We narrowed to 13,167 results for: Mpl;

1. SPLINTR GFP v1 backbone

   Plasmid

   #179777

   Purpose

   SPLINTR backbone vector

   Depositor

   Mark Dawson

   Article

   Fennell et al Nature. 2022 Jan;601(7891):125-131. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. HC078 (3xHA-Atg13)

   Plasmid

   #59544

   Purpose

   Expresses yeast Atg13 under the endogenous promoter with a 3xHA N-terminal tag

   Depositor

   Daniel Klionsky

   Article

   Cheong et al Mol Biol Cell. 2008 Feb;19(2):668-81. Ep

   Insert

   ATG13 (ATG13 Budding Yeast)
   

   Use

   Tags

   3xHA

   Expression

   Bacterial and Yeast

   Mutation

   AvrII site created after start codon

   Promoter

   ATG13

   Available since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

3. ATG9A-2STrep

   Plasmid

   #210359

   Purpose

   Expresses ATG9A fused with 2Strep in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   ATG9A (ATG9A Human)
   

   Use

   Tags

   2Strep

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiviral shRNA GEF-H1

   Plasmid

   #21477

   Depositor

   Richard Huganir

   Article

   Kang et al Proc Natl Acad Sci U S A. 2009 Mar 3. 10

   Insert

   rho/rac guanine nucleotide exchange factor (GEF) 2 (Arhgef2 Rat)
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

5. pAAV-SynI-CreOn-FlpOff-EGFP

   Plasmid

   #176274

   Purpose

   Viral vector for expression of EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

   Depositor

   Martin Riccomagno

   Article

   Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   EGFP
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human Synapsin I

   Available since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. CaV1.3e[8a,11,31b,Δ32,42a] mut

   Plasmid

   #26576

   Depositor

   Diane Lipscombe

   Article

   Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

   Insert

   CACNA1D (Cacna1d Rat)
   

   Use

   Tags

   6XHis and V5

   Expression

   Mammalian

   Mutation

   Contains exons 8a, 11, 31b and 42a.Deletion of ex…

   Promoter

   Available since

   Jan. 31, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pCas9-CAT

   Plasmid

   #80323

   Purpose

   Encodes Cas9 and chloramphenicol acetyltransferase (CAT)

   Depositor

   Sebastian Lourido

   Article

   Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

   Inserts

   Cas9
   Chloramphenicol acetyltransferase
   

   Use

   CRISPR

   Tags

   3X FLAG and NLS

   Expression

   Mutation

   Promoter

   TgTUB1

   Available since

   Sept. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. SpeCas9-HF

   Plasmid

   #192141

   Purpose

   Expresses highly specific SpeCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

   Insert

   SpeCas9-HF
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   SpeCas9-HF (R247A, N415A, S421A)

   Promoter

   Available since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMDJ112 - pEXP(HygroR, 5605)[Phsp-16.41 | eFlp recombinase (PATCs) | tbb-2 UTR]

   Plasmid

   #159811

   Purpose

   Optimized enzyme for genome editing in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP(HygroR, 5605)[Phsp-16.41 | eFlp recombinase (PATCs) | tbb-2 UTR]
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-Flex-mRuby3-2A

    Plasmid

    #203846

    Purpose

    Neuron specific expression of a Cre-dependent mRuby3

    Depositor

    Ivo Spiegel

    Article

    Cohen-Kashi Malina et al Neuron. 2021 Jul 7;109(13):2150-2164.e5.

    Insert

    mRuby3
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pClneoMyc human CIN85

    Plasmid

    #47935

    Purpose

    expresses Myc tagged human CIN85 in mammalian cells

    Depositor

    Yutaka Hata

    Article

    Kawata et al J Biochem. 2006 May;139(5):931-9.

    Insert

    CIN85 (SH3KBP1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    contains amino acids 2-665 of human CIN85; D114G

    Promoter

    CMV

    Available since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR_L1-miniTurbo-NES-YFP-STOP-L2

    Plasmid

    #127359

    Purpose

    gateway entry vector for expressing cytosolic miniTurbo-YFP under a promoter of choice

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NES, V5, and YFP

    Expression

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    no promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-N1-PHD3

    Plasmid

    #21402

    Depositor

    Eric Metzen

    Article

    Metzen et al J Cell Sci. 2003 Apr 1. 116(Pt 7):1319-2

    Insert

    EGLN3 (EGLN3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pTS102 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-SUMOstar-MCS

    Plasmid

    #199355

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-SUMOstar-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TagBFP-P2A-ALFA-SUMOstar

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. NSP3C-Flag

    Plasmid

    #210349

    Purpose

    Expresses the C terminal of SARS-CoV-2 NSP3 fused with Flag in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    C terminal of SARS-CoV-2 NSP3 (ORF1ab )
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-dSaCas9-KRAB-blast

    Plasmid

    #191654

    Purpose

    Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain for gene silencing and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Article

    Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

    Insert

    dSaCas9-KRAB
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKLV2-U6gRNA5(GFP)-PGKpuro2ABFP-W

    Plasmid

    #200475

    Purpose

    Lentiviral vector expressing gRNA targeting GFP

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FLAG-Med29 pBacPAK8

    Plasmid

    #15369

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

    Insert

    Med29 (MED29 Human)
    

    Use

    Baculovirus construction

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pBMN mCherry-TAX1BP1(V142S/ΔUBD)

    Plasmid

    #119690

    Purpose

    For mammalian expression of LIR mutant TAX1BP1 without its UBD

    Depositor

    Michael Lazarou

    Article

    Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

    Insert

    TAX1BP1 (TAX1BP1 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    V142S & UBD deletion (1-755), See depositor c…

    Promoter

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PAmCherry1-C1-LACT-C2

    Plasmid

    #202682

    Purpose

    Photoactivatable lipid biosensor

    Depositor

    Gerry Hammond

    Article

    Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

    Insert

    PAmCherry1: Bos taurus MFGE8 (274-431) (MFGE8 Bovine)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTS112 pHAGE2 CMVtetO2 MCS-TEV-22xGS-Alfa-P2A-TagBFP

    Plasmid

    #199365

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-ALFA-P2A-BFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TEV-ALFA-P2A-TagBFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA 3.1-nsp7-FLAG

    Plasmid

    #201027

    Purpose

    Expresses C-terminally FLAG tagged SARS-CoV-2 nsp7 in mammalian.

    Depositor

    Sharon Rozovsky

    Article

    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

    Insert

    non-structural protein 7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    

    Use

    Tags

    8xHis and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-hSyn-mCherry.3xFLAG.NOS1AP-WPRE

    Plasmid

    #127864

    Purpose

    pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP fusion protein under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

    Insert

    Nos1ap (Nos1ap Mouse)
    

    Use

    AAV

    Tags

    3xFLAG and mCherry

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. p255 pPr-CREM/Ins/CMV-STOP-luc

    Plasmid

    #8408

    Purpose

    Modified cre expressed from probasin, separated from the Cre-dependent CMV-STOP-luc cassette by insulators.

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

    Insert

    CREM/insulators/lox/luciferase
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    modified Cre

    Promoter

    Probasin, CMV

    Available since

    April 26, 2006

    Availability

    Academic Institutions and Nonprofits only

25. pGEX-4T-1-β4 hinge-ear

    Plasmid

    #196692

    Purpose

    Expresses GST-AP-4 β4 hinge-ear (residues 534-739) in bacteria

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

    Insert

    AP-4 β4 hinge-ear (534-739) (AP4B1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. shPBRM1#32-Tet-pLKO-puro

    Plasmid

    #107399

    Purpose

    Lentiviral expression of shRNA targeting PBRM

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    shRNA targeting PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. HOXB8 (murine) HIS-tag pET

    Plasmid

    #8540

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXB8 (Hoxb8 Mouse)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    starts at amino acid #2 (Ser)

    Promoter

    Available since

    Aug. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

28. pSKA413

    Plasmid

    #80381

    Purpose

    Constitutive CcaS and CcaR with gfpmut3 under the cpcG2Δ59 promoter.

    Depositor

    Mustafa Khammash

    Article

    Milias-Argeitis et al Nat Commun. 2016 Aug 26;7:12546. doi: 10

    Inserts

    ccaS (complement)
    ccaR (complement)
    gfpmut3
    

    Use

    Synthetic Biology; Optogenetics

    Tags

    FLAG tag

    Expression

    Bacterial

    Mutation

    Promoter

    ccaR and cpcG2Δ59

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. shPBRM1#131-Tet-pLKO-puro

    Plasmid

    #107400

    Purpose

    Lentiviral expression of shRNA targeting PBRM

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    shRNA targeting PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEGFP-N1-PHD1

    Plasmid

    #21400

    Depositor

    Eric Metzen

    Article

    Metzen et al J Cell Sci. 2003 Apr 1. 116(Pt 7):1319-2

    Insert

    EGLN2 (EGLN2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

31. pENTR-D-TOPO-kcnn3

    Plasmid

    #164959

    Purpose

    Zebrafish kcnn3 gene CDS

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

    Insert

    kcnn3 (kcnn3 Zebrafish)
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSIN-U6-tracer_C9_1_-EF1a-Thy1.1-P2A-Neo

    Plasmid

    #191397

    Purpose

    sgRNA targeting alpha-satellites on human chromosome 9

    Depositor

    Albert Jeltsch

    Article

    Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

    Insert

    sgRNA targeting alpha satellites on human chromosome 9
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. 1.5 BV

    Plasmid

    #16048

    Depositor

    Robert Benezra

    Article

    Tournay et al Mol Cell Biol. 1996 May . 16(5):2418-30.

    Insert

    Id1 promoter region (Id1 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    -1585 to +88 of promoter

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

34. pCIneo-V5-SBP-HseIF4AI_AC

    Plasmid

    #148438

    Purpose

    Mammalian Expression of HseIF4AI

    Depositor

    Elisa Izaurralde

    Article

    Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

    Insert

    HseIF4AI (EIF4A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    3 silent mutations relative to NM_001416.2

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pETM6-RgTALsyn-HpaBC

    Plasmid

    #100949

    Purpose

    Expression of RgTALsyn, HpaB, and HpaC from inducible T7 promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

    Inserts

    RgTALsyn
    HpaB
    HpaC
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Silent mutation to remove internal NdeI site on T…

    Promoter

    T7

    Available since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCFJ2452 - [1-2] ENTR - Fluor - ce-mNeon(PATCs(900bp), NLS, no_atg, no stop)

    Plasmid

    #159854

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-mNeon(PATCs(900bp), NLS, no_atg, no stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEF6 VPS51-V5-6His

    Plasmid

    #175502

    Purpose

    V5 tagged VPS51 is expressed in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Perez-Victoria et al Mol Biol Cell. 2010 Oct 1;21(19):3386-95

    Insert

    VPS51 (VPS51 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    A661V- please see depositor comments

    Promoter

    Available since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEBTet-SNAP-CCCAP

    Plasmid

    #136798

    Purpose

    Mammalian expression of the centrosomal protein CCCAP N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-CCCAP (SDCCAG8 Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. px458_2A_GFP_sgRNA_G3BP2_G1

    Plasmid

    #127112

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    gRNA G3BP2 (G3BP2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pEGFP-C1-BBS4

    Plasmid

    #218713

    Purpose

    Expresses N-terminally EGFP-tagged BBS4 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

    Insert

    BBS4 (BBS4 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    I354T (t1110c)

    Promoter

    CMV

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge