback dLwCas13a for mammalian RNA targeting/imaging

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    dLwCas13a
    

    Use

    CRISPR

    Tags

    NLS and eGFP, ZF CCR5TC, KRAB-A, NLS

    Expression

    Mammalian

    Mutation

    R474A and R1046A

    Promoter

    CAG

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-ihSyn1-tTA

    Plasmid

    #99120

    Purpose

    An AAV genome that expresses tTA from the hSyn1 promoter that contains a positive feedback loop for amplifed expression of the tTA

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Has Service

    AAV1

    Insert

    tTA
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    *(see below)

    Promoter

    ihSyn1

    Available Since

    Aug. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPPED

    Plasmid

    #162468

    Purpose

    Destination Expression vector for dicot plant prime editing

    Depositor

    Adam Bogdanove

    Article

    Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

    Inserts

    Cas9H840A
    attR gateway destination cassette
    

    Tags

    MLV reverse transcriptase

    Expression

    Plant

    Mutation

    Change histidine 840 to Alanine based sequence o…

    Promoter

    2x35S

    Available Since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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